High-voltage power supplies to capillary and microchip electrophoresis
Over the past years, the development of capillary electrophoresis (CE) and microchip electrophoresis (ME) systems has grown due to instrumental simplicity and wide application. In both CE and ME, the application of a high voltage (HV) is a crucial step in the electrokinetic (EK) injection and separa...
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| Veröffentlicht in: | Electrophoresis 2012-03, Vol.33 (6), p.893-898 |
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| container_title | Electrophoresis |
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| creator | Blanes, Lucas Coltro, Wendell Karlos Tomazelli Saito, Renata Mayumi Van Gramberg, Amanda Lucio do Lago, Claudimir Doble, Philip |
| description | Over the past years, the development of capillary electrophoresis (CE) and microchip electrophoresis (ME) systems has grown due to instrumental simplicity and wide application. In both CE and ME, the application of a high voltage (HV) is a crucial step in the electrokinetic (EK) injection and separation processes. Particularly on ME devices, EK injection is often performed with three different modes: gated, pinched, and unpinched. In all these cases, different potential values may be applied to one or multiple channels to control the injection of small sample volumes as well as the separation process. For this reason, the construction of reliable HV power supplies (HVPS) is required. This review covers the advances of the development of commercial and laboratory‐built HVPS for CE and ME. Moreover, it intends to be a guide for new developers of electrophoresis instrumentation. |
| doi_str_mv | 10.1002/elps.201100490 |
| format | Article |
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In both CE and ME, the application of a high voltage (HV) is a crucial step in the electrokinetic (EK) injection and separation processes. Particularly on ME devices, EK injection is often performed with three different modes: gated, pinched, and unpinched. In all these cases, different potential values may be applied to one or multiple channels to control the injection of small sample volumes as well as the separation process. For this reason, the construction of reliable HV power supplies (HVPS) is required. This review covers the advances of the development of commercial and laboratory‐built HVPS for CE and ME. 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Moreover, it intends to be a guide for new developers of electrophoresis instrumentation.</description><subject>Analytical instrumentation</subject><subject>Electric Power Supplies</subject><subject>Electrophoresis, Capillary - instrumentation</subject><subject>Electrophoresis, Microchip - instrumentation</subject><subject>High-voltage power supplies</subject><subject>Lab-on-a-chip</subject><subject>Miniaturization</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1PGzEQxa2Kqgm01x6rPXLZ1Pb4a48IkaQiKhXQcrSc3dnE7YY19gbKf4-jQK6cZkb6vac3j5CvjE4Ypfw7diFNOGX5EBX9QMZMcl5yZeCIjCnTUFIDckSOU_pLd4wQn8iIc8mNoGZMpnO_WpePfTe4FRahf8JYpG0IncdUDH1Ru-C7zsXnwt03xcbXsa_XPhTYYT3EPqz7iMmnz-Rj67qEX17nCfk9vbg9n5eLq9mP87NFWYM2UFYtuCVnqtFcCrc0WrQUwbWCG2WEYlXDKEdJWQUGTd5B1wqYdrpRYHgNJ-R07xti_7DFNNiNTzXmhPfYb5PNNVQSqAKd0ckezZFTitjaEP0mf5KhHcftrjt76C4Lvr16b5cbbA74W1kZEHvgyXf4_I6dvVj8uhFMQpaVe5lPA_4_yFz8Z5UGLe3dz5m9vpS3d9XlH6vgBWzPiIE</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Blanes, Lucas</creator><creator>Coltro, Wendell Karlos Tomazelli</creator><creator>Saito, Renata Mayumi</creator><creator>Van Gramberg, Amanda</creator><creator>Lucio do Lago, Claudimir</creator><creator>Doble, Philip</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201203</creationdate><title>High-voltage power supplies to capillary and microchip electrophoresis</title><author>Blanes, Lucas ; Coltro, Wendell Karlos Tomazelli ; Saito, Renata Mayumi ; Van Gramberg, Amanda ; Lucio do Lago, Claudimir ; Doble, Philip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3783-9f3ab216d7254ab874f0e3af428684619d102e501938e810237c6317a7d6382c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical instrumentation</topic><topic>Electric Power Supplies</topic><topic>Electrophoresis, Capillary - instrumentation</topic><topic>Electrophoresis, Microchip - instrumentation</topic><topic>High-voltage power supplies</topic><topic>Lab-on-a-chip</topic><topic>Miniaturization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blanes, Lucas</creatorcontrib><creatorcontrib>Coltro, Wendell Karlos Tomazelli</creatorcontrib><creatorcontrib>Saito, Renata Mayumi</creatorcontrib><creatorcontrib>Van Gramberg, Amanda</creatorcontrib><creatorcontrib>Lucio do Lago, Claudimir</creatorcontrib><creatorcontrib>Doble, Philip</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blanes, Lucas</au><au>Coltro, Wendell Karlos Tomazelli</au><au>Saito, Renata Mayumi</au><au>Van Gramberg, Amanda</au><au>Lucio do Lago, Claudimir</au><au>Doble, Philip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-voltage power supplies to capillary and microchip electrophoresis</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2012-03</date><risdate>2012</risdate><volume>33</volume><issue>6</issue><spage>893</spage><epage>898</epage><pages>893-898</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Over the past years, the development of capillary electrophoresis (CE) and microchip electrophoresis (ME) systems has grown due to instrumental simplicity and wide application. 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| ispartof | Electrophoresis, 2012-03, Vol.33 (6), p.893-898 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Analytical instrumentation Electric Power Supplies Electrophoresis, Capillary - instrumentation Electrophoresis, Microchip - instrumentation High-voltage power supplies Lab-on-a-chip Miniaturization |
| title | High-voltage power supplies to capillary and microchip electrophoresis |
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