Quantitative Approaches to The Analysis of Carbohydrate-Binding Module Function
Carbohydrate-binding modules (CBMs) are important components of carbohydrate-active enzymes. Their primary functions are to assist in substrate turnover by targeting appended catalytic modules to substrate and concentrating appended catalytic modules on the surface of substrate. Presented here are f...
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| Veröffentlicht in: | Cellulases 2012, Vol.510, p.211-231 |
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| container_title | Cellulases |
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| creator | Abbott, D. Wade Boraston, Alisdair B. |
| description | Carbohydrate-binding modules (CBMs) are important components of carbohydrate-active enzymes. Their primary functions are to assist in substrate turnover by targeting appended catalytic modules to substrate and concentrating appended catalytic modules on the surface of substrate. Presented here are four well-established methodologies for investigating and quantifying the CBM–polysaccharide binding relationship. These methods include: (1) the solid state depletion assay, (2) affinity gel electrophoresis, (3) UV difference and fluorescence spectroscopy, and (4) isothermal titration calorimetry. In addition, entropy-driven CBM–crystalline cellulose binding events and differential approaches to calculating stoichiometry with polyvalent polysaccharide ligands are also discussed. |
| doi_str_mv | 10.1016/B978-0-12-415931-0.00011-2 |
| format | Article |
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In addition, entropy-driven CBM–crystalline cellulose binding events and differential approaches to calculating stoichiometry with polyvalent polysaccharide ligands are also discussed.</description><subject>Affinity gel electrophoresis</subject><subject>Binding Sites</subject><subject>Calorimetry - methods</subject><subject>Carbohydrate Metabolism</subject><subject>Carbohydrate-binding module</subject><subject>Carbohydrates - chemistry</subject><subject>Cellulose - chemistry</subject><subject>Cellulose - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Enzymology</subject><subject>Isothermal titration calorimetry</subject><subject>Microbiology (non-medical)</subject><subject>Models, Molecular</subject><subject>Polysaccharide</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - metabolism</subject><subject>Protein Binding</subject><subject>Solid state depletion assay</subject><subject>Spectrophotometry, Ultraviolet - methods</subject><subject>Thermodynamics</subject><subject>UV difference spectroscopy</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>9780124159310</isbn><isbn>0124159311</isbn><isbn>9780124159662</isbn><isbn>0124159664</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUctuFDEQNO-swv4CGuXExcHvxzFZCCAFRUjhbHk8PaxhdrzYnkj793jZICFOLrmrStVdCF1QckkJVe-urTaYYMqwoNJyisklIYRSzJ6gdZsRyo4DpdhTtKJSaqytMc_-nXFKnqMVIVphZbR9iVbaCs6MleoMrUv50QyJ0spK8QqdMaaI0cys0N3Xxc81Vl_jA3RX-31OPmyhdDV199v2M_vpUGLp0thtfO7T9jBkXwFfx3mI8_fuSxqWCbqbZQ41pvk1ejH6qcD68T1H324-3G8-4du7j583V7c4CG4r1saO1upAR2VYIF4aGSShI5dC-8GKHqhWfuQG-h6I1oQLw6W0HkYZhLX8HL09-bbAvxYo1e1iCTBNfoa0FNfuKpnQhJlGffNIXfodDG6f487ng_t7hEbg_3lBn9LPAHPNfgpbv6-Qi7NNIKhjRLkGmur9SQVtzYcI2ZUQYQ4wxAyhuiHFluIYRLljwa5h5k5dNfynYMf4b0F8jtc</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Abbott, D. 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Wade</creatorcontrib><creatorcontrib>Boraston, Alisdair B.</creatorcontrib><collection>ProQuest Ebook Central - Book Chapters - Demo use only</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cellulases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abbott, D. 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| ispartof | Cellulases, 2012, Vol.510, p.211-231 |
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| subjects | Affinity gel electrophoresis Binding Sites Calorimetry - methods Carbohydrate Metabolism Carbohydrate-binding module Carbohydrates - chemistry Cellulose - chemistry Cellulose - metabolism Electrophoresis, Polyacrylamide Gel - methods Enzymology Isothermal titration calorimetry Microbiology (non-medical) Models, Molecular Polysaccharide Polysaccharides - chemistry Polysaccharides - metabolism Protein Binding Solid state depletion assay Spectrophotometry, Ultraviolet - methods Thermodynamics UV difference spectroscopy |
| title | Quantitative Approaches to The Analysis of Carbohydrate-Binding Module Function |
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