Kinetic and thermodynamic characterization of the functional properties of a hybrid versatile peroxidase using isothermal titration calorimetry: Insight into manganese peroxidase activation and lignin peroxidase inhibition

Isothermal titration calorimetry (ITC) was developed for measuring lignin peroxidase (LiP) and manganese peroxidase (MnP) activities of versatile peroxidase (VP) from Bjerkandera adusta. Developing an ITC approach provided an alternative to colorimetric methods that enabled reaction kinetics to be a...

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Veröffentlicht in:	Biochimie 2012-05, Vol.94 (5), p.1221-1231
Hauptverfasser:	Ertan, Haluk, Siddiqui, Khawar Sohail, Muenchhoff, Julia, Charlton, Tim, Cavicchioli, Ricardo
Format:	Artikel
Sprache:	eng
Schlagworte:	Allosteric substrate activation and inhibition Azo-dye Bjerkandera adusta peroxidase Calorimetry - methods Coriolaceae - enzymology Enzyme kinetics and activation thermodynamics Fungal Proteins - metabolism Kinetics Methylene blue Peroxidases - metabolism Thermodynamics
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