Enzymatic fluorometric microplate assay for quantitative analysis of 3-hydroxybutyric acid in mouse plasma

3-Hydroxybutyric acid (3HB) is a ketone body and acts as an indicator of energy balance and a central regulator of energy homeostasis. We report the application of a sensitive fluorometric assay for the quantitative determination of 3HB. The assay is based on the oxidation of 3HB by 3HB dehydrogenas...

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Veröffentlicht in:	Analytical biochemistry 2012-06, Vol.425 (2), p.114-116
Hauptverfasser:	Takayama, Naoyuki, Ohara, Tatsuya, Yamamoto, Norio
Format:	Artikel
Sprache:	eng
Schlagworte:	3-Hydroxybutyric acid 3-Hydroxybutyric Acid - blood 3-Hydroxybutyric acid dehydrogenase Animals Blood dehydrogenases energy balance Energy Metabolism Fluorometry homeostasis In vivo in vivo studies Ketone bodies ketones Male Mice Mice, Inbred ICR Oxazines - chemistry oxidation Oxidation-Reduction Oxidoreductases - metabolism quantitative analysis Resazurin Resorufin
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