Enhancement of rAAV2-mediated transgene expression in retina cells in vitro and in vivo by coadministration of low-dose chemotherapeutic drugs

Recombinant adeno-associated viral vector serotype 2 (rAAV2) has been used with success to deliver retina-targeted gene therapeutics in retinal degeneration. However, one of the major limitations of this approach is the vector's low transduction efficiency. This study is designed to increase AA...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Investigative ophthalmology & visual science 2012-05, Vol.53 (6), p.2675-2684
Hauptverfasser:	Zhang, Shenghai, Wu, Jihong, Wu, Xiaobing, Xu, Ping, Tian, Yuhua, Yi, Miaoying, Liu, Xinjian, Dong, Xiaoyan, Wolf, Frank, Li, Chuanyuan, Huang, Qian
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antineoplastic Agents - administration & dosage Antineoplastic Agents - pharmacology Blotting, Western Cells, Cultured Ciliary Neurotrophic Factor Dependovirus - genetics Flow Cytometry Gene Expression Regulation - drug effects Genes, Reporter Genetic Vectors Green Fluorescent Proteins - genetics Humans Luciferases - genetics Mice Mice, Inbred BALB C Microscopy, Fluorescence Rats Rats, Mutant Strains Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Retina - metabolism Retinal Pigment Epithelium - metabolism RNA, Messenger - metabolism Transgenes
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!