Complete Monosaccharide Analysis by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important com...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2012-05, Vol.84 (9), p.4104-4110 |
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| creator | Zhang, Zhenqing Khan, Nazeer M Nunez, Karen M Chess, Edward K Szabo, Christina M |
| description | Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high-performance anion-exchange chromatography method with pulsed amperometric detection was initially developed to analyze acidic sugars including different uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method has a limit of quantitation of ∼12.5 × 10–3 nmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety of complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed. |
| doi_str_mv | 10.1021/ac300176z |
| format | Article |
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Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high-performance anion-exchange chromatography method with pulsed amperometric detection was initially developed to analyze acidic sugars including different uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method has a limit of quantitation of ∼12.5 × 10–3 nmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety of complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac300176z</identifier><identifier>PMID: 22448871</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Alginates - chemistry ; Analytical chemistry ; Anions - chemistry ; Carbohydrate Sequence ; Carbohydrates ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography ; Chromatography, Ion Exchange - methods ; Electrochemical methods ; Exact sciences and technology ; Glucuronic Acid - chemistry ; Hexuronic Acids - chemistry ; Ion exchange ; Limit of Detection ; Linear Models ; Molecular Sequence Data ; Monosaccharides - chemistry ; Other chromatographic methods ; Polysaccharides - chemistry ; Sugar</subject><ispartof>Analytical chemistry (Washington), 2012-05, Vol.84 (9), p.4104-4110</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society May 1, 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a373t-fabd9382b3f15c5af90172d3070e115294db73df1480a0944e1535122ea597633</citedby><cites>FETCH-LOGICAL-a373t-fabd9382b3f15c5af90172d3070e115294db73df1480a0944e1535122ea597633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac300176z$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac300176z$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25862717$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22448871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Zhenqing</creatorcontrib><creatorcontrib>Khan, Nazeer M</creatorcontrib><creatorcontrib>Nunez, Karen M</creatorcontrib><creatorcontrib>Chess, Edward K</creatorcontrib><creatorcontrib>Szabo, Christina M</creatorcontrib><title>Complete Monosaccharide Analysis by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high-performance anion-exchange chromatography method with pulsed amperometric detection was initially developed to analyze acidic sugars including different uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method has a limit of quantitation of ∼12.5 × 10–3 nmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety of complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed.</description><subject>Alginates - chemistry</subject><subject>Analytical chemistry</subject><subject>Anions - chemistry</subject><subject>Carbohydrate Sequence</subject><subject>Carbohydrates</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Electrochemical methods</subject><subject>Exact sciences and technology</subject><subject>Glucuronic Acid - chemistry</subject><subject>Hexuronic Acids - chemistry</subject><subject>Ion exchange</subject><subject>Limit of Detection</subject><subject>Linear Models</subject><subject>Molecular Sequence Data</subject><subject>Monosaccharides - chemistry</subject><subject>Other chromatographic methods</subject><subject>Polysaccharides - chemistry</subject><subject>Sugar</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0U1P3DAQBmALgbrLtgf-QGUJIbWHUH_EdnJcLVCQqODQnqOJ42yMkji1E8Hy6-sty4I4-TCPZzyvETqh5JwSRn-A5oRQJZ8P0JwKRhKZZewQzQkhPGGKkBk6DuEhGkqo_IRmjKVplik6R9PKdUNrRoN_ud4F0LoBbyuDlz20m2ADLjf42q6b5N742vkOer0tWtcnl08R92uDV413HYxu7WFoNvjRjg2-n9pgKrzsBhOLZvRW44s4R4_x6md0VEOsf9mdC_Tn6vL36jq5vft5s1reJsAVH5MayirnGSt5TYUWUOdxS1ZxooihcdE8rUrFq5qmGQGSp6mhggvKmAGRK8n5An176Tt493cyYSw6G7RpW-iNm0IR4yCSCylEpKcf6IObfAzhv6Kp5FLQqL6_KO1dCN7UxeBtB34TUbH9i2L_F9F-3XWcys5Ue_kafgRnOwBBQ1v7mK0Nb05kkimq3hzo8P5VHwf-AwxSnF4</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Zhang, Zhenqing</creator><creator>Khan, Nazeer M</creator><creator>Nunez, Karen M</creator><creator>Chess, Edward K</creator><creator>Szabo, Christina M</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Complete Monosaccharide Analysis by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection</title><author>Zhang, Zhenqing ; Khan, Nazeer M ; Nunez, Karen M ; Chess, Edward K ; Szabo, Christina M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a373t-fabd9382b3f15c5af90172d3070e115294db73df1480a0944e1535122ea597633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alginates - chemistry</topic><topic>Analytical chemistry</topic><topic>Anions - chemistry</topic><topic>Carbohydrate Sequence</topic><topic>Carbohydrates</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>Electrochemical methods</topic><topic>Exact sciences and technology</topic><topic>Glucuronic Acid - chemistry</topic><topic>Hexuronic Acids - chemistry</topic><topic>Ion exchange</topic><topic>Limit of Detection</topic><topic>Linear Models</topic><topic>Molecular Sequence Data</topic><topic>Monosaccharides - chemistry</topic><topic>Other chromatographic methods</topic><topic>Polysaccharides - chemistry</topic><topic>Sugar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zhenqing</creatorcontrib><creatorcontrib>Khan, Nazeer M</creatorcontrib><creatorcontrib>Nunez, Karen M</creatorcontrib><creatorcontrib>Chess, Edward K</creatorcontrib><creatorcontrib>Szabo, Christina M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhenqing</au><au>Khan, Nazeer M</au><au>Nunez, Karen M</au><au>Chess, Edward K</au><au>Szabo, Christina M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete Monosaccharide Analysis by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| subjects | Alginates - chemistry Analytical chemistry Anions - chemistry Carbohydrate Sequence Carbohydrates Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, Ion Exchange - methods Electrochemical methods Exact sciences and technology Glucuronic Acid - chemistry Hexuronic Acids - chemistry Ion exchange Limit of Detection Linear Models Molecular Sequence Data Monosaccharides - chemistry Other chromatographic methods Polysaccharides - chemistry Sugar |
| title | Complete Monosaccharide Analysis by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection |
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