Establishment of a monoclonal anti-pan HLA class I antibody suitable for immunostaining of formalin-fixed tissue: Unusually high frequency of down-regulation in breast cancer tissues
A novel monoclonal anti‐pan human leukocyte antigen (HLA) class I heavy chain antibody, EMR8‐5, was established. It could detect HLA‐A, ‐B, and ‐C antigens in formalin‐fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8‐5 antibody, various cancer tissues from 246 cases we...
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| Veröffentlicht in: | Pathology international 2012-05, Vol.62 (5), p.303-308 |
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| creator | Torigoe, Toshihiko Asanuma, Hiroko Nakazawa, Emiri Tamura, Yasuaki Hirohashi, Yoshihiko Yamamoto, Eri Kanaseki, Takayuki Hasegawa, Tadashi Sato, Noriyuki |
| description | A novel monoclonal anti‐pan human leukocyte antigen (HLA) class I heavy chain antibody, EMR8‐5, was established. It could detect HLA‐A, ‐B, and ‐C antigens in formalin‐fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8‐5 antibody, various cancer tissues from 246 cases were examined for HLA class I expression. It was found that HLA class I expression was decreased in 20% to 42% of the cases of lung cancer, hepatocellular carcinoma, colon cancer, renal cell carcinoma, and urothelial carcinoma. In contrast, 85% of breast cancer cases had loss of or decreased HLA class I expression. Of the 35 breast cancer cases that had decreased HLA class I heavy chain expression, 33 (94%) also had decreased beta2‐microglobulin expression detected by immunohistochemical staining. It was suggested that HLA class I down‐regulation might be a common characteristic of breast cancer mostly caused by the down‐regulation of beta2‐microglobulin expression. |
| doi_str_mv | 10.1111/j.1440-1827.2012.02789.x |
| format | Article |
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It could detect HLA‐A, ‐B, and ‐C antigens in formalin‐fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8‐5 antibody, various cancer tissues from 246 cases were examined for HLA class I expression. It was found that HLA class I expression was decreased in 20% to 42% of the cases of lung cancer, hepatocellular carcinoma, colon cancer, renal cell carcinoma, and urothelial carcinoma. In contrast, 85% of breast cancer cases had loss of or decreased HLA class I expression. Of the 35 breast cancer cases that had decreased HLA class I heavy chain expression, 33 (94%) also had decreased beta2‐microglobulin expression detected by immunohistochemical staining. It was suggested that HLA class I down‐regulation might be a common characteristic of breast cancer mostly caused by the down‐regulation of beta2‐microglobulin expression.</description><identifier>ISSN: 1320-5463</identifier><identifier>EISSN: 1440-1827</identifier><identifier>DOI: 10.1111/j.1440-1827.2012.02789.x</identifier><identifier>PMID: 22524657</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Antibodies ; Antibodies, Monoclonal - immunology ; antigen presentation ; Antigens, Neoplasm - immunology ; beta 2-microglobulin ; beta 2-Microglobulin - metabolism ; beta2-microglobulin ; Biomarkers, Tumor ; Breast cancer ; Breast Neoplasms - immunology ; Breast Neoplasms - pathology ; Colon cancer ; Down-Regulation ; Female ; Formaldehyde ; Genes, MHC Class I - immunology ; Hepatocellular carcinoma ; Histocompatibility antigen HLA ; Histocompatibility Antigens Class I - immunology ; Humans ; Immune escape ; Immunohistochemistry - methods ; Lung cancer ; MHC class I ; Paraffin ; renal cell carcinoma ; Tissue Fixation ; urothelial carcinoma</subject><ispartof>Pathology international, 2012-05, Vol.62 (5), p.303-308</ispartof><rights>2012 The Authors. Pathology International © 2012 Japanese Society of Pathology and Blackwell Publishing Asia Pty Ltd</rights><rights>2012 The Authors. Pathology International © 2012 Japanese Society of Pathology and Blackwell Publishing Asia Pty Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5599-df184c5355395bfe198b5bbeb1d180e910bf5fcea668d2b14266dfb7988492873</citedby><cites>FETCH-LOGICAL-c5599-df184c5355395bfe198b5bbeb1d180e910bf5fcea668d2b14266dfb7988492873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1827.2012.02789.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1827.2012.02789.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22524657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torigoe, Toshihiko</creatorcontrib><creatorcontrib>Asanuma, Hiroko</creatorcontrib><creatorcontrib>Nakazawa, Emiri</creatorcontrib><creatorcontrib>Tamura, Yasuaki</creatorcontrib><creatorcontrib>Hirohashi, Yoshihiko</creatorcontrib><creatorcontrib>Yamamoto, Eri</creatorcontrib><creatorcontrib>Kanaseki, Takayuki</creatorcontrib><creatorcontrib>Hasegawa, Tadashi</creatorcontrib><creatorcontrib>Sato, Noriyuki</creatorcontrib><title>Establishment of a monoclonal anti-pan HLA class I antibody suitable for immunostaining of formalin-fixed tissue: Unusually high frequency of down-regulation in breast cancer tissues</title><title>Pathology international</title><addtitle>Pathol Int</addtitle><description>A novel monoclonal anti‐pan human leukocyte antigen (HLA) class I heavy chain antibody, EMR8‐5, was established. It could detect HLA‐A, ‐B, and ‐C antigens in formalin‐fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8‐5 antibody, various cancer tissues from 246 cases were examined for HLA class I expression. It was found that HLA class I expression was decreased in 20% to 42% of the cases of lung cancer, hepatocellular carcinoma, colon cancer, renal cell carcinoma, and urothelial carcinoma. In contrast, 85% of breast cancer cases had loss of or decreased HLA class I expression. Of the 35 breast cancer cases that had decreased HLA class I heavy chain expression, 33 (94%) also had decreased beta2‐microglobulin expression detected by immunohistochemical staining. It was suggested that HLA class I down‐regulation might be a common characteristic of breast cancer mostly caused by the down‐regulation of beta2‐microglobulin expression.</description><subject>Antibodies</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>antigen presentation</subject><subject>Antigens, Neoplasm - immunology</subject><subject>beta 2-microglobulin</subject><subject>beta 2-Microglobulin - metabolism</subject><subject>beta2-microglobulin</subject><subject>Biomarkers, Tumor</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - immunology</subject><subject>Breast Neoplasms - pathology</subject><subject>Colon cancer</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Formaldehyde</subject><subject>Genes, MHC Class I - immunology</subject><subject>Hepatocellular carcinoma</subject><subject>Histocompatibility antigen HLA</subject><subject>Histocompatibility Antigens Class I - immunology</subject><subject>Humans</subject><subject>Immune escape</subject><subject>Immunohistochemistry - methods</subject><subject>Lung cancer</subject><subject>MHC class I</subject><subject>Paraffin</subject><subject>renal cell carcinoma</subject><subject>Tissue Fixation</subject><subject>urothelial carcinoma</subject><issn>1320-5463</issn><issn>1440-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEUhUcIREvhFZCXbCb4Z-zxILGoqpJEigKLFpaWPWMnDh472DNq8mI8H54mZF1vfHXv-Y6te4oCIDhD-XzezVBVwRJxXM8wRHgGcc2b2eFVcX0ZvM41wbCkFSNXxbuUdhCimjD4trjCmOKK0fq6-HufBqmcTdte-wEEAyTogw-tC146IP1gy730YLG6Ba2TKYHlc1OF7gjSaCdYAxMisH0_-pDdrLd-Mznlbi-d9aWxB92BwaY06i_g0Y9plM4dwdZutsBE_WfUvj1OSBeefBn1ZnRysMED64GKWqYBtNK3Op5N0vvijZEu6Q_n-6Z4_Hb_cLcoV9_ny7vbVdlS2jRlZxCvWkooJQ1VRqOGK6qUVqhDHOoGQWWoabVkjHdYoQoz1hlVN5xXDeY1uSk-nXz3MeRfpkH0NrXaOel1GJNAeaUNZQ0jL5DChmIGCc9SfpK2MaQUtRH7aHsZj1kkpoDFTkw5iilHMQUsngMWh4x-PL8yql53F_B_olnw9SR4sk4fX2wsfizXU5X58sTbNOjDhZfxt2A1qan4tZ6LNVpA8jBH4if5B1t5xwE</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Torigoe, Toshihiko</creator><creator>Asanuma, Hiroko</creator><creator>Nakazawa, Emiri</creator><creator>Tamura, Yasuaki</creator><creator>Hirohashi, Yoshihiko</creator><creator>Yamamoto, Eri</creator><creator>Kanaseki, Takayuki</creator><creator>Hasegawa, Tadashi</creator><creator>Sato, Noriyuki</creator><general>Blackwell Publishing Asia</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201205</creationdate><title>Establishment of a monoclonal anti-pan HLA class I antibody suitable for immunostaining of formalin-fixed tissue: Unusually high frequency of down-regulation in breast cancer tissues</title><author>Torigoe, Toshihiko ; Asanuma, Hiroko ; Nakazawa, Emiri ; Tamura, Yasuaki ; Hirohashi, Yoshihiko ; Yamamoto, Eri ; Kanaseki, Takayuki ; Hasegawa, Tadashi ; Sato, Noriyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5599-df184c5355395bfe198b5bbeb1d180e910bf5fcea668d2b14266dfb7988492873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antibodies</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>antigen presentation</topic><topic>Antigens, Neoplasm - immunology</topic><topic>beta 2-microglobulin</topic><topic>beta 2-Microglobulin - metabolism</topic><topic>beta2-microglobulin</topic><topic>Biomarkers, Tumor</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - immunology</topic><topic>Breast Neoplasms - pathology</topic><topic>Colon cancer</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Formaldehyde</topic><topic>Genes, MHC Class I - immunology</topic><topic>Hepatocellular carcinoma</topic><topic>Histocompatibility antigen HLA</topic><topic>Histocompatibility Antigens Class I - immunology</topic><topic>Humans</topic><topic>Immune escape</topic><topic>Immunohistochemistry - methods</topic><topic>Lung cancer</topic><topic>MHC class I</topic><topic>Paraffin</topic><topic>renal cell carcinoma</topic><topic>Tissue Fixation</topic><topic>urothelial carcinoma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torigoe, Toshihiko</creatorcontrib><creatorcontrib>Asanuma, Hiroko</creatorcontrib><creatorcontrib>Nakazawa, Emiri</creatorcontrib><creatorcontrib>Tamura, Yasuaki</creatorcontrib><creatorcontrib>Hirohashi, Yoshihiko</creatorcontrib><creatorcontrib>Yamamoto, Eri</creatorcontrib><creatorcontrib>Kanaseki, Takayuki</creatorcontrib><creatorcontrib>Hasegawa, Tadashi</creatorcontrib><creatorcontrib>Sato, Noriyuki</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Pathology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torigoe, Toshihiko</au><au>Asanuma, Hiroko</au><au>Nakazawa, Emiri</au><au>Tamura, Yasuaki</au><au>Hirohashi, Yoshihiko</au><au>Yamamoto, Eri</au><au>Kanaseki, Takayuki</au><au>Hasegawa, Tadashi</au><au>Sato, Noriyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a monoclonal anti-pan HLA class I antibody suitable for immunostaining of formalin-fixed tissue: Unusually high frequency of down-regulation in breast cancer tissues</atitle><jtitle>Pathology international</jtitle><addtitle>Pathol Int</addtitle><date>2012-05</date><risdate>2012</risdate><volume>62</volume><issue>5</issue><spage>303</spage><epage>308</epage><pages>303-308</pages><issn>1320-5463</issn><eissn>1440-1827</eissn><abstract>A novel monoclonal anti‐pan human leukocyte antigen (HLA) class I heavy chain antibody, EMR8‐5, was established. It could detect HLA‐A, ‐B, and ‐C antigens in formalin‐fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8‐5 antibody, various cancer tissues from 246 cases were examined for HLA class I expression. It was found that HLA class I expression was decreased in 20% to 42% of the cases of lung cancer, hepatocellular carcinoma, colon cancer, renal cell carcinoma, and urothelial carcinoma. In contrast, 85% of breast cancer cases had loss of or decreased HLA class I expression. Of the 35 breast cancer cases that had decreased HLA class I heavy chain expression, 33 (94%) also had decreased beta2‐microglobulin expression detected by immunohistochemical staining. It was suggested that HLA class I down‐regulation might be a common characteristic of breast cancer mostly caused by the down‐regulation of beta2‐microglobulin expression.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>22524657</pmid><doi>10.1111/j.1440-1827.2012.02789.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Pathology international, 2012-05, Vol.62 (5), p.303-308 |
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| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Antibodies Antibodies, Monoclonal - immunology antigen presentation Antigens, Neoplasm - immunology beta 2-microglobulin beta 2-Microglobulin - metabolism beta2-microglobulin Biomarkers, Tumor Breast cancer Breast Neoplasms - immunology Breast Neoplasms - pathology Colon cancer Down-Regulation Female Formaldehyde Genes, MHC Class I - immunology Hepatocellular carcinoma Histocompatibility antigen HLA Histocompatibility Antigens Class I - immunology Humans Immune escape Immunohistochemistry - methods Lung cancer MHC class I Paraffin renal cell carcinoma Tissue Fixation urothelial carcinoma |
| title | Establishment of a monoclonal anti-pan HLA class I antibody suitable for immunostaining of formalin-fixed tissue: Unusually high frequency of down-regulation in breast cancer tissues |
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