Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro
By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. The kinetics of neuronal...
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| Veröffentlicht in: | Biointerphases 2012-12, Vol.7 (1-4), p.29-29 |
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| creator | Sun, Yi Huang, Zhuo Liu, Wenwen Yang, Kaixuan Sun, Kang Xing, Shige Wang, Dong Zhang, Wei Jiang, Xingyu |
| description | By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. The kinetics of neuronal adhesion on poly-lysine-coated surfaces is much faster than that on laminin and Matrigel-coated surfaces, and the distribution of adhesion is more homogenous on poly-lysine. Matrigel and laminin, on the other hand, facilitate neuritogenesis more than poly-lysine does. Eventually, on Matrigel-coated surfaces of self-assembled monolayers, neurons tend to undergo somata clustering and neurite fasciculation. By replacing coating proteins with cerebral astrocytes, and patterning neurons on astrocytes through self-assembled monolayers, microfluidics and micro-contact printing, we found that astrocyte promotes soma adhesion and astrocyte processes guide neurites. There, astrocytes could be a versatile substrate in engineering neuronal networks in vitro. Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy. |
| doi_str_mv | 10.1007/s13758-012-0029-7 |
| format | Article |
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Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy.</description><identifier>ISSN: 1934-8630</identifier><identifier>EISSN: 1559-4106</identifier><identifier>DOI: 10.1007/s13758-012-0029-7</identifier><identifier>PMID: 22589072</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Biological and Medical Physics ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biophysics ; Cell Adhesion ; Cell Proliferation ; Coated Materials, Biocompatible - chemistry ; Collagen - chemistry ; Drug Combinations ; Laminin - chemistry ; Materials Science ; Neurons - physiology ; Physical Chemistry ; Polylysine - chemistry ; Proteoglycans - chemistry ; Rats ; Rats, Sprague-Dawley ; Surface and Interface Science ; Thin Films ; Tissue Engineering - methods</subject><ispartof>Biointerphases, 2012-12, Vol.7 (1-4), p.29-29</ispartof><rights>The Author(s) 2012. This article is published under license to BioMed Central Ltd. 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The kinetics of neuronal adhesion on poly-lysine-coated surfaces is much faster than that on laminin and Matrigel-coated surfaces, and the distribution of adhesion is more homogenous on poly-lysine. Matrigel and laminin, on the other hand, facilitate neuritogenesis more than poly-lysine does. Eventually, on Matrigel-coated surfaces of self-assembled monolayers, neurons tend to undergo somata clustering and neurite fasciculation. By replacing coating proteins with cerebral astrocytes, and patterning neurons on astrocytes through self-assembled monolayers, microfluidics and micro-contact printing, we found that astrocyte promotes soma adhesion and astrocyte processes guide neurites. There, astrocytes could be a versatile substrate in engineering neuronal networks in vitro. Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy.</description><subject>Animals</subject><subject>Biological and Medical Physics</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biophysics</subject><subject>Cell Adhesion</subject><subject>Cell Proliferation</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Collagen - chemistry</subject><subject>Drug Combinations</subject><subject>Laminin - chemistry</subject><subject>Materials Science</subject><subject>Neurons - physiology</subject><subject>Physical Chemistry</subject><subject>Polylysine - chemistry</subject><subject>Proteoglycans - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Surface and Interface Science</subject><subject>Thin Films</subject><subject>Tissue Engineering - methods</subject><issn>1934-8630</issn><issn>1559-4106</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlZ_gBfJ0ctqZrPZJEcpfhSLCtVeQ5pOytZ2tya7SP-9Ka0ePc3APO_L8BByCewGGJO3EbgUKmOQZ4zlOpNHpA9C6KwAVh6nXfMiUyVnPXIW45KxQoiSn5Jengulmcz7ZDrpgrcO6bCxbVUvqI3U0mfc0jcb7BpbDLSq6X29qGrEsCNesAtNbVdpab-b8Eknbehc2wWMdFTTadWG5pyceLuKeHGYA_LxcP8-fMrGr4-j4d04cyLnbQbzQvi5UsoBE6BLdFKCU4Jprb0rcg5-PvM8vepRKRRWp5NgcuYd167kfECu972b0Hx1GFuzrqLD1crW2HTRAIMCgEkQCYU96kITY0BvNqFa27BNkNnpNHudJuk0O51GpszVob6brXH-l_j1l4B8D8TNzg0Gs2y6kOTEf1p_AAjVfwg</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Sun, Yi</creator><creator>Huang, Zhuo</creator><creator>Liu, Wenwen</creator><creator>Yang, Kaixuan</creator><creator>Sun, Kang</creator><creator>Xing, Shige</creator><creator>Wang, Dong</creator><creator>Zhang, Wei</creator><creator>Jiang, Xingyu</creator><general>Springer Berlin Heidelberg</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121201</creationdate><title>Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro</title><author>Sun, Yi ; Huang, Zhuo ; Liu, Wenwen ; Yang, Kaixuan ; Sun, Kang ; Xing, Shige ; Wang, Dong ; Zhang, Wei ; Jiang, Xingyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-1d45fd888c105196ec771c850999fc4231fdbf3907fe88e5a9509507bfc39c633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biological and Medical Physics</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biophysics</topic><topic>Cell Adhesion</topic><topic>Cell Proliferation</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Collagen - chemistry</topic><topic>Drug Combinations</topic><topic>Laminin - chemistry</topic><topic>Materials Science</topic><topic>Neurons - physiology</topic><topic>Physical Chemistry</topic><topic>Polylysine - chemistry</topic><topic>Proteoglycans - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Surface and Interface Science</topic><topic>Thin Films</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Yi</creatorcontrib><creatorcontrib>Huang, Zhuo</creatorcontrib><creatorcontrib>Liu, Wenwen</creatorcontrib><creatorcontrib>Yang, Kaixuan</creatorcontrib><creatorcontrib>Sun, Kang</creatorcontrib><creatorcontrib>Xing, Shige</creatorcontrib><creatorcontrib>Wang, Dong</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Jiang, Xingyu</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biointerphases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Yi</au><au>Huang, Zhuo</au><au>Liu, Wenwen</au><au>Yang, Kaixuan</au><au>Sun, Kang</au><au>Xing, Shige</au><au>Wang, Dong</au><au>Zhang, Wei</au><au>Jiang, Xingyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro</atitle><jtitle>Biointerphases</jtitle><stitle>Biointerphases</stitle><addtitle>Biointerphases</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>7</volume><issue>1-4</issue><spage>29</spage><epage>29</epage><pages>29-29</pages><issn>1934-8630</issn><eissn>1559-4106</eissn><abstract>By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. 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| subjects | Animals Biological and Medical Physics Biomaterials Biomedical Engineering and Bioengineering Biophysics Cell Adhesion Cell Proliferation Coated Materials, Biocompatible - chemistry Collagen - chemistry Drug Combinations Laminin - chemistry Materials Science Neurons - physiology Physical Chemistry Polylysine - chemistry Proteoglycans - chemistry Rats Rats, Sprague-Dawley Surface and Interface Science Thin Films Tissue Engineering - methods |
| title | Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro |
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