Evaluation and implementation of a potential diagnostic molecular marker for H1‐conferred potato cyst nematode resistance in potato (Solanum tuberosum L.)
With 1 figure and 1 table ABSTRACT: Potato cyst nematode (PCN) is a major pest of potato crops. Marker‐assisted selection of PCN‐resistant cultivars is consequently a key objective of many potato breeding programmes. The genetic marker TG689, linked to the resistance gene H1, is the current marker o...
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| Veröffentlicht in: | Plant breeding 2012-04, Vol.131 (2), p.315-321 |
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| creator | Schultz, Lee Cogan, Noel O. I McLean, Karen Dale, M. Finlay B Bryan, Glenn J Forster, John W Slater, Anthony T |
| description | With 1 figure and 1 table ABSTRACT: Potato cyst nematode (PCN) is a major pest of potato crops. Marker‐assisted selection of PCN‐resistant cultivars is consequently a key objective of many potato breeding programmes. The genetic marker TG689, linked to the resistance gene H1, is the current marker of choice for the prediction of PCN resistance. Polymorphism at TG689 was evaluated across 373 cultivars from Australian and Scottish breeding programmes, revealing the loss of association between the predictive allele and trait locus. Two recently identified genetic markers flanking the H1 locus were then evaluated for association with PCN‐resistant phenotypes across 73 cultivars. With a single exception, presence or absence of a 452‐bp amplicon for the 57R marker was predictive for the target phenotype. Further evaluation of the 57R marker on more than 300 cultivars from the Australian and Scottish potato breeding programmes provided additional evidence for robust diagnostic capability. To enable routine implementation, the 57R marker was converted into a high‐throughput, fluorochrome detection–based assay and screened across a broader collection of parental cultivars and advanced breeding selections for the prediction of PCN resistance. |
| doi_str_mv | 10.1111/j.1439-0523.2012.01949.x |
| format | Article |
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I ; McLean, Karen ; Dale, M. Finlay B ; Bryan, Glenn J ; Forster, John W ; Slater, Anthony T</creator><creatorcontrib>Schultz, Lee ; Cogan, Noel O. I ; McLean, Karen ; Dale, M. Finlay B ; Bryan, Glenn J ; Forster, John W ; Slater, Anthony T</creatorcontrib><description>With 1 figure and 1 table ABSTRACT: Potato cyst nematode (PCN) is a major pest of potato crops. Marker‐assisted selection of PCN‐resistant cultivars is consequently a key objective of many potato breeding programmes. The genetic marker TG689, linked to the resistance gene H1, is the current marker of choice for the prediction of PCN resistance. Polymorphism at TG689 was evaluated across 373 cultivars from Australian and Scottish breeding programmes, revealing the loss of association between the predictive allele and trait locus. Two recently identified genetic markers flanking the H1 locus were then evaluated for association with PCN‐resistant phenotypes across 73 cultivars. With a single exception, presence or absence of a 452‐bp amplicon for the 57R marker was predictive for the target phenotype. Further evaluation of the 57R marker on more than 300 cultivars from the Australian and Scottish potato breeding programmes provided additional evidence for robust diagnostic capability. To enable routine implementation, the 57R marker was converted into a high‐throughput, fluorochrome detection–based assay and screened across a broader collection of parental cultivars and advanced breeding selections for the prediction of PCN resistance.</description><identifier>ISSN: 0179-9541</identifier><identifier>EISSN: 1439-0523</identifier><identifier>DOI: 10.1111/j.1439-0523.2012.01949.x</identifier><identifier>CODEN: PLABED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; breeding ; Crops ; cultivars ; cyst nematodes ; Cysts ; diagnostic marker ; fluorochromes ; Fundamental and applied biological sciences. Psychology ; genes ; Genetic markers ; Genetics ; Genetics and breeding of economic plants ; Globodera rostochiensis ; H1 gene ; loci ; marker-assisted selection ; Nematoda ; Pests ; phenotype ; Plant breeding ; Potatoes ; prediction ; SCAR-57R ; Solanum tuberosum ; Varietal selection. 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I</creatorcontrib><creatorcontrib>McLean, Karen</creatorcontrib><creatorcontrib>Dale, M. Finlay B</creatorcontrib><creatorcontrib>Bryan, Glenn J</creatorcontrib><creatorcontrib>Forster, John W</creatorcontrib><creatorcontrib>Slater, Anthony T</creatorcontrib><title>Evaluation and implementation of a potential diagnostic molecular marker for H1‐conferred potato cyst nematode resistance in potato (Solanum tuberosum L.)</title><title>Plant breeding</title><description>With 1 figure and 1 table ABSTRACT: Potato cyst nematode (PCN) is a major pest of potato crops. Marker‐assisted selection of PCN‐resistant cultivars is consequently a key objective of many potato breeding programmes. The genetic marker TG689, linked to the resistance gene H1, is the current marker of choice for the prediction of PCN resistance. Polymorphism at TG689 was evaluated across 373 cultivars from Australian and Scottish breeding programmes, revealing the loss of association between the predictive allele and trait locus. Two recently identified genetic markers flanking the H1 locus were then evaluated for association with PCN‐resistant phenotypes across 73 cultivars. With a single exception, presence or absence of a 452‐bp amplicon for the 57R marker was predictive for the target phenotype. Further evaluation of the 57R marker on more than 300 cultivars from the Australian and Scottish potato breeding programmes provided additional evidence for robust diagnostic capability. To enable routine implementation, the 57R marker was converted into a high‐throughput, fluorochrome detection–based assay and screened across a broader collection of parental cultivars and advanced breeding selections for the prediction of PCN resistance.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>breeding</subject><subject>Crops</subject><subject>cultivars</subject><subject>cyst nematodes</subject><subject>Cysts</subject><subject>diagnostic marker</subject><subject>fluorochromes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genetic markers</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>Globodera rostochiensis</subject><subject>H1 gene</subject><subject>loci</subject><subject>marker-assisted selection</subject><subject>Nematoda</subject><subject>Pests</subject><subject>phenotype</subject><subject>Plant breeding</subject><subject>Potatoes</subject><subject>prediction</subject><subject>SCAR-57R</subject><subject>Solanum tuberosum</subject><subject>Varietal selection. 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Polymorphism at TG689 was evaluated across 373 cultivars from Australian and Scottish breeding programmes, revealing the loss of association between the predictive allele and trait locus. Two recently identified genetic markers flanking the H1 locus were then evaluated for association with PCN‐resistant phenotypes across 73 cultivars. With a single exception, presence or absence of a 452‐bp amplicon for the 57R marker was predictive for the target phenotype. Further evaluation of the 57R marker on more than 300 cultivars from the Australian and Scottish potato breeding programmes provided additional evidence for robust diagnostic capability. 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| subjects | Agronomy. Soil science and plant productions Biological and medical sciences breeding Crops cultivars cyst nematodes Cysts diagnostic marker fluorochromes Fundamental and applied biological sciences. Psychology genes Genetic markers Genetics Genetics and breeding of economic plants Globodera rostochiensis H1 gene loci marker-assisted selection Nematoda Pests phenotype Plant breeding Potatoes prediction SCAR-57R Solanum tuberosum Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Evaluation and implementation of a potential diagnostic molecular marker for H1‐conferred potato cyst nematode resistance in potato (Solanum tuberosum L.) |
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