Protective Effect of Melatonin on Liver Ischemia-Reperfusion Induced Pulmonary Microvascular Injury in Rats
Abstract Objective Reactive oxygen species generated during liver reperfusion have been implicated in remote lung injury. In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability. Methods Male Sprague-Dawley rats were divi...
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| Veröffentlicht in: | Transplantation proceedings 2012-05, Vol.44 (4), p.962-965 |
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| description | Abstract Objective Reactive oxygen species generated during liver reperfusion have been implicated in remote lung injury. In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability. Methods Male Sprague-Dawley rats were divided into three groups: shame-operated, liver ischemia-reperfusion (I/R), and melatonin pretreated (15 mg/kg, intraperitoneally) 15 minutes prior to the liver I/R). The duration of ischemia was 30 minutes, followed by 2 hours of reperfusion. Lungs were isolated in situ and parameters of the capillary filtration coefficient (Kfc ), lung wet-to-dry weight ratio (W/D), lung weight-to-body weight (LW/BW), and protein concentration in bronchial lavage fluid (PCBAL), the percentage of macrophages and neutrophils in bronchial lavage fluid (BALF), and lung tissue malonedealdehyde were used to assess the lung injury. Results Liver I/R-induced lung injury was noted by the markedly increased Kfc , W/D, LW/BW, PCBAL, and the presence of neutrophils and macrophages in BALF. Lipid peroxidation was also increased ( P < .05). All indicators were markedly decreased in melatonin-pretreated rats ( P < .05), suggesting that lung injury was attenuated. Conclusions Melatonin pretreatment prior to liver I/R can effectively reduce the pulmonary microvascular permeability and attenuate lipid peroxidation in the lungs. |
| doi_str_mv | 10.1016/j.transproceed.2012.01.097 |
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In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability. Methods Male Sprague-Dawley rats were divided into three groups: shame-operated, liver ischemia-reperfusion (I/R), and melatonin pretreated (15 mg/kg, intraperitoneally) 15 minutes prior to the liver I/R). The duration of ischemia was 30 minutes, followed by 2 hours of reperfusion. Lungs were isolated in situ and parameters of the capillary filtration coefficient (Kfc ), lung wet-to-dry weight ratio (W/D), lung weight-to-body weight (LW/BW), and protein concentration in bronchial lavage fluid (PCBAL), the percentage of macrophages and neutrophils in bronchial lavage fluid (BALF), and lung tissue malonedealdehyde were used to assess the lung injury. Results Liver I/R-induced lung injury was noted by the markedly increased Kfc , W/D, LW/BW, PCBAL, and the presence of neutrophils and macrophages in BALF. Lipid peroxidation was also increased ( P < .05). All indicators were markedly decreased in melatonin-pretreated rats ( P < .05), suggesting that lung injury was attenuated. Conclusions Melatonin pretreatment prior to liver I/R can effectively reduce the pulmonary microvascular permeability and attenuate lipid peroxidation in the lungs.</description><identifier>ISSN: 0041-1345</identifier><identifier>EISSN: 1873-2623</identifier><identifier>DOI: 10.1016/j.transproceed.2012.01.097</identifier><identifier>PMID: 22564597</identifier><identifier>CODEN: TRPPA8</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Acute Lung Injury - etiology ; Acute Lung Injury - metabolism ; Acute Lung Injury - pathology ; Acute Lung Injury - prevention & control ; Animals ; Antioxidants - pharmacology ; Biological and medical sciences ; Capillary Permeability - drug effects ; Cytoprotection ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gastroenterology. Liver. Pancreas. Abdomen ; Lipid Peroxidation - drug effects ; Liver - drug effects ; Liver - metabolism ; Liver Diseases - complications ; Liver Diseases - drug therapy ; Liver Diseases - metabolism ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Lung - blood supply ; Lung - drug effects ; Lung - metabolism ; Lung - pathology ; Macrophages - drug effects ; Macrophages - pathology ; Male ; Malondialdehyde - metabolism ; Medical sciences ; Melatonin - pharmacology ; Microvessels - drug effects ; Microvessels - metabolism ; Microvessels - pathology ; Neutrophil Infiltration - drug effects ; Other diseases. Semiology ; Oxidative Stress - drug effects ; Pulmonary Edema - etiology ; Pulmonary Edema - prevention & control ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - complications ; Reperfusion Injury - drug therapy ; Reperfusion Injury - metabolism ; Surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tissue, organ and graft immunology</subject><ispartof>Transplantation proceedings, 2012-05, Vol.44 (4), p.962-965</ispartof><rights>Elsevier Inc.</rights><rights>2012 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c531t-b7d93495509efe26961c0b3664c39e742290e227e520f8b896840f8fa383b93</citedby><cites>FETCH-LOGICAL-c531t-b7d93495509efe26961c0b3664c39e742290e227e520f8b896840f8fa383b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0041134512001327$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25943395$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22564597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiu, M.-H</creatorcontrib><creatorcontrib>Su, C.-L</creatorcontrib><creatorcontrib>Chen, C.-F</creatorcontrib><creatorcontrib>Chen, K.-H</creatorcontrib><creatorcontrib>Wang, D</creatorcontrib><creatorcontrib>Wang, J.-J</creatorcontrib><title>Protective Effect of Melatonin on Liver Ischemia-Reperfusion Induced Pulmonary Microvascular Injury in Rats</title><title>Transplantation proceedings</title><addtitle>Transplant Proc</addtitle><description>Abstract Objective Reactive oxygen species generated during liver reperfusion have been implicated in remote lung injury. In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability. Methods Male Sprague-Dawley rats were divided into three groups: shame-operated, liver ischemia-reperfusion (I/R), and melatonin pretreated (15 mg/kg, intraperitoneally) 15 minutes prior to the liver I/R). The duration of ischemia was 30 minutes, followed by 2 hours of reperfusion. Lungs were isolated in situ and parameters of the capillary filtration coefficient (Kfc ), lung wet-to-dry weight ratio (W/D), lung weight-to-body weight (LW/BW), and protein concentration in bronchial lavage fluid (PCBAL), the percentage of macrophages and neutrophils in bronchial lavage fluid (BALF), and lung tissue malonedealdehyde were used to assess the lung injury. Results Liver I/R-induced lung injury was noted by the markedly increased Kfc , W/D, LW/BW, PCBAL, and the presence of neutrophils and macrophages in BALF. Lipid peroxidation was also increased ( P < .05). All indicators were markedly decreased in melatonin-pretreated rats ( P < .05), suggesting that lung injury was attenuated. Conclusions Melatonin pretreatment prior to liver I/R can effectively reduce the pulmonary microvascular permeability and attenuate lipid peroxidation in the lungs.</description><subject>Acute Lung Injury - etiology</subject><subject>Acute Lung Injury - metabolism</subject><subject>Acute Lung Injury - pathology</subject><subject>Acute Lung Injury - prevention & control</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Capillary Permeability - drug effects</subject><subject>Cytoprotection</subject><subject>Disease Models, Animal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver Diseases - complications</subject><subject>Liver Diseases - drug therapy</subject><subject>Liver Diseases - metabolism</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Lung - blood supply</subject><subject>Lung - drug effects</subject><subject>Lung - metabolism</subject><subject>Lung - pathology</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - pathology</subject><subject>Male</subject><subject>Malondialdehyde - metabolism</subject><subject>Medical sciences</subject><subject>Melatonin - pharmacology</subject><subject>Microvessels - drug effects</subject><subject>Microvessels - metabolism</subject><subject>Microvessels - pathology</subject><subject>Neutrophil Infiltration - drug effects</subject><subject>Other diseases. Semiology</subject><subject>Oxidative Stress - drug effects</subject><subject>Pulmonary Edema - etiology</subject><subject>Pulmonary Edema - prevention & control</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - complications</subject><subject>Reperfusion Injury - drug therapy</subject><subject>Reperfusion Injury - metabolism</subject><subject>Surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Tissue, organ and graft immunology</subject><issn>0041-1345</issn><issn>1873-2623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkktv1DAQgC0EotvCX0AREhKXBL_ixByQUGlhpa2oWu6W40yE06y92MlK_ffMarcCceLkx3wzY382IW8ZrRhl6sNYzcmGvEvRAfQVp4xXlFVUN8_IirWNKLni4jlZUSpZyYSsz8h5ziPFNZfiJTnjvFay1s2KPNymOIOb_R6Kq2HAWRGH4gYmO8fgQxFDscFYKtbZ_YStt-Ud7CANS_YYWod-cdAXt8u0jcGmx-LGuxT3NrtlspgUxgU3sc6dnfMr8mKwU4bXp_GC3F9f_bj8Vm6-f11fft6UrhZsLrum10LquqYaBuBKK-ZoJ5SSTmhoJOeaAucN1JwObddq1UqcDFa0otPigrw_VkU_vxbIs9n67GCabIC4ZIMKWSu5altEPx5RPHPOCQazS36L10DowCkzmr9Vm4NqQ5lB1Zj85tRn6bYYe0p9covAuxOAOuw0YCHn8x-u1lIIXSP35cgBOtl7SCY7DwG9-oTvYfro_-88n_4p4yYfPHZ-gEfIY1xSQOuGmYw55v7wOQ5_g3FKmeCN-A2WKri_</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Chiu, M.-H</creator><creator>Su, C.-L</creator><creator>Chen, C.-F</creator><creator>Chen, K.-H</creator><creator>Wang, D</creator><creator>Wang, J.-J</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Protective Effect of Melatonin on Liver Ischemia-Reperfusion Induced Pulmonary Microvascular Injury in Rats</title><author>Chiu, M.-H ; Su, C.-L ; Chen, C.-F ; Chen, K.-H ; Wang, D ; Wang, J.-J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c531t-b7d93495509efe26961c0b3664c39e742290e227e520f8b896840f8fa383b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acute Lung Injury - etiology</topic><topic>Acute Lung Injury - metabolism</topic><topic>Acute Lung Injury - pathology</topic><topic>Acute Lung Injury - prevention & control</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Capillary Permeability - drug effects</topic><topic>Cytoprotection</topic><topic>Disease Models, Animal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Liver Diseases - complications</topic><topic>Liver Diseases - drug therapy</topic><topic>Liver Diseases - metabolism</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Lung - blood supply</topic><topic>Lung - drug effects</topic><topic>Lung - metabolism</topic><topic>Lung - pathology</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - pathology</topic><topic>Male</topic><topic>Malondialdehyde - metabolism</topic><topic>Medical sciences</topic><topic>Melatonin - pharmacology</topic><topic>Microvessels - drug effects</topic><topic>Microvessels - metabolism</topic><topic>Microvessels - pathology</topic><topic>Neutrophil Infiltration - drug effects</topic><topic>Other diseases. Semiology</topic><topic>Oxidative Stress - drug effects</topic><topic>Pulmonary Edema - etiology</topic><topic>Pulmonary Edema - prevention & control</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - complications</topic><topic>Reperfusion Injury - drug therapy</topic><topic>Reperfusion Injury - metabolism</topic><topic>Surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Tissue, organ and graft immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiu, M.-H</creatorcontrib><creatorcontrib>Su, C.-L</creatorcontrib><creatorcontrib>Chen, C.-F</creatorcontrib><creatorcontrib>Chen, K.-H</creatorcontrib><creatorcontrib>Wang, D</creatorcontrib><creatorcontrib>Wang, J.-J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation proceedings</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiu, M.-H</au><au>Su, C.-L</au><au>Chen, C.-F</au><au>Chen, K.-H</au><au>Wang, D</au><au>Wang, J.-J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective Effect of Melatonin on Liver Ischemia-Reperfusion Induced Pulmonary Microvascular Injury in Rats</atitle><jtitle>Transplantation proceedings</jtitle><addtitle>Transplant Proc</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>44</volume><issue>4</issue><spage>962</spage><epage>965</epage><pages>962-965</pages><issn>0041-1345</issn><eissn>1873-2623</eissn><coden>TRPPA8</coden><abstract>Abstract Objective Reactive oxygen species generated during liver reperfusion have been implicated in remote lung injury. In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability. Methods Male Sprague-Dawley rats were divided into three groups: shame-operated, liver ischemia-reperfusion (I/R), and melatonin pretreated (15 mg/kg, intraperitoneally) 15 minutes prior to the liver I/R). The duration of ischemia was 30 minutes, followed by 2 hours of reperfusion. Lungs were isolated in situ and parameters of the capillary filtration coefficient (Kfc ), lung wet-to-dry weight ratio (W/D), lung weight-to-body weight (LW/BW), and protein concentration in bronchial lavage fluid (PCBAL), the percentage of macrophages and neutrophils in bronchial lavage fluid (BALF), and lung tissue malonedealdehyde were used to assess the lung injury. Results Liver I/R-induced lung injury was noted by the markedly increased Kfc , W/D, LW/BW, PCBAL, and the presence of neutrophils and macrophages in BALF. Lipid peroxidation was also increased ( P < .05). All indicators were markedly decreased in melatonin-pretreated rats ( P < .05), suggesting that lung injury was attenuated. Conclusions Melatonin pretreatment prior to liver I/R can effectively reduce the pulmonary microvascular permeability and attenuate lipid peroxidation in the lungs.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>22564597</pmid><doi>10.1016/j.transproceed.2012.01.097</doi><tpages>4</tpages></addata></record> |
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| subjects | Acute Lung Injury - etiology Acute Lung Injury - metabolism Acute Lung Injury - pathology Acute Lung Injury - prevention & control Animals Antioxidants - pharmacology Biological and medical sciences Capillary Permeability - drug effects Cytoprotection Disease Models, Animal Fundamental and applied biological sciences. Psychology Fundamental immunology Gastroenterology. Liver. Pancreas. Abdomen Lipid Peroxidation - drug effects Liver - drug effects Liver - metabolism Liver Diseases - complications Liver Diseases - drug therapy Liver Diseases - metabolism Liver. Biliary tract. Portal circulation. Exocrine pancreas Lung - blood supply Lung - drug effects Lung - metabolism Lung - pathology Macrophages - drug effects Macrophages - pathology Male Malondialdehyde - metabolism Medical sciences Melatonin - pharmacology Microvessels - drug effects Microvessels - metabolism Microvessels - pathology Neutrophil Infiltration - drug effects Other diseases. Semiology Oxidative Stress - drug effects Pulmonary Edema - etiology Pulmonary Edema - prevention & control Rats Rats, Sprague-Dawley Reperfusion Injury - complications Reperfusion Injury - drug therapy Reperfusion Injury - metabolism Surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tissue, organ and graft immunology |
| title | Protective Effect of Melatonin on Liver Ischemia-Reperfusion Induced Pulmonary Microvascular Injury in Rats |
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