Lipidomic Analysis of Porcine Olfactory Epithelial Membranes and Cilia
The use of the matrix 9-aminoacridine has been recently introduced in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of both anionic and cationic phospholipids. In the present study, we take advantage of this technique to analyze the lipids of porci...
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| Veröffentlicht in: | Lipids 2010-07, Vol.45 (7), p.593-602 |
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| creator | Lobasso, Simona Lopalco, Patrizia Angelini, Roberto Baronio, Maristella Fanizzi, Francesco P Babudri, Francesco Corcelli, Angela |
| description | The use of the matrix 9-aminoacridine has been recently introduced in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of both anionic and cationic phospholipids. In the present study, we take advantage of this technique to analyze the lipids of porcine olfactory mucosa and a membrane fraction enriched in cilia. Thin-layer chromatography (TLC) and ³¹P-NMR analyses of the lipid extracts were also performed in parallel. MALDI-TOF-MS allowed the identification of lipid classes in the total lipid extract and individual lipids present in the main TLC bands. The comparison between the composition of the two lipid extracts showed that: (1) cardiolipin, present in small amount in the whole olfactory mucosa lipid extract, was absent in the extract of membranes enriched in olfactory cilia, (2) phosphatidylethanolamine species were less abundant in ciliary than in whole epithelial membranes, (3) sulfoglycosphingolipids were detected in the lipid extract of ciliary membranes, but not in that of epithelial membranes. Our results indicate that the lipid pattern of ciliary membranes is different from that of whole-tissue membranes and suggest that olfactory receptors require a specific lipid environment for their functioning. |
| doi_str_mv | 10.1007/s11745-010-3432-1 |
| format | Article |
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In the present study, we take advantage of this technique to analyze the lipids of porcine olfactory mucosa and a membrane fraction enriched in cilia. Thin-layer chromatography (TLC) and ³¹P-NMR analyses of the lipid extracts were also performed in parallel. MALDI-TOF-MS allowed the identification of lipid classes in the total lipid extract and individual lipids present in the main TLC bands. The comparison between the composition of the two lipid extracts showed that: (1) cardiolipin, present in small amount in the whole olfactory mucosa lipid extract, was absent in the extract of membranes enriched in olfactory cilia, (2) phosphatidylethanolamine species were less abundant in ciliary than in whole epithelial membranes, (3) sulfoglycosphingolipids were detected in the lipid extract of ciliary membranes, but not in that of epithelial membranes. Our results indicate that the lipid pattern of ciliary membranes is different from that of whole-tissue membranes and suggest that olfactory receptors require a specific lipid environment for their functioning.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/s11745-010-3432-1</identifier><identifier>PMID: 20512424</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>31P‐NMR ; Aminacrine - metabolism ; Animals ; Biomedical and Life Sciences ; cardiolipin ; Cilia - chemistry ; Cilia - metabolism ; Ciliary membranes ; Epithelial Cells - chemistry ; Epithelial Cells - metabolism ; Ionization ; Lasers ; Life Sciences ; Lipidology ; Lipids ; Lipids - analysis ; Lipids - chemistry ; MALDI‐TOF‐MS ; Mass spectrometry ; Mass spectroscopy ; Medical Biochemistry ; Medicinal Chemistry ; Membranes ; Microbial Genetics and Genomics ; Neurochemistry ; Nutrition ; Odorant receptors ; olfactory cilia ; Olfactory epithelium ; Olfactory Mucosa - chemistry ; Olfactory Mucosa - metabolism ; Original Article ; phosphatidylethanolamine ; Phospholipids ; Pig ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Swine ; Thin layer chromatography ; TLC</subject><ispartof>Lipids, 2010-07, Vol.45 (7), p.593-602</ispartof><rights>AOCS 2010</rights><rights>2010 American Oil Chemists' Society (AOCS)</rights><rights>Copyright Springer Science & Business Media Jul 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4753-262918fa912ca391d0a63c22029de32dfc8f2aa571ca857caa5b5a22a7f2c5873</citedby><cites>FETCH-LOGICAL-c4753-262918fa912ca391d0a63c22029de32dfc8f2aa571ca857caa5b5a22a7f2c5873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11745-010-3432-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11745-010-3432-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,41490,42559,45576,45577,51321</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20512424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lobasso, Simona</creatorcontrib><creatorcontrib>Lopalco, Patrizia</creatorcontrib><creatorcontrib>Angelini, Roberto</creatorcontrib><creatorcontrib>Baronio, Maristella</creatorcontrib><creatorcontrib>Fanizzi, Francesco P</creatorcontrib><creatorcontrib>Babudri, Francesco</creatorcontrib><creatorcontrib>Corcelli, Angela</creatorcontrib><title>Lipidomic Analysis of Porcine Olfactory Epithelial Membranes and Cilia</title><title>Lipids</title><addtitle>Lipids</addtitle><addtitle>Lipids</addtitle><description>The use of the matrix 9-aminoacridine has been recently introduced in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of both anionic and cationic phospholipids. 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Our results indicate that the lipid pattern of ciliary membranes is different from that of whole-tissue membranes and suggest that olfactory receptors require a specific lipid environment for their functioning.</description><subject>31P‐NMR</subject><subject>Aminacrine - metabolism</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>cardiolipin</subject><subject>Cilia - chemistry</subject><subject>Cilia - metabolism</subject><subject>Ciliary membranes</subject><subject>Epithelial Cells - chemistry</subject><subject>Epithelial Cells - metabolism</subject><subject>Ionization</subject><subject>Lasers</subject><subject>Life Sciences</subject><subject>Lipidology</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Lipids - chemistry</subject><subject>MALDI‐TOF‐MS</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical Biochemistry</subject><subject>Medicinal Chemistry</subject><subject>Membranes</subject><subject>Microbial Genetics and Genomics</subject><subject>Neurochemistry</subject><subject>Nutrition</subject><subject>Odorant receptors</subject><subject>olfactory cilia</subject><subject>Olfactory epithelium</subject><subject>Olfactory Mucosa - chemistry</subject><subject>Olfactory Mucosa - metabolism</subject><subject>Original Article</subject><subject>phosphatidylethanolamine</subject><subject>Phospholipids</subject><subject>Pig</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Swine</subject><subject>Thin layer 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chemistry</topic><topic>MALDI‐TOF‐MS</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical Biochemistry</topic><topic>Medicinal Chemistry</topic><topic>Membranes</topic><topic>Microbial Genetics and Genomics</topic><topic>Neurochemistry</topic><topic>Nutrition</topic><topic>Odorant receptors</topic><topic>olfactory cilia</topic><topic>Olfactory epithelium</topic><topic>Olfactory Mucosa - chemistry</topic><topic>Olfactory Mucosa - metabolism</topic><topic>Original Article</topic><topic>phosphatidylethanolamine</topic><topic>Phospholipids</topic><topic>Pig</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Swine</topic><topic>Thin layer chromatography</topic><topic>TLC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lobasso, Simona</creatorcontrib><creatorcontrib>Lopalco, Patrizia</creatorcontrib><creatorcontrib>Angelini, Roberto</creatorcontrib><creatorcontrib>Baronio, 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introduced in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of both anionic and cationic phospholipids. In the present study, we take advantage of this technique to analyze the lipids of porcine olfactory mucosa and a membrane fraction enriched in cilia. Thin-layer chromatography (TLC) and ³¹P-NMR analyses of the lipid extracts were also performed in parallel. MALDI-TOF-MS allowed the identification of lipid classes in the total lipid extract and individual lipids present in the main TLC bands. The comparison between the composition of the two lipid extracts showed that: (1) cardiolipin, present in small amount in the whole olfactory mucosa lipid extract, was absent in the extract of membranes enriched in olfactory cilia, (2) phosphatidylethanolamine species were less abundant in ciliary than in whole epithelial membranes, (3) sulfoglycosphingolipids were detected in the lipid extract of ciliary membranes, but not in that of epithelial membranes. Our results indicate that the lipid pattern of ciliary membranes is different from that of whole-tissue membranes and suggest that olfactory receptors require a specific lipid environment for their functioning.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20512424</pmid><doi>10.1007/s11745-010-3432-1</doi><tpages>10</tpages></addata></record> |
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| subjects | 31P‐NMR Aminacrine - metabolism Animals Biomedical and Life Sciences cardiolipin Cilia - chemistry Cilia - metabolism Ciliary membranes Epithelial Cells - chemistry Epithelial Cells - metabolism Ionization Lasers Life Sciences Lipidology Lipids Lipids - analysis Lipids - chemistry MALDI‐TOF‐MS Mass spectrometry Mass spectroscopy Medical Biochemistry Medicinal Chemistry Membranes Microbial Genetics and Genomics Neurochemistry Nutrition Odorant receptors olfactory cilia Olfactory epithelium Olfactory Mucosa - chemistry Olfactory Mucosa - metabolism Original Article phosphatidylethanolamine Phospholipids Pig Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Swine Thin layer chromatography TLC |
| title | Lipidomic Analysis of Porcine Olfactory Epithelial Membranes and Cilia |
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