Effect of NtDCN1 gene activated during embyogenesis on organogenesis in tobacco tissue culture
The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids f...
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| Veröffentlicht in: | Russian journal of plant physiology 2010, Vol.57 (1), p.118-123 |
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| container_title | Russian journal of plant physiology |
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| creator | Dolgikh, Yu. I Stepanova, A. Yu Osipova, E. S Tashpulatov, A. Sh Hosp, J Ribarits, A Heberle-Bors, E Touraev, A. M |
| description | The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids from microspores. In leaf disks and calli derived from tobacco lines with active and inactivated NtDCN1 gene, we studied induction of shoots and roots. A comparative analysis of tobacco line morphogenetic responses in vitro showed that NtDCN1 gene inactivation enhanced shoot formation and suppressed rhizogenesis, whereas this gene reactivation returned organogenesis processes to control level. Difference between lines was manifested only at a definite ratio between exogenous hormones supplied. The involvement of NtDCN1 gene in line responses to exogenous auxin is discussed. The results obtained permit a supposition that the NtDCN1 gene is critical for regulation not only somatic embryogenesis but also organogenesis. |
| doi_str_mv | 10.1134/S1021443710010164 |
| format | Article |
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I ; Stepanova, A. Yu ; Osipova, E. S ; Tashpulatov, A. Sh ; Hosp, J ; Ribarits, A ; Heberle-Bors, E ; Touraev, A. M</creator><creatorcontrib>Dolgikh, Yu. I ; Stepanova, A. Yu ; Osipova, E. S ; Tashpulatov, A. Sh ; Hosp, J ; Ribarits, A ; Heberle-Bors, E ; Touraev, A. M</creatorcontrib><description>The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids from microspores. In leaf disks and calli derived from tobacco lines with active and inactivated NtDCN1 gene, we studied induction of shoots and roots. A comparative analysis of tobacco line morphogenetic responses in vitro showed that NtDCN1 gene inactivation enhanced shoot formation and suppressed rhizogenesis, whereas this gene reactivation returned organogenesis processes to control level. Difference between lines was manifested only at a definite ratio between exogenous hormones supplied. The involvement of NtDCN1 gene in line responses to exogenous auxin is discussed. The results obtained permit a supposition that the NtDCN1 gene is critical for regulation not only somatic embryogenesis but also organogenesis.</description><identifier>ISSN: 1021-4437</identifier><identifier>EISSN: 1608-3407</identifier><identifier>DOI: 10.1134/S1021443710010164</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : SP MAIK Nauka/Interperiodica</publisher><subject>auxin ; Auxins ; Biomedical and Life Sciences ; Hormones ; Leaves ; Life Sciences ; Nicotiana tabacum ; NtDCN1 gene ; Organogenesis ; Plant Physiology ; Plant Sciences ; Research Papers ; Roots ; Shoots ; Somatic embryogenesis ; Tissue culture</subject><ispartof>Russian journal of plant physiology, 2010, Vol.57 (1), p.118-123</ispartof><rights>Pleiades Publishing, Ltd. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c297t-5f6042a12280e771d13f9a2fc1c6420b11bea1528b47dd548d2292634d9dbb773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1021443710010164$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1021443710010164$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,4022,27922,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Dolgikh, Yu. I</creatorcontrib><creatorcontrib>Stepanova, A. Yu</creatorcontrib><creatorcontrib>Osipova, E. S</creatorcontrib><creatorcontrib>Tashpulatov, A. Sh</creatorcontrib><creatorcontrib>Hosp, J</creatorcontrib><creatorcontrib>Ribarits, A</creatorcontrib><creatorcontrib>Heberle-Bors, E</creatorcontrib><creatorcontrib>Touraev, A. M</creatorcontrib><title>Effect of NtDCN1 gene activated during embyogenesis on organogenesis in tobacco tissue culture</title><title>Russian journal of plant physiology</title><addtitle>Russ J Plant Physiol</addtitle><description>The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids from microspores. In leaf disks and calli derived from tobacco lines with active and inactivated NtDCN1 gene, we studied induction of shoots and roots. A comparative analysis of tobacco line morphogenetic responses in vitro showed that NtDCN1 gene inactivation enhanced shoot formation and suppressed rhizogenesis, whereas this gene reactivation returned organogenesis processes to control level. Difference between lines was manifested only at a definite ratio between exogenous hormones supplied. The involvement of NtDCN1 gene in line responses to exogenous auxin is discussed. The results obtained permit a supposition that the NtDCN1 gene is critical for regulation not only somatic embryogenesis but also organogenesis.</description><subject>auxin</subject><subject>Auxins</subject><subject>Biomedical and Life Sciences</subject><subject>Hormones</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Nicotiana tabacum</subject><subject>NtDCN1 gene</subject><subject>Organogenesis</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Research Papers</subject><subject>Roots</subject><subject>Shoots</subject><subject>Somatic embryogenesis</subject><subject>Tissue culture</subject><issn>1021-4437</issn><issn>1608-3407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRSMEEqXwAazwkk3AYztxskSlPKSqLEq3RI4fUao0LraD1L_HURAbJFYzmnvvaOYkyTXgOwDK7jeACTBGOWAMGHJ2kswgx0VKGeansY9yOurnyYX3u9GE82yWfCyN0TIga9A6PC7WgBrdayRkaL9E0AqpwbV9g_S-PtpR8q1HtkfWNaL_HbQ9CrYWUloUWu8HjeTQhcHpy-TMiM7rq586T7ZPy_fFS7p6e35dPKxSSUoe0szkmBEBhBRYcw4KqCkFMRJkzgiuAWotICNFzbhSGSsUISXJKVOlqmvO6Ty5nfYenP0ctA_VvvVSd53otR18FZHwMisyXEYrTFbprPdOm-rg2r1wx2iqRpbVH5YxQ6aMP4w0tKt2dnB9_Ojf0M0UMsJWonGtr7YbgoHGWwiPx9NvzXx-OQ</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Dolgikh, Yu. I</creator><creator>Stepanova, A. Yu</creator><creator>Osipova, E. S</creator><creator>Tashpulatov, A. Sh</creator><creator>Hosp, J</creator><creator>Ribarits, A</creator><creator>Heberle-Bors, E</creator><creator>Touraev, A. M</creator><general>Dordrecht : SP MAIK Nauka/Interperiodica</general><general>SP MAIK Nauka/Interperiodica</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2010</creationdate><title>Effect of NtDCN1 gene activated during embyogenesis on organogenesis in tobacco tissue culture</title><author>Dolgikh, Yu. I ; Stepanova, A. Yu ; Osipova, E. S ; Tashpulatov, A. Sh ; Hosp, J ; Ribarits, A ; Heberle-Bors, E ; Touraev, A. 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Sh</creatorcontrib><creatorcontrib>Hosp, J</creatorcontrib><creatorcontrib>Ribarits, A</creatorcontrib><creatorcontrib>Heberle-Bors, E</creatorcontrib><creatorcontrib>Touraev, A. M</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Russian journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dolgikh, Yu. I</au><au>Stepanova, A. Yu</au><au>Osipova, E. S</au><au>Tashpulatov, A. Sh</au><au>Hosp, J</au><au>Ribarits, A</au><au>Heberle-Bors, E</au><au>Touraev, A. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of NtDCN1 gene activated during embyogenesis on organogenesis in tobacco tissue culture</atitle><jtitle>Russian journal of plant physiology</jtitle><stitle>Russ J Plant Physiol</stitle><date>2010</date><risdate>2010</risdate><volume>57</volume><issue>1</issue><spage>118</spage><epage>123</epage><pages>118-123</pages><issn>1021-4437</issn><eissn>1608-3407</eissn><abstract>The role of NtDCN1 gene in organogenesis in the culture of tobacco (Nicotiana tabacum L.) somatic tissues was studied. This gene is specifically expressed in tobacco microspores induced for somatic embryogenesis. This gene knockout resulted in a disturbance of formation and development of embyoids from microspores. In leaf disks and calli derived from tobacco lines with active and inactivated NtDCN1 gene, we studied induction of shoots and roots. A comparative analysis of tobacco line morphogenetic responses in vitro showed that NtDCN1 gene inactivation enhanced shoot formation and suppressed rhizogenesis, whereas this gene reactivation returned organogenesis processes to control level. Difference between lines was manifested only at a definite ratio between exogenous hormones supplied. The involvement of NtDCN1 gene in line responses to exogenous auxin is discussed. The results obtained permit a supposition that the NtDCN1 gene is critical for regulation not only somatic embryogenesis but also organogenesis.</abstract><cop>Dordrecht</cop><pub>Dordrecht : SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1021443710010164</doi><tpages>6</tpages></addata></record> |
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| subjects | auxin Auxins Biomedical and Life Sciences Hormones Leaves Life Sciences Nicotiana tabacum NtDCN1 gene Organogenesis Plant Physiology Plant Sciences Research Papers Roots Shoots Somatic embryogenesis Tissue culture |
| title | Effect of NtDCN1 gene activated during embyogenesis on organogenesis in tobacco tissue culture |
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