Highly Sensitive Multiple microRNA Detection Based on Fluorescence Quenching of Graphene Oxide and Isothermal Strand-Displacement Polymerase Reaction
A simple, highly sensitive, and selective multiple microRNA (miRNA) detection method based on the graphene oxide (GO) fluorescence quenching and isothermal strand-displacement polymerase reaction (ISDPR) was proposed. The capability to discriminate ssDNA and double-stranded nucleic acid structure co...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2012-05, Vol.84 (10), p.4587-4593 |
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| creator | Dong, Haifeng Zhang, Jing Ju, Huangxian Lu, Huiting Wang, Shiyan Jin, Shi Hao, Kaihong Du, Hongwu Zhang, Xueji |
| description | A simple, highly sensitive, and selective multiple microRNA (miRNA) detection method based on the graphene oxide (GO) fluorescence quenching and isothermal strand-displacement polymerase reaction (ISDPR) was proposed. The capability to discriminate ssDNA and double-stranded nucleic acid structure coupled with the extraordinary fluorescence quenching of GO on multiple organic dye allows the proposed strategy to simultaneously and selectively detect several miRNA labeled with different dyes in the same solution, while the ISDPR amplification endows the detection method with high sensitivity. The strong interaction between ssDNA and GO led to the fluorescent ssDNA probe exhibiting minimal background fluorescence. Upon the recognition of specific target miRNA, an ISDPR was triggered to produce numerous massive specific DNA-miRNA duplex helixes, and a strong emission was observed due to the weak interaction between the DNA-miRNA duplex helix and GO. A miRNA biosensor down to 2.1 fM with a linear range of 4 orders of magnitude was obtained. Furthermore, the large planar surface of GO allows simultaneous quenching of several DNA probes with different dyes and produces a multiple biosensing platform with high sensitivity and selectivity, which has promising application in profiling the pattern of miRNA expression and biomedical research. |
| doi_str_mv | 10.1021/ac300721u |
| format | Article |
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The capability to discriminate ssDNA and double-stranded nucleic acid structure coupled with the extraordinary fluorescence quenching of GO on multiple organic dye allows the proposed strategy to simultaneously and selectively detect several miRNA labeled with different dyes in the same solution, while the ISDPR amplification endows the detection method with high sensitivity. The strong interaction between ssDNA and GO led to the fluorescent ssDNA probe exhibiting minimal background fluorescence. Upon the recognition of specific target miRNA, an ISDPR was triggered to produce numerous massive specific DNA-miRNA duplex helixes, and a strong emission was observed due to the weak interaction between the DNA-miRNA duplex helix and GO. A miRNA biosensor down to 2.1 fM with a linear range of 4 orders of magnitude was obtained. Furthermore, the large planar surface of GO allows simultaneous quenching of several DNA probes with different dyes and produces a multiple biosensing platform with high sensitivity and selectivity, which has promising application in profiling the pattern of miRNA expression and biomedical research.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac300721u</identifier><identifier>PMID: 22510208</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Biochemistry ; Biological and medical sciences ; Biosensors ; Biotechnology ; Chemistry ; Exact sciences and technology ; Fluorescent Dyes - chemistry ; Fundamental and applied biological sciences. Psychology ; Gene expression ; General, instrumentation ; Graphite - chemistry ; Luminescence quenching ; Methods. Procedures. Technologies ; MicroRNAs - analysis ; Nucleic Acid Amplification Techniques - instrumentation ; Nucleic Acid Amplification Techniques - methods ; Oxides - chemistry ; Polymerase chain reaction ; Ribonucleic acid ; RNA ; Spectrometric and optical methods ; Spectrometry, Fluorescence ; Various methods and equipments</subject><ispartof>Analytical chemistry (Washington), 2012-05, Vol.84 (10), p.4587-4593</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society May 15, 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a439t-ef56dbdcad2e7a36a2f7ffc7f0eab51eefaad29b9e5e360d15f1b0071386b0a33</citedby><cites>FETCH-LOGICAL-a439t-ef56dbdcad2e7a36a2f7ffc7f0eab51eefaad29b9e5e360d15f1b0071386b0a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac300721u$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac300721u$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56717,56767</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25901142$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22510208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Haifeng</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Ju, Huangxian</creatorcontrib><creatorcontrib>Lu, Huiting</creatorcontrib><creatorcontrib>Wang, Shiyan</creatorcontrib><creatorcontrib>Jin, Shi</creatorcontrib><creatorcontrib>Hao, Kaihong</creatorcontrib><creatorcontrib>Du, Hongwu</creatorcontrib><creatorcontrib>Zhang, Xueji</creatorcontrib><title>Highly Sensitive Multiple microRNA Detection Based on Fluorescence Quenching of Graphene Oxide and Isothermal Strand-Displacement Polymerase Reaction</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A simple, highly sensitive, and selective multiple microRNA (miRNA) detection method based on the graphene oxide (GO) fluorescence quenching and isothermal strand-displacement polymerase reaction (ISDPR) was proposed. The capability to discriminate ssDNA and double-stranded nucleic acid structure coupled with the extraordinary fluorescence quenching of GO on multiple organic dye allows the proposed strategy to simultaneously and selectively detect several miRNA labeled with different dyes in the same solution, while the ISDPR amplification endows the detection method with high sensitivity. The strong interaction between ssDNA and GO led to the fluorescent ssDNA probe exhibiting minimal background fluorescence. Upon the recognition of specific target miRNA, an ISDPR was triggered to produce numerous massive specific DNA-miRNA duplex helixes, and a strong emission was observed due to the weak interaction between the DNA-miRNA duplex helix and GO. A miRNA biosensor down to 2.1 fM with a linear range of 4 orders of magnitude was obtained. Furthermore, the large planar surface of GO allows simultaneous quenching of several DNA probes with different dyes and produces a multiple biosensing platform with high sensitivity and selectivity, which has promising application in profiling the pattern of miRNA expression and biomedical research.</description><subject>Analytical chemistry</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>General, instrumentation</subject><subject>Graphite - chemistry</subject><subject>Luminescence quenching</subject><subject>Methods. Procedures. Technologies</subject><subject>MicroRNAs - analysis</subject><subject>Nucleic Acid Amplification Techniques - instrumentation</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Oxides - chemistry</subject><subject>Polymerase chain reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Fluorescence</subject><subject>Various methods and equipments</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkd1u1DAQhS0EokvhghdAlhASXAT80ziby9LSH6lQaOE6mjjjrivHTm0HsQ_C--KlS4t6NZbn85nxOYS85Ow9Z4J_AC0ZawSfH5EFrwWr1HIpHpMFY0xWomFshzxL6ZoxzhlXT8mOEHV5yJYL8vvEXq3cml6iTzbbn0g_zy7bySEdrY7h4ss-PcSMOtvg6UdIONByOHJziJg0eo3021zKyvorGgw9jjCt0CM9_2UHpOAHeppCXmEcwdHLHMtNdWjT5EDjiD7Tr8GtR4xFml4g_B30nDwx4BK-2NZd8uPo0_eDk-rs_Pj0YP-sgj3Z5gpNrYZ-0DAIbEAqEKYxRjeGIfQ1RzRQWm3fYo1SsYHXhvfFKS6Xqmcg5S55e6s7xXAzY8rdaMunnAOPYU5dsUu2vOVqg75-gF6HOfqy3YaqVXGzVYV6d0sV61KKaLop2hHiukDdJqvuLqvCvtoqzv2Iwx35L5wCvNkCkDQ4U6zTNt1zdVsC3RP3HOj0_1YPB_4Bd2WqGA</recordid><startdate>20120515</startdate><enddate>20120515</enddate><creator>Dong, Haifeng</creator><creator>Zhang, Jing</creator><creator>Ju, Huangxian</creator><creator>Lu, Huiting</creator><creator>Wang, Shiyan</creator><creator>Jin, Shi</creator><creator>Hao, Kaihong</creator><creator>Du, Hongwu</creator><creator>Zhang, Xueji</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120515</creationdate><title>Highly Sensitive Multiple microRNA Detection Based on Fluorescence Quenching of Graphene Oxide and Isothermal Strand-Displacement Polymerase Reaction</title><author>Dong, Haifeng ; Zhang, Jing ; Ju, Huangxian ; Lu, Huiting ; Wang, Shiyan ; Jin, Shi ; Hao, Kaihong ; Du, Hongwu ; Zhang, Xueji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a439t-ef56dbdcad2e7a36a2f7ffc7f0eab51eefaad29b9e5e360d15f1b0071386b0a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical chemistry</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>General, instrumentation</topic><topic>Graphite - chemistry</topic><topic>Luminescence quenching</topic><topic>Methods. Procedures. Technologies</topic><topic>MicroRNAs - analysis</topic><topic>Nucleic Acid Amplification Techniques - instrumentation</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Oxides - chemistry</topic><topic>Polymerase chain reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Fluorescence</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Haifeng</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Ju, Huangxian</creatorcontrib><creatorcontrib>Lu, Huiting</creatorcontrib><creatorcontrib>Wang, Shiyan</creatorcontrib><creatorcontrib>Jin, Shi</creatorcontrib><creatorcontrib>Hao, Kaihong</creatorcontrib><creatorcontrib>Du, Hongwu</creatorcontrib><creatorcontrib>Zhang, Xueji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Haifeng</au><au>Zhang, Jing</au><au>Ju, Huangxian</au><au>Lu, Huiting</au><au>Wang, Shiyan</au><au>Jin, Shi</au><au>Hao, Kaihong</au><au>Du, Hongwu</au><au>Zhang, Xueji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly Sensitive Multiple microRNA Detection Based on Fluorescence Quenching of Graphene Oxide and Isothermal Strand-Displacement Polymerase Reaction</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2012-05-15</date><risdate>2012</risdate><volume>84</volume><issue>10</issue><spage>4587</spage><epage>4593</epage><pages>4587-4593</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A simple, highly sensitive, and selective multiple microRNA (miRNA) detection method based on the graphene oxide (GO) fluorescence quenching and isothermal strand-displacement polymerase reaction (ISDPR) was proposed. The capability to discriminate ssDNA and double-stranded nucleic acid structure coupled with the extraordinary fluorescence quenching of GO on multiple organic dye allows the proposed strategy to simultaneously and selectively detect several miRNA labeled with different dyes in the same solution, while the ISDPR amplification endows the detection method with high sensitivity. The strong interaction between ssDNA and GO led to the fluorescent ssDNA probe exhibiting minimal background fluorescence. Upon the recognition of specific target miRNA, an ISDPR was triggered to produce numerous massive specific DNA-miRNA duplex helixes, and a strong emission was observed due to the weak interaction between the DNA-miRNA duplex helix and GO. A miRNA biosensor down to 2.1 fM with a linear range of 4 orders of magnitude was obtained. Furthermore, the large planar surface of GO allows simultaneous quenching of several DNA probes with different dyes and produces a multiple biosensing platform with high sensitivity and selectivity, which has promising application in profiling the pattern of miRNA expression and biomedical research.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>22510208</pmid><doi>10.1021/ac300721u</doi><tpages>7</tpages></addata></record> |
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| subjects | Analytical chemistry Biochemistry Biological and medical sciences Biosensors Biotechnology Chemistry Exact sciences and technology Fluorescent Dyes - chemistry Fundamental and applied biological sciences. Psychology Gene expression General, instrumentation Graphite - chemistry Luminescence quenching Methods. Procedures. Technologies MicroRNAs - analysis Nucleic Acid Amplification Techniques - instrumentation Nucleic Acid Amplification Techniques - methods Oxides - chemistry Polymerase chain reaction Ribonucleic acid RNA Spectrometric and optical methods Spectrometry, Fluorescence Various methods and equipments |
| title | Highly Sensitive Multiple microRNA Detection Based on Fluorescence Quenching of Graphene Oxide and Isothermal Strand-Displacement Polymerase Reaction |
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