Selection and Identification of a DNA Aptamer Targeted to Vibrio parahemolyticus

A whole-bacterium systemic evolution of ligands by exponential enrichment (SELEX) method was applied to a combinatorial library of FAM-labeled single-stranded DNA molecules to identify DNA aptamers demonstrating specific binding to Vibrio parahemolyticus. FAM-labeled aptamer sequences with high bind...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2012-04, Vol.60 (16), p.4034-4038
Hauptverfasser:	Duan, Nuo, Wu, Shijia, Chen, Xiujuan, Huang, Yukun, Wang, Zhouping
Format:	Artikel
Sprache:	eng
Schlagworte:	Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - genetics binding capacity Biological and medical sciences dissociation DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics flow cytometry Food industries Fundamental and applied biological sciences. Psychology Gene Library Kinetics Nucleic Acid Conformation oligonucleotides SELEX Aptamer Technique - methods single-stranded DNA Vibrio Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - isolation & purification
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