Isolation and Identification of CXCR4-positive Cells from Human Dental Pulp Cells
Abstract Introduction In previous studies, we found expression of stromal cell–derived factor-1α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) in human dental pulp and the SDF-1α–CXCR4 axis might play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4+ DPCs) toward the damaged sites. H...
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| Veröffentlicht in: | Journal of endodontics 2012-06, Vol.38 (6), p.791-795 |
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| container_title | Journal of endodontics |
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| creator | Jiang, Long, DDS, MDS Peng, Wei-Wei, DDS, MDS Li, Li-Fen, DDS, MDS Yang, Ya, DDS, PhD Zhu, Ya-Qin, DDS, PhD |
| description | Abstract Introduction In previous studies, we found expression of stromal cell–derived factor-1α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) in human dental pulp and the SDF-1α–CXCR4 axis might play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4+ DPCs) toward the damaged sites. However, the specific function of CXCR4+ DPCs in the injured dental pulp was still unknown. The purpose of this study was to isolate CXCR4+ DPCs from dental pulp cells in vitro to pave the way for further study of their characteristics. Methods CXCR4+ DPCs were isolated with magnetic-activated cell sorting (MACS). Freshly isolated CXCR4+ DPCs were identified by immunohistochemistry with light microscopy or confocal microscopy. Then the phenotypes CXCR4, stromal cell surface marker-1 (STRO-1), CD146, and CD34 in 3 groups (ie, CXCR4+ DPCs, CXCR4− DPCs, or non-sorted DPCs) were analyzed by flow cytometry after they were cultured and expanded in vitro. Results The results indicated the isolated subpopulation of DPCs was enriched with CXCR4+ DPCs, and the positive rates of STRO-1 and CD146 in CXCR4+ DPCs group were higher than CXCR4− DPCs or non-sorted DPCs groups ( P < .05). There was no expression of CD34 in each group. Conclusions We can isolate CXCR4+ DPCs from DPCs with MACS and identify them by immunohistochemistry and flow cytometry. |
| doi_str_mv | 10.1016/j.joen.2012.02.024 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1015094856</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S009923991200218X</els_id><sourcerecordid>1015094856</sourcerecordid><originalsourceid>FETCH-LOGICAL-c477t-15edff5aa68ab4c1d6914d523ab82a9f3b89546156d139f7cd80fb408fd61d533</originalsourceid><addsrcrecordid>eNp9kUtr3TAQRkVpaG7T_oEuipfd-FZPW4IQKO4jFwJt0hayE7IeINeWbiU7kH9fGadZZBEYEEjnG0ZnAHiH4B5B1Hwc9kO0YY8hwnu4Fn0Bdoi3vCaM0ZdgB6EQNSZCnILXOQ8QopaQ9hU4xZgJhhDdgetDjqOafQyVCqY6GBtm77zerqKrutvuhtbHmP3s72zV2XHMlUtxqi6XSYXqcwmosfqxjMft8Q04cWrM9u3DeQZ-f_3yq7usr75_O3SfrmpN23auEbPGOaZUw1VPNTKNQNQwTFTPsRKO9Fww2iDWGESEa7Xh0PUUcmcaZBghZ-DD1veY4t_F5llOPusygQo2LlkWRQwKyllTULyhOsWck3XymPyk0n2BVq6Rg1xVylWlhGvREnr_0H_pJ2seI__dFeB8A2z55Z23SWbtbdDW-GT1LE30z_e_eBLXow_F_PjH3ts8xCWF4k8imUtA_lyXue4SYQgx4rfkH2TTmPQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1015094856</pqid></control><display><type>article</type><title>Isolation and Identification of CXCR4-positive Cells from Human Dental Pulp Cells</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Jiang, Long, DDS, MDS ; Peng, Wei-Wei, DDS, MDS ; Li, Li-Fen, DDS, MDS ; Yang, Ya, DDS, PhD ; Zhu, Ya-Qin, DDS, PhD</creator><creatorcontrib>Jiang, Long, DDS, MDS ; Peng, Wei-Wei, DDS, MDS ; Li, Li-Fen, DDS, MDS ; Yang, Ya, DDS, PhD ; Zhu, Ya-Qin, DDS, PhD</creatorcontrib><description>Abstract Introduction In previous studies, we found expression of stromal cell–derived factor-1α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) in human dental pulp and the SDF-1α–CXCR4 axis might play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4+ DPCs) toward the damaged sites. However, the specific function of CXCR4+ DPCs in the injured dental pulp was still unknown. The purpose of this study was to isolate CXCR4+ DPCs from dental pulp cells in vitro to pave the way for further study of their characteristics. Methods CXCR4+ DPCs were isolated with magnetic-activated cell sorting (MACS). Freshly isolated CXCR4+ DPCs were identified by immunohistochemistry with light microscopy or confocal microscopy. Then the phenotypes CXCR4, stromal cell surface marker-1 (STRO-1), CD146, and CD34 in 3 groups (ie, CXCR4+ DPCs, CXCR4− DPCs, or non-sorted DPCs) were analyzed by flow cytometry after they were cultured and expanded in vitro. Results The results indicated the isolated subpopulation of DPCs was enriched with CXCR4+ DPCs, and the positive rates of STRO-1 and CD146 in CXCR4+ DPCs group were higher than CXCR4− DPCs or non-sorted DPCs groups ( P < .05). There was no expression of CD34 in each group. Conclusions We can isolate CXCR4+ DPCs from DPCs with MACS and identify them by immunohistochemistry and flow cytometry.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2012.02.024</identifier><identifier>PMID: 22595114</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Analysis of Variance ; Cell Culture Techniques ; Cell Separation - methods ; Chemokine CXCL12 - metabolism ; CXCR4 ; Dental Pulp - cytology ; Dental Pulp - metabolism ; dental pulp stem cells ; Dentistry ; Endocrinology & Metabolism ; Flow Cytometry ; Fluorescent Antibody Technique ; Humans ; Magnetic Fields ; magnetic-activated cell sorting ; Mesenchymal Stromal Cells - chemistry ; Mesenchymal Stromal Cells - metabolism ; Primary Cell Culture ; Receptors, CXCR4 - analysis ; Receptors, CXCR4 - isolation & purification ; Receptors, CXCR4 - metabolism ; Young Adult</subject><ispartof>Journal of endodontics, 2012-06, Vol.38 (6), p.791-795</ispartof><rights>American Association of Endodontists</rights><rights>2012 American Association of Endodontists</rights><rights>Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-15edff5aa68ab4c1d6914d523ab82a9f3b89546156d139f7cd80fb408fd61d533</citedby><cites>FETCH-LOGICAL-c477t-15edff5aa68ab4c1d6914d523ab82a9f3b89546156d139f7cd80fb408fd61d533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.joen.2012.02.024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22595114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Long, DDS, MDS</creatorcontrib><creatorcontrib>Peng, Wei-Wei, DDS, MDS</creatorcontrib><creatorcontrib>Li, Li-Fen, DDS, MDS</creatorcontrib><creatorcontrib>Yang, Ya, DDS, PhD</creatorcontrib><creatorcontrib>Zhu, Ya-Qin, DDS, PhD</creatorcontrib><title>Isolation and Identification of CXCR4-positive Cells from Human Dental Pulp Cells</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract Introduction In previous studies, we found expression of stromal cell–derived factor-1α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) in human dental pulp and the SDF-1α–CXCR4 axis might play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4+ DPCs) toward the damaged sites. However, the specific function of CXCR4+ DPCs in the injured dental pulp was still unknown. The purpose of this study was to isolate CXCR4+ DPCs from dental pulp cells in vitro to pave the way for further study of their characteristics. Methods CXCR4+ DPCs were isolated with magnetic-activated cell sorting (MACS). Freshly isolated CXCR4+ DPCs were identified by immunohistochemistry with light microscopy or confocal microscopy. Then the phenotypes CXCR4, stromal cell surface marker-1 (STRO-1), CD146, and CD34 in 3 groups (ie, CXCR4+ DPCs, CXCR4− DPCs, or non-sorted DPCs) were analyzed by flow cytometry after they were cultured and expanded in vitro. Results The results indicated the isolated subpopulation of DPCs was enriched with CXCR4+ DPCs, and the positive rates of STRO-1 and CD146 in CXCR4+ DPCs group were higher than CXCR4− DPCs or non-sorted DPCs groups ( P < .05). There was no expression of CD34 in each group. Conclusions We can isolate CXCR4+ DPCs from DPCs with MACS and identify them by immunohistochemistry and flow cytometry.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Analysis of Variance</subject><subject>Cell Culture Techniques</subject><subject>Cell Separation - methods</subject><subject>Chemokine CXCL12 - metabolism</subject><subject>CXCR4</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - metabolism</subject><subject>dental pulp stem cells</subject><subject>Dentistry</subject><subject>Endocrinology & Metabolism</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Magnetic Fields</subject><subject>magnetic-activated cell sorting</subject><subject>Mesenchymal Stromal Cells - chemistry</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Primary Cell Culture</subject><subject>Receptors, CXCR4 - analysis</subject><subject>Receptors, CXCR4 - isolation & purification</subject><subject>Receptors, CXCR4 - metabolism</subject><subject>Young Adult</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtr3TAQRkVpaG7T_oEuipfd-FZPW4IQKO4jFwJt0hayE7IeINeWbiU7kH9fGadZZBEYEEjnG0ZnAHiH4B5B1Hwc9kO0YY8hwnu4Fn0Bdoi3vCaM0ZdgB6EQNSZCnILXOQ8QopaQ9hU4xZgJhhDdgetDjqOafQyVCqY6GBtm77zerqKrutvuhtbHmP3s72zV2XHMlUtxqi6XSYXqcwmosfqxjMft8Q04cWrM9u3DeQZ-f_3yq7usr75_O3SfrmpN23auEbPGOaZUw1VPNTKNQNQwTFTPsRKO9Fww2iDWGESEa7Xh0PUUcmcaZBghZ-DD1veY4t_F5llOPusygQo2LlkWRQwKyllTULyhOsWck3XymPyk0n2BVq6Rg1xVylWlhGvREnr_0H_pJ2seI__dFeB8A2z55Z23SWbtbdDW-GT1LE30z_e_eBLXow_F_PjH3ts8xCWF4k8imUtA_lyXue4SYQgx4rfkH2TTmPQ</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Jiang, Long, DDS, MDS</creator><creator>Peng, Wei-Wei, DDS, MDS</creator><creator>Li, Li-Fen, DDS, MDS</creator><creator>Yang, Ya, DDS, PhD</creator><creator>Zhu, Ya-Qin, DDS, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120601</creationdate><title>Isolation and Identification of CXCR4-positive Cells from Human Dental Pulp Cells</title><author>Jiang, Long, DDS, MDS ; Peng, Wei-Wei, DDS, MDS ; Li, Li-Fen, DDS, MDS ; Yang, Ya, DDS, PhD ; Zhu, Ya-Qin, DDS, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-15edff5aa68ab4c1d6914d523ab82a9f3b89546156d139f7cd80fb408fd61d533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Analysis of Variance</topic><topic>Cell Culture Techniques</topic><topic>Cell Separation - methods</topic><topic>Chemokine CXCL12 - metabolism</topic><topic>CXCR4</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - metabolism</topic><topic>dental pulp stem cells</topic><topic>Dentistry</topic><topic>Endocrinology & Metabolism</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Magnetic Fields</topic><topic>magnetic-activated cell sorting</topic><topic>Mesenchymal Stromal Cells - chemistry</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Primary Cell Culture</topic><topic>Receptors, CXCR4 - analysis</topic><topic>Receptors, CXCR4 - isolation & purification</topic><topic>Receptors, CXCR4 - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Long, DDS, MDS</creatorcontrib><creatorcontrib>Peng, Wei-Wei, DDS, MDS</creatorcontrib><creatorcontrib>Li, Li-Fen, DDS, MDS</creatorcontrib><creatorcontrib>Yang, Ya, DDS, PhD</creatorcontrib><creatorcontrib>Zhu, Ya-Qin, DDS, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Long, DDS, MDS</au><au>Peng, Wei-Wei, DDS, MDS</au><au>Li, Li-Fen, DDS, MDS</au><au>Yang, Ya, DDS, PhD</au><au>Zhu, Ya-Qin, DDS, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Identification of CXCR4-positive Cells from Human Dental Pulp Cells</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>38</volume><issue>6</issue><spage>791</spage><epage>795</epage><pages>791-795</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract Introduction In previous studies, we found expression of stromal cell–derived factor-1α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) in human dental pulp and the SDF-1α–CXCR4 axis might play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4+ DPCs) toward the damaged sites. However, the specific function of CXCR4+ DPCs in the injured dental pulp was still unknown. The purpose of this study was to isolate CXCR4+ DPCs from dental pulp cells in vitro to pave the way for further study of their characteristics. Methods CXCR4+ DPCs were isolated with magnetic-activated cell sorting (MACS). Freshly isolated CXCR4+ DPCs were identified by immunohistochemistry with light microscopy or confocal microscopy. Then the phenotypes CXCR4, stromal cell surface marker-1 (STRO-1), CD146, and CD34 in 3 groups (ie, CXCR4+ DPCs, CXCR4− DPCs, or non-sorted DPCs) were analyzed by flow cytometry after they were cultured and expanded in vitro. Results The results indicated the isolated subpopulation of DPCs was enriched with CXCR4+ DPCs, and the positive rates of STRO-1 and CD146 in CXCR4+ DPCs group were higher than CXCR4− DPCs or non-sorted DPCs groups ( P < .05). There was no expression of CD34 in each group. Conclusions We can isolate CXCR4+ DPCs from DPCs with MACS and identify them by immunohistochemistry and flow cytometry.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22595114</pmid><doi>10.1016/j.joen.2012.02.024</doi><tpages>5</tpages></addata></record> |
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| subjects | Adolescent Adult Analysis of Variance Cell Culture Techniques Cell Separation - methods Chemokine CXCL12 - metabolism CXCR4 Dental Pulp - cytology Dental Pulp - metabolism dental pulp stem cells Dentistry Endocrinology & Metabolism Flow Cytometry Fluorescent Antibody Technique Humans Magnetic Fields magnetic-activated cell sorting Mesenchymal Stromal Cells - chemistry Mesenchymal Stromal Cells - metabolism Primary Cell Culture Receptors, CXCR4 - analysis Receptors, CXCR4 - isolation & purification Receptors, CXCR4 - metabolism Young Adult |
| title | Isolation and Identification of CXCR4-positive Cells from Human Dental Pulp Cells |
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