Salmonella infects B cells by macropinocytosis and formation of spacious phagosomes but does not induce pyroptosis in favor of its survival
We have previously reported that Salmonella infects B cells and survives within endosomal-lysosomal compartments. However, the mechanisms used by Salmonella to enter B cells remain unknown. In this study, we have shown that Salmonella induces its own entry by the induction of localized ruffling, mac...
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| Veröffentlicht in: | Microbial pathogenesis 2012-06, Vol.52 (6), p.367-374 |
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| creator | Rosales-Reyes, Roberto Pérez-López, Araceli Sánchez-Gómez, Concepción Hernández-Mote, Rosaura Ruth Castro-Eguiluz, Denisse Ortiz-Navarrete, Vianney Alpuche-Aranda, Celia Mercedes |
| description | We have previously reported that Salmonella infects B cells and survives within endosomal-lysosomal compartments. However, the mechanisms used by Salmonella to enter B cells remain unknown. In this study, we have shown that Salmonella induces its own entry by the induction of localized ruffling, macropinocytosis, and spacious phagosome formation. These events were associated with the rearrangement of actin and microtubule networks. The Salmonella pathogenesis island 1 (SPI-1) was necessary to invade B cells. In contrast to macrophages, B cells were highly resistant to cell death induced by Salmonella. These data demonstrate the ability of Salmonella to infect these non-professional phagocytic cells, where the bacterium can find an ideal intracellular niche to support persistence and the possible dissemination of infection.
► Salmonella induce localized ruffling in B cells. ► Salmonella require SPI-1 to infect B cells. ► B cells are resistant to cell death induced by Salmonella. |
| doi_str_mv | 10.1016/j.micpath.2012.03.007 |
| format | Article |
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► Salmonella induce localized ruffling in B cells. ► Salmonella require SPI-1 to infect B cells. ► B cells are resistant to cell death induced by Salmonella.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2012.03.007</identifier><identifier>PMID: 22475626</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>actin ; Actins - metabolism ; Animals ; B cells ; B-Lymphocytes - microbiology ; B-Lymphocytes - physiology ; bacteria ; Bacterial Proteins ; Bacteriology ; Biological and medical sciences ; cell death ; Cells, Cultured ; Female ; Fundamental and applied biological sciences. Psychology ; Host-Pathogen Interactions ; macrophages ; Macrophages - microbiology ; Macrophages - physiology ; Macropinocytosis ; Mice ; Mice, Inbred BALB C ; Microbiology ; microtubules ; Microtubules - metabolism ; Miscellaneous ; pathogenesis ; phagosomes ; Phagosomes - microbiology ; Pinocytosis ; Pyroptosis ; Salmonella ; Salmonella - genetics ; Salmonella - pathogenicity ; SPI-1 ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>Microbial pathogenesis, 2012-06, Vol.52 (6), p.367-374</ispartof><rights>2012 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-3d18d98ddb0853307f6bc9d1a9a7c757068580c1b6287552bc765791e23fff5f3</citedby><cites>FETCH-LOGICAL-c419t-3d18d98ddb0853307f6bc9d1a9a7c757068580c1b6287552bc765791e23fff5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0882401012000733$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25900067$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22475626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosales-Reyes, Roberto</creatorcontrib><creatorcontrib>Pérez-López, Araceli</creatorcontrib><creatorcontrib>Sánchez-Gómez, Concepción</creatorcontrib><creatorcontrib>Hernández-Mote, Rosaura Ruth</creatorcontrib><creatorcontrib>Castro-Eguiluz, Denisse</creatorcontrib><creatorcontrib>Ortiz-Navarrete, Vianney</creatorcontrib><creatorcontrib>Alpuche-Aranda, Celia Mercedes</creatorcontrib><title>Salmonella infects B cells by macropinocytosis and formation of spacious phagosomes but does not induce pyroptosis in favor of its survival</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>We have previously reported that Salmonella infects B cells and survives within endosomal-lysosomal compartments. However, the mechanisms used by Salmonella to enter B cells remain unknown. In this study, we have shown that Salmonella induces its own entry by the induction of localized ruffling, macropinocytosis, and spacious phagosome formation. These events were associated with the rearrangement of actin and microtubule networks. The Salmonella pathogenesis island 1 (SPI-1) was necessary to invade B cells. In contrast to macrophages, B cells were highly resistant to cell death induced by Salmonella. These data demonstrate the ability of Salmonella to infect these non-professional phagocytic cells, where the bacterium can find an ideal intracellular niche to support persistence and the possible dissemination of infection.
► Salmonella induce localized ruffling in B cells. ► Salmonella require SPI-1 to infect B cells. ► B cells are resistant to cell death induced by Salmonella.</description><subject>actin</subject><subject>Actins - metabolism</subject><subject>Animals</subject><subject>B cells</subject><subject>B-Lymphocytes - microbiology</subject><subject>B-Lymphocytes - physiology</subject><subject>bacteria</subject><subject>Bacterial Proteins</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>cell death</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Host-Pathogen Interactions</subject><subject>macrophages</subject><subject>Macrophages - microbiology</subject><subject>Macrophages - physiology</subject><subject>Macropinocytosis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>microtubules</subject><subject>Microtubules - metabolism</subject><subject>Miscellaneous</subject><subject>pathogenesis</subject><subject>phagosomes</subject><subject>Phagosomes - microbiology</subject><subject>Pinocytosis</subject><subject>Pyroptosis</subject><subject>Salmonella</subject><subject>Salmonella - genetics</subject><subject>Salmonella - pathogenicity</subject><subject>SPI-1</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O1DAQhC0EYoeBRwB8QeIyoe3ETnJCsOJPWonDsmfL8c-uR0kc7GSkeQZemo4ywJGTbemrancVIS8ZFAyYfHcshmAmPT8UHBgvoCwA6kdkx6CVB8aheUx20DT8UAGDK_Is5yMAtFXZPiVXnFe1kFzuyK9b3Q9xdH2vaRi9M3OmH6nBd6bdmQ7apDiFMZrzHHPIVI-W-pgGPYc40uhpnrQJccl0etD3McfBoXCZqY14GeOMrnYxjk5nNNo8wki9PsW0ygPOy0s6hZPun5MnXvfZvbice3L3-dOP66-Hm-9fvl1_uDmYirXzobSssW1jbQeNKEuovexMa5ludW1qUYNsRAOGdZI3tRC8M7UUdcscL733wpd78nbznVL8ubg8qyHkdWU9OtxEYb5QtYJzQFRsKMaQc3JeTSkMOp0RWjmpjurSg1p7UFAq7AF1ry4jlm5w9q_qT_AIvLkAOhvd-6RHE_I_TrTYllyNXm-c11Hp-4TM3S1OkoDTBMMA9uT9RjiM7BRcUtkENxpnQ8I6lY3hP5_9DZhutHw</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Rosales-Reyes, Roberto</creator><creator>Pérez-López, Araceli</creator><creator>Sánchez-Gómez, Concepción</creator><creator>Hernández-Mote, Rosaura Ruth</creator><creator>Castro-Eguiluz, Denisse</creator><creator>Ortiz-Navarrete, Vianney</creator><creator>Alpuche-Aranda, Celia Mercedes</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120601</creationdate><title>Salmonella infects B cells by macropinocytosis and formation of spacious phagosomes but does not induce pyroptosis in favor of its survival</title><author>Rosales-Reyes, Roberto ; Pérez-López, Araceli ; Sánchez-Gómez, Concepción ; Hernández-Mote, Rosaura Ruth ; Castro-Eguiluz, Denisse ; Ortiz-Navarrete, Vianney ; Alpuche-Aranda, Celia Mercedes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-3d18d98ddb0853307f6bc9d1a9a7c757068580c1b6287552bc765791e23fff5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>actin</topic><topic>Actins - metabolism</topic><topic>Animals</topic><topic>B cells</topic><topic>B-Lymphocytes - microbiology</topic><topic>B-Lymphocytes - physiology</topic><topic>bacteria</topic><topic>Bacterial Proteins</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>cell death</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Host-Pathogen Interactions</topic><topic>macrophages</topic><topic>Macrophages - microbiology</topic><topic>Macrophages - physiology</topic><topic>Macropinocytosis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>microtubules</topic><topic>Microtubules - metabolism</topic><topic>Miscellaneous</topic><topic>pathogenesis</topic><topic>phagosomes</topic><topic>Phagosomes - microbiology</topic><topic>Pinocytosis</topic><topic>Pyroptosis</topic><topic>Salmonella</topic><topic>Salmonella - genetics</topic><topic>Salmonella - pathogenicity</topic><topic>SPI-1</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosales-Reyes, Roberto</creatorcontrib><creatorcontrib>Pérez-López, Araceli</creatorcontrib><creatorcontrib>Sánchez-Gómez, Concepción</creatorcontrib><creatorcontrib>Hernández-Mote, Rosaura Ruth</creatorcontrib><creatorcontrib>Castro-Eguiluz, Denisse</creatorcontrib><creatorcontrib>Ortiz-Navarrete, Vianney</creatorcontrib><creatorcontrib>Alpuche-Aranda, Celia Mercedes</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosales-Reyes, Roberto</au><au>Pérez-López, Araceli</au><au>Sánchez-Gómez, Concepción</au><au>Hernández-Mote, Rosaura Ruth</au><au>Castro-Eguiluz, Denisse</au><au>Ortiz-Navarrete, Vianney</au><au>Alpuche-Aranda, Celia Mercedes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salmonella infects B cells by macropinocytosis and formation of spacious phagosomes but does not induce pyroptosis in favor of its survival</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>52</volume><issue>6</issue><spage>367</spage><epage>374</epage><pages>367-374</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>We have previously reported that Salmonella infects B cells and survives within endosomal-lysosomal compartments. However, the mechanisms used by Salmonella to enter B cells remain unknown. In this study, we have shown that Salmonella induces its own entry by the induction of localized ruffling, macropinocytosis, and spacious phagosome formation. These events were associated with the rearrangement of actin and microtubule networks. The Salmonella pathogenesis island 1 (SPI-1) was necessary to invade B cells. In contrast to macrophages, B cells were highly resistant to cell death induced by Salmonella. These data demonstrate the ability of Salmonella to infect these non-professional phagocytic cells, where the bacterium can find an ideal intracellular niche to support persistence and the possible dissemination of infection.
► Salmonella induce localized ruffling in B cells. ► Salmonella require SPI-1 to infect B cells. ► B cells are resistant to cell death induced by Salmonella.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>22475626</pmid><doi>10.1016/j.micpath.2012.03.007</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0882-4010 |
| ispartof | Microbial pathogenesis, 2012-06, Vol.52 (6), p.367-374 |
| issn | 0882-4010 1096-1208 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | actin Actins - metabolism Animals B cells B-Lymphocytes - microbiology B-Lymphocytes - physiology bacteria Bacterial Proteins Bacteriology Biological and medical sciences cell death Cells, Cultured Female Fundamental and applied biological sciences. Psychology Host-Pathogen Interactions macrophages Macrophages - microbiology Macrophages - physiology Macropinocytosis Mice Mice, Inbred BALB C Microbiology microtubules Microtubules - metabolism Miscellaneous pathogenesis phagosomes Phagosomes - microbiology Pinocytosis Pyroptosis Salmonella Salmonella - genetics Salmonella - pathogenicity SPI-1 Virulence Factors - genetics Virulence Factors - metabolism |
| title | Salmonella infects B cells by macropinocytosis and formation of spacious phagosomes but does not induce pyroptosis in favor of its survival |
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