Toxicogenomic comparison of multi-wall carbon nanotubes (MWCNTs) and asbestos
Carbon nanotubes (CNTs) have specific properties, including electrical and thermal conductivity, great strength, and rigidity, that allow them to be used in many fields. However, this increasing contact with humans and the environment is also raising health and safety concerns. Thus, research on the...
Gespeichert in:
| Veröffentlicht in: | Archives of toxicology 2012-04, Vol.86 (4), p.553-562 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 562 |
|---|---|
| container_issue | 4 |
| container_start_page | 553 |
| container_title | Archives of toxicology |
| container_volume | 86 |
| creator | Kim, Jin Sik Song, Kyung Seuk Lee, Jin Kyu Choi, Young C. Bang, In Seok Kang, Chang Soo Yu, Il Je |
| description | Carbon nanotubes (CNTs) have specific properties, including electrical and thermal conductivity, great strength, and rigidity, that allow them to be used in many fields. However, this increasing contact with humans and the environment is also raising health and safety concerns. Thus, research on the safety of CNTs has attracted much interest, including a comparison of the toxic effects of asbestos and carbon nanotubes, due to their physical similarity of a high aspect ratio (length/diameter). Nonetheless, there has not yet been a toxicogenomic comparison. Therefore, to examine toxicogenomic effects, the 50% growth inhibition (GI
50
) concentration was determined for multi-wall carbon nanotubes (MWCNTs) and asbestos (crocidolite) and found to be approximately 0.0135 and 0.066%, respectively, in the case of 24-h treatment of normal human bronchial epithelia (NHBE) cells. Using these GI
50
concentrations, NHBE cells were then treated with MWCNTs and asbestos for 6 and 24 h, followed by a DNA microarray analysis. Among 31,647 genes, 1,201 and 1,252 were up-regulated by both asbestos and MWCNTs after 6 and 24 h of exposure, respectively. Meanwhile, 1,977 and 1,542 genes were down-regulated by both asbestos and MWNCTs after 6 and 24 h of exposure, respectively. In particular, the asbestos and MWCNTs both induced an over twofold up- and down-regulated expression of 12 mesothelioma-related genes and 22 lung cancer-related genes when compared with the negative control. Plus, the genes induced by the MWCNT exposure were expressed in the brain, lungs, epithelium, liver, and colon. |
| doi_str_mv | 10.1007/s00204-011-0770-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1008834045</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2613421031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-96f85b2ef0aa1ab53530952c898ef87597b18990d41f41218087a90fc0b80e253</originalsourceid><addsrcrecordid>eNp1kMtOwzAQRS0EoqXwAWxQxKosDONHYnuJKl4ShU0Ry8hxnSpVEhc7EfD3uEoBCYmV5Ttn7sxchE4JXBIAcRUAKHAMhGAQAnC2h8aEMxp_TO6jMTAOOBUZGaGjENYAhErFDtGIUogqpGM0X7iPyriVbV1TmcS4ZqN9FVybuDJp-rqr8Luu68RoX0Sx1a3r-sKGZDp_nT0twkWi22WiQ5Q6F47RQanrYE927wS93N4sZvf48fnuYXb9iA3PVIdVVsq0oLYErYkuUpYyUCk1UklbSpEqURCpFCw5KTmhRIIUWkFpoJBgacomaDr4brx76-PovKmCsXWtW-v6kMd0pIzH8y16_gddu963cbtcUQWEc8IiRAbIeBeCt2W-8VWj_Wd02pqJfIg6j1Hn26jzLPac7Yz7orHLn47vbCNAByDEUruy_nfy_65f1EqGyA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>929014413</pqid></control><display><type>article</type><title>Toxicogenomic comparison of multi-wall carbon nanotubes (MWCNTs) and asbestos</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Kim, Jin Sik ; Song, Kyung Seuk ; Lee, Jin Kyu ; Choi, Young C. ; Bang, In Seok ; Kang, Chang Soo ; Yu, Il Je</creator><creatorcontrib>Kim, Jin Sik ; Song, Kyung Seuk ; Lee, Jin Kyu ; Choi, Young C. ; Bang, In Seok ; Kang, Chang Soo ; Yu, Il Je</creatorcontrib><description>Carbon nanotubes (CNTs) have specific properties, including electrical and thermal conductivity, great strength, and rigidity, that allow them to be used in many fields. However, this increasing contact with humans and the environment is also raising health and safety concerns. Thus, research on the safety of CNTs has attracted much interest, including a comparison of the toxic effects of asbestos and carbon nanotubes, due to their physical similarity of a high aspect ratio (length/diameter). Nonetheless, there has not yet been a toxicogenomic comparison. Therefore, to examine toxicogenomic effects, the 50% growth inhibition (GI
50
) concentration was determined for multi-wall carbon nanotubes (MWCNTs) and asbestos (crocidolite) and found to be approximately 0.0135 and 0.066%, respectively, in the case of 24-h treatment of normal human bronchial epithelia (NHBE) cells. Using these GI
50
concentrations, NHBE cells were then treated with MWCNTs and asbestos for 6 and 24 h, followed by a DNA microarray analysis. Among 31,647 genes, 1,201 and 1,252 were up-regulated by both asbestos and MWCNTs after 6 and 24 h of exposure, respectively. Meanwhile, 1,977 and 1,542 genes were down-regulated by both asbestos and MWNCTs after 6 and 24 h of exposure, respectively. In particular, the asbestos and MWCNTs both induced an over twofold up- and down-regulated expression of 12 mesothelioma-related genes and 22 lung cancer-related genes when compared with the negative control. Plus, the genes induced by the MWCNT exposure were expressed in the brain, lungs, epithelium, liver, and colon.</description><identifier>ISSN: 0340-5761</identifier><identifier>EISSN: 1432-0738</identifier><identifier>DOI: 10.1007/s00204-011-0770-6</identifier><identifier>PMID: 22076105</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Asbestos ; Asbestos, Crocidolite - toxicity ; Biomedical and Life Sciences ; Biomedicine ; Bronchi - drug effects ; Carbon ; Cell Line ; Cell Survival - drug effects ; Comparative studies ; Environmental Health ; Gene Expression Profiling ; Gene Expression Regulation - drug effects ; Genomics ; Humans ; Inorganic Compounds ; Nanotubes ; Nanotubes, Carbon - toxicity ; Occupational Medicine/Industrial Medicine ; Oligonucleotide Array Sequence Analysis ; Pharmacology/Toxicology ; Respiratory Mucosa - drug effects ; Toxicogenetics ; Toxicology</subject><ispartof>Archives of toxicology, 2012-04, Vol.86 (4), p.553-562</ispartof><rights>Springer-Verlag 2011</rights><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-96f85b2ef0aa1ab53530952c898ef87597b18990d41f41218087a90fc0b80e253</citedby><cites>FETCH-LOGICAL-c469t-96f85b2ef0aa1ab53530952c898ef87597b18990d41f41218087a90fc0b80e253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00204-011-0770-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00204-011-0770-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22076105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jin Sik</creatorcontrib><creatorcontrib>Song, Kyung Seuk</creatorcontrib><creatorcontrib>Lee, Jin Kyu</creatorcontrib><creatorcontrib>Choi, Young C.</creatorcontrib><creatorcontrib>Bang, In Seok</creatorcontrib><creatorcontrib>Kang, Chang Soo</creatorcontrib><creatorcontrib>Yu, Il Je</creatorcontrib><title>Toxicogenomic comparison of multi-wall carbon nanotubes (MWCNTs) and asbestos</title><title>Archives of toxicology</title><addtitle>Arch Toxicol</addtitle><addtitle>Arch Toxicol</addtitle><description>Carbon nanotubes (CNTs) have specific properties, including electrical and thermal conductivity, great strength, and rigidity, that allow them to be used in many fields. However, this increasing contact with humans and the environment is also raising health and safety concerns. Thus, research on the safety of CNTs has attracted much interest, including a comparison of the toxic effects of asbestos and carbon nanotubes, due to their physical similarity of a high aspect ratio (length/diameter). Nonetheless, there has not yet been a toxicogenomic comparison. Therefore, to examine toxicogenomic effects, the 50% growth inhibition (GI
50
) concentration was determined for multi-wall carbon nanotubes (MWCNTs) and asbestos (crocidolite) and found to be approximately 0.0135 and 0.066%, respectively, in the case of 24-h treatment of normal human bronchial epithelia (NHBE) cells. Using these GI
50
concentrations, NHBE cells were then treated with MWCNTs and asbestos for 6 and 24 h, followed by a DNA microarray analysis. Among 31,647 genes, 1,201 and 1,252 were up-regulated by both asbestos and MWCNTs after 6 and 24 h of exposure, respectively. Meanwhile, 1,977 and 1,542 genes were down-regulated by both asbestos and MWNCTs after 6 and 24 h of exposure, respectively. In particular, the asbestos and MWCNTs both induced an over twofold up- and down-regulated expression of 12 mesothelioma-related genes and 22 lung cancer-related genes when compared with the negative control. Plus, the genes induced by the MWCNT exposure were expressed in the brain, lungs, epithelium, liver, and colon.</description><subject>Asbestos</subject><subject>Asbestos, Crocidolite - toxicity</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bronchi - drug effects</subject><subject>Carbon</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Comparative studies</subject><subject>Environmental Health</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genomics</subject><subject>Humans</subject><subject>Inorganic Compounds</subject><subject>Nanotubes</subject><subject>Nanotubes, Carbon - toxicity</subject><subject>Occupational Medicine/Industrial Medicine</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pharmacology/Toxicology</subject><subject>Respiratory Mucosa - drug effects</subject><subject>Toxicogenetics</subject><subject>Toxicology</subject><issn>0340-5761</issn><issn>1432-0738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kMtOwzAQRS0EoqXwAWxQxKosDONHYnuJKl4ShU0Ry8hxnSpVEhc7EfD3uEoBCYmV5Ttn7sxchE4JXBIAcRUAKHAMhGAQAnC2h8aEMxp_TO6jMTAOOBUZGaGjENYAhErFDtGIUogqpGM0X7iPyriVbV1TmcS4ZqN9FVybuDJp-rqr8Luu68RoX0Sx1a3r-sKGZDp_nT0twkWi22WiQ5Q6F47RQanrYE927wS93N4sZvf48fnuYXb9iA3PVIdVVsq0oLYErYkuUpYyUCk1UklbSpEqURCpFCw5KTmhRIIUWkFpoJBgacomaDr4brx76-PovKmCsXWtW-v6kMd0pIzH8y16_gddu963cbtcUQWEc8IiRAbIeBeCt2W-8VWj_Wd02pqJfIg6j1Hn26jzLPac7Yz7orHLn47vbCNAByDEUruy_nfy_65f1EqGyA</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Kim, Jin Sik</creator><creator>Song, Kyung Seuk</creator><creator>Lee, Jin Kyu</creator><creator>Choi, Young C.</creator><creator>Bang, In Seok</creator><creator>Kang, Chang Soo</creator><creator>Yu, Il Je</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T2</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>MBDVC</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7ST</scope><scope>7TV</scope><scope>SOI</scope></search><sort><creationdate>20120401</creationdate><title>Toxicogenomic comparison of multi-wall carbon nanotubes (MWCNTs) and asbestos</title><author>Kim, Jin Sik ; Song, Kyung Seuk ; Lee, Jin Kyu ; Choi, Young C. ; Bang, In Seok ; Kang, Chang Soo ; Yu, Il Je</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-96f85b2ef0aa1ab53530952c898ef87597b18990d41f41218087a90fc0b80e253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Asbestos</topic><topic>Asbestos, Crocidolite - toxicity</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bronchi - drug effects</topic><topic>Carbon</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Comparative studies</topic><topic>Environmental Health</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genomics</topic><topic>Humans</topic><topic>Inorganic Compounds</topic><topic>Nanotubes</topic><topic>Nanotubes, Carbon - toxicity</topic><topic>Occupational Medicine/Industrial Medicine</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pharmacology/Toxicology</topic><topic>Respiratory Mucosa - drug effects</topic><topic>Toxicogenetics</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jin Sik</creatorcontrib><creatorcontrib>Song, Kyung Seuk</creatorcontrib><creatorcontrib>Lee, Jin Kyu</creatorcontrib><creatorcontrib>Choi, Young C.</creatorcontrib><creatorcontrib>Bang, In Seok</creatorcontrib><creatorcontrib>Kang, Chang Soo</creatorcontrib><creatorcontrib>Yu, Il Je</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database (ProQuest)</collection><collection>Research Library (Corporate)</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Archives of toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jin Sik</au><au>Song, Kyung Seuk</au><au>Lee, Jin Kyu</au><au>Choi, Young C.</au><au>Bang, In Seok</au><au>Kang, Chang Soo</au><au>Yu, Il Je</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxicogenomic comparison of multi-wall carbon nanotubes (MWCNTs) and asbestos</atitle><jtitle>Archives of toxicology</jtitle><stitle>Arch Toxicol</stitle><addtitle>Arch Toxicol</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>86</volume><issue>4</issue><spage>553</spage><epage>562</epage><pages>553-562</pages><issn>0340-5761</issn><eissn>1432-0738</eissn><abstract>Carbon nanotubes (CNTs) have specific properties, including electrical and thermal conductivity, great strength, and rigidity, that allow them to be used in many fields. However, this increasing contact with humans and the environment is also raising health and safety concerns. Thus, research on the safety of CNTs has attracted much interest, including a comparison of the toxic effects of asbestos and carbon nanotubes, due to their physical similarity of a high aspect ratio (length/diameter). Nonetheless, there has not yet been a toxicogenomic comparison. Therefore, to examine toxicogenomic effects, the 50% growth inhibition (GI
50
) concentration was determined for multi-wall carbon nanotubes (MWCNTs) and asbestos (crocidolite) and found to be approximately 0.0135 and 0.066%, respectively, in the case of 24-h treatment of normal human bronchial epithelia (NHBE) cells. Using these GI
50
concentrations, NHBE cells were then treated with MWCNTs and asbestos for 6 and 24 h, followed by a DNA microarray analysis. Among 31,647 genes, 1,201 and 1,252 were up-regulated by both asbestos and MWCNTs after 6 and 24 h of exposure, respectively. Meanwhile, 1,977 and 1,542 genes were down-regulated by both asbestos and MWNCTs after 6 and 24 h of exposure, respectively. In particular, the asbestos and MWCNTs both induced an over twofold up- and down-regulated expression of 12 mesothelioma-related genes and 22 lung cancer-related genes when compared with the negative control. Plus, the genes induced by the MWCNT exposure were expressed in the brain, lungs, epithelium, liver, and colon.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22076105</pmid><doi>10.1007/s00204-011-0770-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0340-5761 |
| ispartof | Archives of toxicology, 2012-04, Vol.86 (4), p.553-562 |
| issn | 0340-5761 1432-0738 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1008834045 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Asbestos Asbestos, Crocidolite - toxicity Biomedical and Life Sciences Biomedicine Bronchi - drug effects Carbon Cell Line Cell Survival - drug effects Comparative studies Environmental Health Gene Expression Profiling Gene Expression Regulation - drug effects Genomics Humans Inorganic Compounds Nanotubes Nanotubes, Carbon - toxicity Occupational Medicine/Industrial Medicine Oligonucleotide Array Sequence Analysis Pharmacology/Toxicology Respiratory Mucosa - drug effects Toxicogenetics Toxicology |
| title | Toxicogenomic comparison of multi-wall carbon nanotubes (MWCNTs) and asbestos |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T07%3A05%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Toxicogenomic%20comparison%20of%20multi-wall%20carbon%20nanotubes%20(MWCNTs)%20and%20asbestos&rft.jtitle=Archives%20of%20toxicology&rft.au=Kim,%20Jin%20Sik&rft.date=2012-04-01&rft.volume=86&rft.issue=4&rft.spage=553&rft.epage=562&rft.pages=553-562&rft.issn=0340-5761&rft.eissn=1432-0738&rft_id=info:doi/10.1007/s00204-011-0770-6&rft_dat=%3Cproquest_cross%3E2613421031%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=929014413&rft_id=info:pmid/22076105&rfr_iscdi=true |