Isolation and identification of an endophytic fungus associated with Juglans regia L. and its bioactivity

[Objective] This study was to screen out endophytic fungal strains from the bark of Juglans regia L. [Method] The bark of J. regia was washed with running water and placed on medium to let the fungi grown; the fungal colonies were studied via morphological observation and anti-microbial activity. [Result] An endophytic fungus showing broad-scope, highly active anti-microbial activity was screened out. This fungus showed anti-microbial activity seven Gram-positive and four Gram-negative bacterial indicators, six skin pathogenic fungi and seven pathogenic fungi to different extent. Morphological observation on the ITS sequence showed that this fungus is consistent with Paecilomyces lilacinus in genus Paecilomyces bainier(with homology of over 99%). Accordingly this fungus was designated as Paecilomyces lilacinus JY-1024. [Conclusion] This study for the first time reported the isolation of P. lilacinus from J. regia and its in vitro antimicrobial activity to several pathogens.