Dynamic layer-by-layer self-assembly of multi-walled carbon nanotubes on quartz wool for on-line separation of lysozyme in egg white
The multi-walled carbon nanotubes (MWNTs) coated quartz wool (MWNTs/QW) prepared by dynamic layer-by-layer self-assembly was used as solid-phase extraction (SPE) absorbent for on-line separation and preconcentration of lysozyme in egg white. The coating procedures were performed continuously in a fl...
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| Veröffentlicht in: | Talanta (Oxford) 2012-05, Vol.94, p.104-110 |
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| creator | Du, Zhuo Zhang, Suling Zhou, Chanyuan Liu, Miao Li, Gongke |
| description | The multi-walled carbon nanotubes (MWNTs) coated quartz wool (MWNTs/QW) prepared by dynamic layer-by-layer self-assembly was used as solid-phase extraction (SPE) absorbent for on-line separation and preconcentration of lysozyme in egg white. The coating procedures were performed continuously in a flow system operated by a set of sequential injection devices. The quartz wool was placed in a microcolumn forming a loose packing to guarantee the minimized flow impedance and the intimate contact between proteins and absorbent surface. Various parameters affecting SPE efficiency including the volume, pH, ionic strength and flow rate of sample and eluent were systematically studied. The feasibility of the proposed method was validated by successfully applied to the separation of lysozyme in egg white. |
| doi_str_mv | 10.1016/j.talanta.2012.03.002 |
| format | Article |
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The coating procedures were performed continuously in a flow system operated by a set of sequential injection devices. The quartz wool was placed in a microcolumn forming a loose packing to guarantee the minimized flow impedance and the intimate contact between proteins and absorbent surface. Various parameters affecting SPE efficiency including the volume, pH, ionic strength and flow rate of sample and eluent were systematically studied. The feasibility of the proposed method was validated by successfully applied to the separation of lysozyme in egg white.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2012.03.002</identifier><identifier>PMID: 22608421</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adsorption ; Analytical chemistry ; Animals ; Biological and medical sciences ; Chemistry ; Chickens ; Dynamic layer-by-layer self-assembly ; Egg White - chemistry ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Interactions. Associations ; Intermolecular phenomena ; Lysozyme ; Molecular biophysics ; Multi-walled carbon nanotubes ; Muramidase - isolation & purification ; Nanotubes, Carbon - chemistry ; Osmolar Concentration ; Quartz - chemistry ; Quartz wool ; Solid Phase Extraction - methods ; Solid-phase extraction ; Wool - chemistry</subject><ispartof>Talanta (Oxford), 2012-05, Vol.94, p.104-110</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. 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The coating procedures were performed continuously in a flow system operated by a set of sequential injection devices. The quartz wool was placed in a microcolumn forming a loose packing to guarantee the minimized flow impedance and the intimate contact between proteins and absorbent surface. Various parameters affecting SPE efficiency including the volume, pH, ionic strength and flow rate of sample and eluent were systematically studied. The feasibility of the proposed method was validated by successfully applied to the separation of lysozyme in egg white.</description><subject>Adsorption</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chickens</subject><subject>Dynamic layer-by-layer self-assembly</subject><subject>Egg White - chemistry</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Interactions. Associations</subject><subject>Intermolecular phenomena</subject><subject>Lysozyme</subject><subject>Molecular biophysics</subject><subject>Multi-walled carbon nanotubes</subject><subject>Muramidase - isolation & purification</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Osmolar Concentration</subject><subject>Quartz - chemistry</subject><subject>Quartz wool</subject><subject>Solid Phase Extraction - methods</subject><subject>Solid-phase extraction</subject><subject>Wool - chemistry</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuOFCEYhYnROG3rI2jYmLih5FJUda2MGa_JJG50TYD6GelQRQ9QdmrWPri03erSFZd8nJ98B6HnjDaMsu71vik66LnohlPGGyoaSvkDtGG7XhAhe_EQbSgVAxlYS6_Qk5z3tBKCisfoivOO7lrONujnu3XWk7c46BUSMSv5vcEZgiM6Z5hMWHF0eFpC8eSoQ4ARW51MnPGs51gWAxnXw92iU7nHxxgDdjHVKxL8DDXpoJMuviI1Jqw53q8TYD9juL3Fx---wFP0yOmQ4dll3aJvH95_vf5Ebr58_Hz99oZYMchCOIyUiXbHO-56kAOzphVd34EZOympYb0chRx1LywdgIK10jFOuRk6N7TOiC16dc49pHi3QC5q8tlCqB4hLllVsZK3OyllReUZtSnmnMCpQ_KTTmuFTlyn9upSgDoVoKhQJ71b9OIyYjETjH9f_TFegZcXQGerg0t6tj7_4-TQ1p52lXtz5qAK-eEhqWw9zBZGn8AWNUb_n6_8AoHvqFs</recordid><startdate>20120530</startdate><enddate>20120530</enddate><creator>Du, Zhuo</creator><creator>Zhang, Suling</creator><creator>Zhou, Chanyuan</creator><creator>Liu, Miao</creator><creator>Li, Gongke</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120530</creationdate><title>Dynamic layer-by-layer self-assembly of multi-walled carbon nanotubes on quartz wool for on-line separation of lysozyme in egg white</title><author>Du, Zhuo ; Zhang, Suling ; Zhou, Chanyuan ; Liu, Miao ; Li, Gongke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-2ed01348262f7e591cb43676ebd6550b175d35da73c09e0ecc5f1202b96f94fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adsorption</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chickens</topic><topic>Dynamic layer-by-layer self-assembly</topic><topic>Egg White - chemistry</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Interactions. Associations</topic><topic>Intermolecular phenomena</topic><topic>Lysozyme</topic><topic>Molecular biophysics</topic><topic>Multi-walled carbon nanotubes</topic><topic>Muramidase - isolation & purification</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Osmolar Concentration</topic><topic>Quartz - chemistry</topic><topic>Quartz wool</topic><topic>Solid Phase Extraction - methods</topic><topic>Solid-phase extraction</topic><topic>Wool - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Zhuo</creatorcontrib><creatorcontrib>Zhang, Suling</creatorcontrib><creatorcontrib>Zhou, Chanyuan</creatorcontrib><creatorcontrib>Liu, Miao</creatorcontrib><creatorcontrib>Li, Gongke</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Zhuo</au><au>Zhang, Suling</au><au>Zhou, Chanyuan</au><au>Liu, Miao</au><au>Li, Gongke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic layer-by-layer self-assembly of multi-walled carbon nanotubes on quartz wool for on-line separation of lysozyme in egg white</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2012-05-30</date><risdate>2012</risdate><volume>94</volume><spage>104</spage><epage>110</epage><pages>104-110</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>The multi-walled carbon nanotubes (MWNTs) coated quartz wool (MWNTs/QW) prepared by dynamic layer-by-layer self-assembly was used as solid-phase extraction (SPE) absorbent for on-line separation and preconcentration of lysozyme in egg white. 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adsorption Analytical chemistry Animals Biological and medical sciences Chemistry Chickens Dynamic layer-by-layer self-assembly Egg White - chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Interactions. Associations Intermolecular phenomena Lysozyme Molecular biophysics Multi-walled carbon nanotubes Muramidase - isolation & purification Nanotubes, Carbon - chemistry Osmolar Concentration Quartz - chemistry Quartz wool Solid Phase Extraction - methods Solid-phase extraction Wool - chemistry |
| title | Dynamic layer-by-layer self-assembly of multi-walled carbon nanotubes on quartz wool for on-line separation of lysozyme in egg white |
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