Enzymatic fluorometric microplate assay for quantitative analysis of 3-hydroxybutyric acid in mouse plasma
3-Hydroxybutyric acid (3HB) is a ketone body and acts as an indicator of energy balance and a central regulator of energy homeostasis. We report the application of a sensitive fluorometric assay for the quantitative determination of 3HB. The assay is based on the oxidation of 3HB by 3HB dehydrogenas...
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| Veröffentlicht in: | Analytical biochemistry 2012-06, Vol.425 (2), p.114-116 |
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| container_end_page | 116 |
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| container_title | Analytical biochemistry |
| container_volume | 425 |
| creator | Takayama, Naoyuki Ohara, Tatsuya Yamamoto, Norio |
| description | 3-Hydroxybutyric acid (3HB) is a ketone body and acts as an indicator of energy balance and a central regulator of energy homeostasis. We report the application of a sensitive fluorometric assay for the quantitative determination of 3HB. The assay is based on the oxidation of 3HB by 3HB dehydrogenase and on the diaphorase–resazurin amplifying system. This simple assay enables the measurement of changes in 3HB levels in the blood of normal mice by very small volume sample collection. Therefore, this assay will be useful for in vivo studies of small animals. |
| doi_str_mv | 10.1016/j.ab.2012.03.011 |
| format | Article |
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We report the application of a sensitive fluorometric assay for the quantitative determination of 3HB. The assay is based on the oxidation of 3HB by 3HB dehydrogenase and on the diaphorase–resazurin amplifying system. This simple assay enables the measurement of changes in 3HB levels in the blood of normal mice by very small volume sample collection. 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We report the application of a sensitive fluorometric assay for the quantitative determination of 3HB. The assay is based on the oxidation of 3HB by 3HB dehydrogenase and on the diaphorase–resazurin amplifying system. This simple assay enables the measurement of changes in 3HB levels in the blood of normal mice by very small volume sample collection. Therefore, this assay will be useful for in vivo studies of small animals.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22449496</pmid><doi>10.1016/j.ab.2012.03.011</doi><tpages>3</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 2012-06, Vol.425 (2), p.114-116 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 3-Hydroxybutyric acid 3-Hydroxybutyric Acid - blood 3-Hydroxybutyric acid dehydrogenase Animals Blood dehydrogenases energy balance Energy Metabolism Fluorometry homeostasis In vivo in vivo studies Ketone bodies ketones Male Mice Mice, Inbred ICR Oxazines - chemistry oxidation Oxidation-Reduction Oxidoreductases - metabolism quantitative analysis Resazurin Resorufin |
| title | Enzymatic fluorometric microplate assay for quantitative analysis of 3-hydroxybutyric acid in mouse plasma |
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