Complete sequence analysis of 18S rDNA based on genomic DNA extraction from individual Demodex mites (Acari: Demodicidae)

[Display omitted] ► Improving Demodex mite gDNA extraction and PCR examination technique. ► gDNA of single Demodex mite has been extracted successfully for the first time. ► It could satisfy the demands of Demodex 18S rDNA study at gene level. ► 18S rDNA is unsuitable for molecular identification at...

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Veröffentlicht in:	Experimental parasitology 2012-05, Vol.131 (1), p.45-51
Hauptverfasser:	Zhao, Ya-E., Xu, Ji-Ru, Hu, Li, Wu, Li-Ping, Wang, Zheng-Hang
Format:	Artikel
Sprache:	eng
Schlagworte:	18S rDNA complete sequence analysis Animals Base Sequence Cloning, Molecular Demodex brevis Demodex canis Demodex folliculorum Demodex mite DNA, Ribosomal - chemistry DNA, Ribosomal - isolation & purification DNA-directed DNA polymerase dogs genetic distance Genome - genetics Genomic DNA extraction mites Mites - classification Mites - genetics Molecular Sequence Data parasitism parasitology PCR determination Phylogenetic relationship Phylogeny Polymerase Chain Reaction ribosomal DNA RNA, Ribosomal, 18S - genetics Sequence Alignment sequence analysis Sequence Analysis, DNA
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