The activation, by antigen, of naïve TCR transgenic CD4 T cells cultured at physiological, rather than artificially high, frequencies more accurately reflects the in vivo activation of normal numbers of naïve CD4+ T cells

► We culture 300–3×106 Rag2−/− DO11.10 CD4+ T cells with APC and peptide antigen. ► The overall level of T cell activation decreases with increasing T cell frequency. ► This decrease is not due to any observed differences at the APC level. ► This decrease is likely due to increased intraclonal compe...

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Veröffentlicht in:	Cellular immunology 2012, Vol.274 (1-2), p.115-120
Hauptverfasser:	Rudulier, Christopher D., Kroeger, David R., Bretscher, Peter A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigen-Presenting Cells - immunology B7-1 Antigen - biosynthesis B7-2 Antigen - biosynthesis CD4-Positive T-Lymphocytes - immunology Cell Culture Techniques Cells, Cultured Cytokines - biosynthesis Hyaluronan Receptors - biosynthesis In vitro L-Selectin - biosynthesis Lymphocyte Activation - immunology Mice Mice, Inbred BALB C Mice, Transgenic Receptors, Antigen, T-Cell - genetics Receptors, Antigen, T-Cell - immunology T cell activation TCR transgenic T cells
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