Isolation of the opdE gene that encodes for a new hydrolase of Enterobacter sp. capable of degrading organophosphorus pesticides
Microbial enzymes that can hydrolyze organophosphorus compounds have been isolated, identified and characterized from different microbial species in order to use them in biodegradation of organophosphorus compounds. We isolated a bacterial strain Cons002 from an agricultural soil bacterial consortiu...
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| Veröffentlicht in: | Biodegradation (Dordrecht) 2012-06, Vol.23 (3), p.387-397 |
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| creator | Chino-Flores, Concepción Dantán-González, Edgar Vázquez-Ramos, Alejandra Tinoco-Valencia, Raunel Díaz-Méndez, Rafael Sánchez-Salinas, Enrique Castrejón-Godínez, Ma. Luisa Ramos-Quintana, Fernando Ortiz-Hernández, Ma. Laura |
| description | Microbial enzymes that can hydrolyze organophosphorus compounds have been isolated, identified and characterized from different microbial species in order to use them in biodegradation of organophosphorus compounds. We isolated a bacterial strain Cons002 from an agricultural soil bacterial consortium, which can hydrolyze methyl-parathion (MP) and other organophosphate pesticides. HPLC analysis showed that strain Cons002 is capable of degrading pesticides MP, parathion and phorate. Pulsed-field gel electrophoresis and 16S rRNA amplification were performed for strain characterization and identification, respectively, showing that the strain Cons002 is related to the genus
Enterobacter
sp. which has a single chromosome of 4.6 Mb and has no plasmids. Genomic library was constructed from DNA of
Enterobacter
sp. Cons002. A gene called
opdE
(
O
rganophosphate
D
egradation from
E
nterobacter
) consists of 753 bp and encodes a protein of 25 kDa, which was isolated using activity methods. This gene
opdE
had no similarity to any genes reported to degrade organophosphates. When kanamycin-resistance cassette was placed in the gene
opdE
, hydrolase activity was suppressed and
Enterobacter
sp. Cons002 had no growth with MP as a nutrients source. |
| doi_str_mv | 10.1007/s10532-011-9517-6 |
| format | Article |
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Enterobacter
sp. which has a single chromosome of 4.6 Mb and has no plasmids. Genomic library was constructed from DNA of
Enterobacter
sp. Cons002. A gene called
opdE
(
O
rganophosphate
D
egradation from
E
nterobacter
) consists of 753 bp and encodes a protein of 25 kDa, which was isolated using activity methods. This gene
opdE
had no similarity to any genes reported to degrade organophosphates. When kanamycin-resistance cassette was placed in the gene
opdE
, hydrolase activity was suppressed and
Enterobacter
sp. Cons002 had no growth with MP as a nutrients source.</description><identifier>ISSN: 0923-9820</identifier><identifier>EISSN: 1572-9729</identifier><identifier>DOI: 10.1007/s10532-011-9517-6</identifier><identifier>PMID: 22065283</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agricultural land ; Analysis ; Aquatic Pollution ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biodegradation ; Biodegradation of pollutants ; Biodegradation, Environmental ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Consortia ; Enterobacter ; Enterobacter - enzymology ; Enterobacter - genetics ; Enterobacter - isolation & purification ; Enterobacter - metabolism ; Environment and pollution ; Enzyme Stability ; Enzymes ; Ethylenediaminetetraacetic acid ; Fundamental and applied biological sciences. Psychology ; Genes ; Genomic libraries ; Geochemistry ; Hydrolases ; Hydrolases - chemistry ; Hydrolases - genetics ; Hydrolases - metabolism ; Industrial applications and implications. Economical aspects ; Insecticides ; Kinetics ; Life Sciences ; Liquid chromatography ; Methyl parathion ; Microbiology ; Molecular Sequence Data ; Organophosphates ; Organophosphorus compounds ; Organophosphorus Compounds - metabolism ; Organophosphorus pesticides ; Original Paper ; Parathion ; Pesticides ; Pesticides - metabolism ; Phylogeny ; Political aspects ; RNA ; Soil Microbiology ; Soil Science & Conservation ; Terrestrial Pollution ; Waste Management/Waste Technology ; Waste Water Technology ; Water Management ; Water Pollution Control</subject><ispartof>Biodegradation (Dordrecht), 2012-06, Vol.23 (3), p.387-397</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2012 Springer</rights><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-dd5a9aba2aa7e6f8b3943a9d9229163df1ac0559ad30c74edeac53404471e25d3</citedby><cites>FETCH-LOGICAL-c474t-dd5a9aba2aa7e6f8b3943a9d9229163df1ac0559ad30c74edeac53404471e25d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10532-011-9517-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10532-011-9517-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25841069$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22065283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chino-Flores, Concepción</creatorcontrib><creatorcontrib>Dantán-González, Edgar</creatorcontrib><creatorcontrib>Vázquez-Ramos, Alejandra</creatorcontrib><creatorcontrib>Tinoco-Valencia, Raunel</creatorcontrib><creatorcontrib>Díaz-Méndez, Rafael</creatorcontrib><creatorcontrib>Sánchez-Salinas, Enrique</creatorcontrib><creatorcontrib>Castrejón-Godínez, Ma. Luisa</creatorcontrib><creatorcontrib>Ramos-Quintana, Fernando</creatorcontrib><creatorcontrib>Ortiz-Hernández, Ma. Laura</creatorcontrib><title>Isolation of the opdE gene that encodes for a new hydrolase of Enterobacter sp. capable of degrading organophosphorus pesticides</title><title>Biodegradation (Dordrecht)</title><addtitle>Biodegradation</addtitle><addtitle>Biodegradation</addtitle><description>Microbial enzymes that can hydrolyze organophosphorus compounds have been isolated, identified and characterized from different microbial species in order to use them in biodegradation of organophosphorus compounds. We isolated a bacterial strain Cons002 from an agricultural soil bacterial consortium, which can hydrolyze methyl-parathion (MP) and other organophosphate pesticides. HPLC analysis showed that strain Cons002 is capable of degrading pesticides MP, parathion and phorate. Pulsed-field gel electrophoresis and 16S rRNA amplification were performed for strain characterization and identification, respectively, showing that the strain Cons002 is related to the genus
Enterobacter
sp. which has a single chromosome of 4.6 Mb and has no plasmids. Genomic library was constructed from DNA of
Enterobacter
sp. Cons002. A gene called
opdE
(
O
rganophosphate
D
egradation from
E
nterobacter
) consists of 753 bp and encodes a protein of 25 kDa, which was isolated using activity methods. This gene
opdE
had no similarity to any genes reported to degrade organophosphates. When kanamycin-resistance cassette was placed in the gene
opdE
, hydrolase activity was suppressed and
Enterobacter
sp. Cons002 had no growth with MP as a nutrients source.</description><subject>Agricultural land</subject><subject>Analysis</subject><subject>Aquatic Pollution</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biodegradation</subject><subject>Biodegradation of pollutants</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Consortia</subject><subject>Enterobacter</subject><subject>Enterobacter - enzymology</subject><subject>Enterobacter - genetics</subject><subject>Enterobacter - isolation & purification</subject><subject>Enterobacter - metabolism</subject><subject>Environment and pollution</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genomic libraries</subject><subject>Geochemistry</subject><subject>Hydrolases</subject><subject>Hydrolases - chemistry</subject><subject>Hydrolases - genetics</subject><subject>Hydrolases - metabolism</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Insecticides</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Methyl parathion</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Organophosphates</subject><subject>Organophosphorus compounds</subject><subject>Organophosphorus Compounds - metabolism</subject><subject>Organophosphorus pesticides</subject><subject>Original Paper</subject><subject>Parathion</subject><subject>Pesticides</subject><subject>Pesticides - metabolism</subject><subject>Phylogeny</subject><subject>Political aspects</subject><subject>RNA</subject><subject>Soil Microbiology</subject><subject>Soil Science & Conservation</subject><subject>Terrestrial Pollution</subject><subject>Waste Management/Waste Technology</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><issn>0923-9820</issn><issn>1572-9729</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkV2L1TAQhoso7nH1B3gjARG86XGSNk1zuSxHXVjwRq_LNJn2dOlJatIie7c_3dQePxAECSEk87zvzGSy7CWHPQdQ7yIHWYgcOM-15CqvHmU7LpXItRL6cbYDLYpc1wIusmcx3gGAViCeZhdCQCVFXeyyh5voR5wH75jv2Hwk5id7YD05SjecGTnjLUXW-cCQOfrGjvc2JE2kVXFwMwXfokkHi9OeGZywHX_ELPUB7eB65kOPzk9HH9MOS2QTxXkwQzJ-nj3pcIz04nxeZl_eHz5ff8xvP324ub66zU2pyjm3VqLGFgWioqqr20KXBWqrhdC8KmzH0YCUGm0BRpVkCY0sSihLxUlIW1xmbzffKfivS0rfnIZoaBzRkV9iw4GXHJTU8B8oaFnIEqqEvv4LvfNLcKmRlaprLbSUidpvVI8jNYPr_BzQpGXpNBjvqBvS-5XiqRkJUiQB3wQm-BgDdc0UhhOG--S6GqtmG32TRt-so2_WUl6dS1naE9lfip-zTsCbM4DR4NgFdGaIvzlZpw-odOLExsUUcj2FP3v6V_bvHOPGBQ</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Chino-Flores, Concepción</creator><creator>Dantán-González, Edgar</creator><creator>Vázquez-Ramos, Alejandra</creator><creator>Tinoco-Valencia, Raunel</creator><creator>Díaz-Méndez, Rafael</creator><creator>Sánchez-Salinas, Enrique</creator><creator>Castrejón-Godínez, Ma. 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Luisa ; Ramos-Quintana, Fernando ; Ortiz-Hernández, Ma. Laura</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-dd5a9aba2aa7e6f8b3943a9d9229163df1ac0559ad30c74edeac53404471e25d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agricultural land</topic><topic>Analysis</topic><topic>Aquatic Pollution</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biodegradation</topic><topic>Biodegradation of pollutants</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Consortia</topic><topic>Enterobacter</topic><topic>Enterobacter - enzymology</topic><topic>Enterobacter - genetics</topic><topic>Enterobacter - isolation & purification</topic><topic>Enterobacter - metabolism</topic><topic>Environment and pollution</topic><topic>Enzyme Stability</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genomic libraries</topic><topic>Geochemistry</topic><topic>Hydrolases</topic><topic>Hydrolases - chemistry</topic><topic>Hydrolases - genetics</topic><topic>Hydrolases - metabolism</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Insecticides</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Methyl parathion</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Organophosphates</topic><topic>Organophosphorus compounds</topic><topic>Organophosphorus Compounds - metabolism</topic><topic>Organophosphorus pesticides</topic><topic>Original Paper</topic><topic>Parathion</topic><topic>Pesticides</topic><topic>Pesticides - metabolism</topic><topic>Phylogeny</topic><topic>Political aspects</topic><topic>RNA</topic><topic>Soil Microbiology</topic><topic>Soil Science & Conservation</topic><topic>Terrestrial Pollution</topic><topic>Waste Management/Waste Technology</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chino-Flores, Concepción</creatorcontrib><creatorcontrib>Dantán-González, Edgar</creatorcontrib><creatorcontrib>Vázquez-Ramos, Alejandra</creatorcontrib><creatorcontrib>Tinoco-Valencia, Raunel</creatorcontrib><creatorcontrib>Díaz-Méndez, Rafael</creatorcontrib><creatorcontrib>Sánchez-Salinas, Enrique</creatorcontrib><creatorcontrib>Castrejón-Godínez, Ma. 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Luisa</au><au>Ramos-Quintana, Fernando</au><au>Ortiz-Hernández, Ma. Laura</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of the opdE gene that encodes for a new hydrolase of Enterobacter sp. capable of degrading organophosphorus pesticides</atitle><jtitle>Biodegradation (Dordrecht)</jtitle><stitle>Biodegradation</stitle><addtitle>Biodegradation</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>23</volume><issue>3</issue><spage>387</spage><epage>397</epage><pages>387-397</pages><issn>0923-9820</issn><eissn>1572-9729</eissn><abstract>Microbial enzymes that can hydrolyze organophosphorus compounds have been isolated, identified and characterized from different microbial species in order to use them in biodegradation of organophosphorus compounds. We isolated a bacterial strain Cons002 from an agricultural soil bacterial consortium, which can hydrolyze methyl-parathion (MP) and other organophosphate pesticides. HPLC analysis showed that strain Cons002 is capable of degrading pesticides MP, parathion and phorate. Pulsed-field gel electrophoresis and 16S rRNA amplification were performed for strain characterization and identification, respectively, showing that the strain Cons002 is related to the genus
Enterobacter
sp. which has a single chromosome of 4.6 Mb and has no plasmids. Genomic library was constructed from DNA of
Enterobacter
sp. Cons002. A gene called
opdE
(
O
rganophosphate
D
egradation from
E
nterobacter
) consists of 753 bp and encodes a protein of 25 kDa, which was isolated using activity methods. This gene
opdE
had no similarity to any genes reported to degrade organophosphates. When kanamycin-resistance cassette was placed in the gene
opdE
, hydrolase activity was suppressed and
Enterobacter
sp. Cons002 had no growth with MP as a nutrients source.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>22065283</pmid><doi>10.1007/s10532-011-9517-6</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0923-9820 |
| ispartof | Biodegradation (Dordrecht), 2012-06, Vol.23 (3), p.387-397 |
| issn | 0923-9820 1572-9729 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1014107590 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Agricultural land Analysis Aquatic Pollution Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Biodegradation Biodegradation of pollutants Biodegradation, Environmental Biological and medical sciences Biomedical and Life Sciences Biotechnology Consortia Enterobacter Enterobacter - enzymology Enterobacter - genetics Enterobacter - isolation & purification Enterobacter - metabolism Environment and pollution Enzyme Stability Enzymes Ethylenediaminetetraacetic acid Fundamental and applied biological sciences. Psychology Genes Genomic libraries Geochemistry Hydrolases Hydrolases - chemistry Hydrolases - genetics Hydrolases - metabolism Industrial applications and implications. Economical aspects Insecticides Kinetics Life Sciences Liquid chromatography Methyl parathion Microbiology Molecular Sequence Data Organophosphates Organophosphorus compounds Organophosphorus Compounds - metabolism Organophosphorus pesticides Original Paper Parathion Pesticides Pesticides - metabolism Phylogeny Political aspects RNA Soil Microbiology Soil Science & Conservation Terrestrial Pollution Waste Management/Waste Technology Waste Water Technology Water Management Water Pollution Control |
| title | Isolation of the opdE gene that encodes for a new hydrolase of Enterobacter sp. capable of degrading organophosphorus pesticides |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T04%3A19%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20of%20the%20opdE%20gene%20that%20encodes%20for%20a%20new%20hydrolase%20of%20Enterobacter%20sp.%20capable%20of%20degrading%20organophosphorus%20pesticides&rft.jtitle=Biodegradation%20(Dordrecht)&rft.au=Chino-Flores,%20Concepci%C3%B3n&rft.date=2012-06-01&rft.volume=23&rft.issue=3&rft.spage=387&rft.epage=397&rft.pages=387-397&rft.issn=0923-9820&rft.eissn=1572-9729&rft_id=info:doi/10.1007/s10532-011-9517-6&rft_dat=%3Cgale_proqu%3EA712295052%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1008892955&rft_id=info:pmid/22065283&rft_galeid=A712295052&rfr_iscdi=true |