Babesia ovis as the main causative agent of sheep babesiosis in Iran
Babesiosis is a haemoparasitic disease with high economical losses in livestock industry worldwide. The early diagnosis and successful therapy of babesiosis belong to the key steps of control and health management of livestock. Ethanol-fixed blood samples of 400 sheep were analyzed for Babesia infec...
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| Veröffentlicht in: | Parasitology research (1987) 2012-04, Vol.110 (4), p.1531-1536 |
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| creator | Ranjbar-Bahadori, Shahrokh Eckert, Brigitte Omidian, Zahra Shirazi, Nastran Sadr Shayan, Parviz |
| description | Babesiosis is a haemoparasitic disease with high economical losses in livestock industry worldwide. The early diagnosis and successful therapy of babesiosis belong to the key steps of control and health management of livestock. Ethanol-fixed blood samples of 400 sheep were analyzed for
Babesia
infection. Reverse line blot (RLB) was established specifically for
Theileria lestoquardi
,
Theileria
(China 1),
Theileria
(China 2),
Theileria ovis
,
Theileria separata
,
Babesia ovis
,
Babesia motasi
,
Babesia crassa
, and
Babesia
(Lintan). The DNA was extracted from the ethanol-fixed blood samples and amplified with a common primer pair derived from 18S rRNA gene, amplifying both
Theileria
spp. as well as
Babesia
spp. Regarding the differences in the length of nucleotide sequences of the polymerase chain reaction (PCR) products obtained from
Theileria
spp. and
Babesia
spp., the PCR products derived from
Babesia
spp. were out screened and analyzed by RLB. The RLB analysis showed that 28 samples within the 400 blood samples were
B. ovis
positive. No
B. motasi
,
B. crassa
, or
Babesia
(Lintan) could be detected. The sequence analysis of one PCR product as a representative for other
B. ovis-
positive PCR products confirmed the results of RLB. Our results and the results of other investigators showed that
B. ovis
could be considered as a main causative agent of sheep babesiosis in Iran. Furthermore, our results also showed that RLB can be used as a reliable method for a simultaneous differentiation of
Theileria
and
Babesia
species from each other. |
| doi_str_mv | 10.1007/s00436-011-2658-z |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1008827071</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A305995994</galeid><sourcerecordid>A305995994</sourcerecordid><originalsourceid>FETCH-LOGICAL-c555t-aeb0bc125377cb97cc2830da76d184727c4b73dd80a8ac4b85f56d7b15c0fc183</originalsourceid><addsrcrecordid>eNp9kV1rFTEQhoMo9lj9Ad5IQITebJuPzSZ7WWvVQqE3eh1ms9nTlP04ZnYL9tc7xz0qQikJZMg8b97wDmNvpTiVQtgzFKLUVSGkLFRlXPHwjG1kqVUha2Oes42oqaauPmKvEO-EkLYqy5fsSMnamsqVG_bpIzQRE_DpPiEH5PNt5AOkkQdYEOZ0Hzls4zjzqeN4G-OON78VExJP2FWG8TV70UGP8c3hPGbfP19-u_haXN98ubo4vy6CMWYuIDaiCVIZbW1oahuCclq0YKtWutIqG8rG6rZ1AhxQ7UxnqtY20gTRBen0MTtZ393l6ccScfZDwhD7HsY4LegpFOeUFVYS-n5Ft9BHn8ZumjOEPe7PtTB1Tbsk6vQRilYbhxSmMXaJ7v8TyFUQ8oSYY-d3OQ2Qf5L33t76dSaeUvf7mfgH0rw7_Hpphtj-VfwZAgEfDgBggL6jREPCf5ypaquVJk6tHFJr3Mbs76Ylj5T4E-6_AMAmodA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1008827071</pqid></control><display><type>article</type><title>Babesia ovis as the main causative agent of sheep babesiosis in Iran</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Ranjbar-Bahadori, Shahrokh ; Eckert, Brigitte ; Omidian, Zahra ; Shirazi, Nastran Sadr ; Shayan, Parviz</creator><creatorcontrib>Ranjbar-Bahadori, Shahrokh ; Eckert, Brigitte ; Omidian, Zahra ; Shirazi, Nastran Sadr ; Shayan, Parviz</creatorcontrib><description>Babesiosis is a haemoparasitic disease with high economical losses in livestock industry worldwide. The early diagnosis and successful therapy of babesiosis belong to the key steps of control and health management of livestock. Ethanol-fixed blood samples of 400 sheep were analyzed for
Babesia
infection. Reverse line blot (RLB) was established specifically for
Theileria lestoquardi
,
Theileria
(China 1),
Theileria
(China 2),
Theileria ovis
,
Theileria separata
,
Babesia ovis
,
Babesia motasi
,
Babesia crassa
, and
Babesia
(Lintan). The DNA was extracted from the ethanol-fixed blood samples and amplified with a common primer pair derived from 18S rRNA gene, amplifying both
Theileria
spp. as well as
Babesia
spp. Regarding the differences in the length of nucleotide sequences of the polymerase chain reaction (PCR) products obtained from
Theileria
spp. and
Babesia
spp., the PCR products derived from
Babesia
spp. were out screened and analyzed by RLB. The RLB analysis showed that 28 samples within the 400 blood samples were
B. ovis
positive. No
B. motasi
,
B. crassa
, or
Babesia
(Lintan) could be detected. The sequence analysis of one PCR product as a representative for other
B. ovis-
positive PCR products confirmed the results of RLB. Our results and the results of other investigators showed that
B. ovis
could be considered as a main causative agent of sheep babesiosis in Iran. Furthermore, our results also showed that RLB can be used as a reliable method for a simultaneous differentiation of
Theileria
and
Babesia
species from each other.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-011-2658-z</identifier><identifier>PMID: 21975684</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Babesia ; Babesia - classification ; Babesia - isolation & purification ; Babesia - pathogenicity ; Babesiosis ; Babesiosis - diagnosis ; Babesiosis - epidemiology ; Babesiosis - parasitology ; Babesiosis - veterinary ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Causes of ; DNA Primers - genetics ; DNA, Protozoan - analysis ; DNA, Protozoan - genetics ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Health aspects ; Host-parasite relationships ; Immunology ; Invertebrates ; Iran - epidemiology ; Mammalia ; Medical Microbiology ; Microbiology ; Original Paper ; Physiological aspects ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; RNA, Ribosomal, 18S - genetics ; RNA, Ribosomal, 18S - isolation & purification ; Sheep ; Sheep - genetics ; Sheep - parasitology ; Sheep Diseases - diagnosis ; Sheep Diseases - epidemiology ; Sheep Diseases - parasitology ; Theileria - classification ; Theileria - isolation & purification ; Theileria - pathogenicity ; Theileriasis - diagnosis ; Theileriasis - epidemiology ; Theileriasis - parasitology ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><ispartof>Parasitology research (1987), 2012-04, Vol.110 (4), p.1531-1536</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2012 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-aeb0bc125377cb97cc2830da76d184727c4b73dd80a8ac4b85f56d7b15c0fc183</citedby><cites>FETCH-LOGICAL-c555t-aeb0bc125377cb97cc2830da76d184727c4b73dd80a8ac4b85f56d7b15c0fc183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-011-2658-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-011-2658-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25697323$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21975684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ranjbar-Bahadori, Shahrokh</creatorcontrib><creatorcontrib>Eckert, Brigitte</creatorcontrib><creatorcontrib>Omidian, Zahra</creatorcontrib><creatorcontrib>Shirazi, Nastran Sadr</creatorcontrib><creatorcontrib>Shayan, Parviz</creatorcontrib><title>Babesia ovis as the main causative agent of sheep babesiosis in Iran</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Babesiosis is a haemoparasitic disease with high economical losses in livestock industry worldwide. The early diagnosis and successful therapy of babesiosis belong to the key steps of control and health management of livestock. Ethanol-fixed blood samples of 400 sheep were analyzed for
Babesia
infection. Reverse line blot (RLB) was established specifically for
Theileria lestoquardi
,
Theileria
(China 1),
Theileria
(China 2),
Theileria ovis
,
Theileria separata
,
Babesia ovis
,
Babesia motasi
,
Babesia crassa
, and
Babesia
(Lintan). The DNA was extracted from the ethanol-fixed blood samples and amplified with a common primer pair derived from 18S rRNA gene, amplifying both
Theileria
spp. as well as
Babesia
spp. Regarding the differences in the length of nucleotide sequences of the polymerase chain reaction (PCR) products obtained from
Theileria
spp. and
Babesia
spp., the PCR products derived from
Babesia
spp. were out screened and analyzed by RLB. The RLB analysis showed that 28 samples within the 400 blood samples were
B. ovis
positive. No
B. motasi
,
B. crassa
, or
Babesia
(Lintan) could be detected. The sequence analysis of one PCR product as a representative for other
B. ovis-
positive PCR products confirmed the results of RLB. Our results and the results of other investigators showed that
B. ovis
could be considered as a main causative agent of sheep babesiosis in Iran. Furthermore, our results also showed that RLB can be used as a reliable method for a simultaneous differentiation of
Theileria
and
Babesia
species from each other.</description><subject>Animals</subject><subject>Babesia</subject><subject>Babesia - classification</subject><subject>Babesia - isolation & purification</subject><subject>Babesia - pathogenicity</subject><subject>Babesiosis</subject><subject>Babesiosis - diagnosis</subject><subject>Babesiosis - epidemiology</subject><subject>Babesiosis - parasitology</subject><subject>Babesiosis - veterinary</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Causes of</subject><subject>DNA Primers - genetics</subject><subject>DNA, Protozoan - analysis</subject><subject>DNA, Protozoan - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Health aspects</subject><subject>Host-parasite relationships</subject><subject>Immunology</subject><subject>Invertebrates</subject><subject>Iran - epidemiology</subject><subject>Mammalia</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Physiological aspects</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>RNA, Ribosomal, 18S - isolation & purification</subject><subject>Sheep</subject><subject>Sheep - genetics</subject><subject>Sheep - parasitology</subject><subject>Sheep Diseases - diagnosis</subject><subject>Sheep Diseases - epidemiology</subject><subject>Sheep Diseases - parasitology</subject><subject>Theileria - classification</subject><subject>Theileria - isolation & purification</subject><subject>Theileria - pathogenicity</subject><subject>Theileriasis - diagnosis</subject><subject>Theileriasis - epidemiology</subject><subject>Theileriasis - parasitology</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV1rFTEQhoMo9lj9Ad5IQITebJuPzSZ7WWvVQqE3eh1ms9nTlP04ZnYL9tc7xz0qQikJZMg8b97wDmNvpTiVQtgzFKLUVSGkLFRlXPHwjG1kqVUha2Oes42oqaauPmKvEO-EkLYqy5fsSMnamsqVG_bpIzQRE_DpPiEH5PNt5AOkkQdYEOZ0Hzls4zjzqeN4G-OON78VExJP2FWG8TV70UGP8c3hPGbfP19-u_haXN98ubo4vy6CMWYuIDaiCVIZbW1oahuCclq0YKtWutIqG8rG6rZ1AhxQ7UxnqtY20gTRBen0MTtZ393l6ccScfZDwhD7HsY4LegpFOeUFVYS-n5Ft9BHn8ZumjOEPe7PtTB1Tbsk6vQRilYbhxSmMXaJ7v8TyFUQ8oSYY-d3OQ2Qf5L33t76dSaeUvf7mfgH0rw7_Hpphtj-VfwZAgEfDgBggL6jREPCf5ypaquVJk6tHFJr3Mbs76Ylj5T4E-6_AMAmodA</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Ranjbar-Bahadori, Shahrokh</creator><creator>Eckert, Brigitte</creator><creator>Omidian, Zahra</creator><creator>Shirazi, Nastran Sadr</creator><creator>Shayan, Parviz</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120401</creationdate><title>Babesia ovis as the main causative agent of sheep babesiosis in Iran</title><author>Ranjbar-Bahadori, Shahrokh ; Eckert, Brigitte ; Omidian, Zahra ; Shirazi, Nastran Sadr ; Shayan, Parviz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-aeb0bc125377cb97cc2830da76d184727c4b73dd80a8ac4b85f56d7b15c0fc183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Babesia</topic><topic>Babesia - classification</topic><topic>Babesia - isolation & purification</topic><topic>Babesia - pathogenicity</topic><topic>Babesiosis</topic><topic>Babesiosis - diagnosis</topic><topic>Babesiosis - epidemiology</topic><topic>Babesiosis - parasitology</topic><topic>Babesiosis - veterinary</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Causes of</topic><topic>DNA Primers - genetics</topic><topic>DNA, Protozoan - analysis</topic><topic>DNA, Protozoan - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Health aspects</topic><topic>Host-parasite relationships</topic><topic>Immunology</topic><topic>Invertebrates</topic><topic>Iran - epidemiology</topic><topic>Mammalia</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Physiological aspects</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>RNA, Ribosomal, 18S - isolation & purification</topic><topic>Sheep</topic><topic>Sheep - genetics</topic><topic>Sheep - parasitology</topic><topic>Sheep Diseases - diagnosis</topic><topic>Sheep Diseases - epidemiology</topic><topic>Sheep Diseases - parasitology</topic><topic>Theileria - classification</topic><topic>Theileria - isolation & purification</topic><topic>Theileria - pathogenicity</topic><topic>Theileriasis - diagnosis</topic><topic>Theileriasis - epidemiology</topic><topic>Theileriasis - parasitology</topic><topic>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ranjbar-Bahadori, Shahrokh</creatorcontrib><creatorcontrib>Eckert, Brigitte</creatorcontrib><creatorcontrib>Omidian, Zahra</creatorcontrib><creatorcontrib>Shirazi, Nastran Sadr</creatorcontrib><creatorcontrib>Shayan, Parviz</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ranjbar-Bahadori, Shahrokh</au><au>Eckert, Brigitte</au><au>Omidian, Zahra</au><au>Shirazi, Nastran Sadr</au><au>Shayan, Parviz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Babesia ovis as the main causative agent of sheep babesiosis in Iran</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>110</volume><issue>4</issue><spage>1531</spage><epage>1536</epage><pages>1531-1536</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>Babesiosis is a haemoparasitic disease with high economical losses in livestock industry worldwide. The early diagnosis and successful therapy of babesiosis belong to the key steps of control and health management of livestock. Ethanol-fixed blood samples of 400 sheep were analyzed for
Babesia
infection. Reverse line blot (RLB) was established specifically for
Theileria lestoquardi
,
Theileria
(China 1),
Theileria
(China 2),
Theileria ovis
,
Theileria separata
,
Babesia ovis
,
Babesia motasi
,
Babesia crassa
, and
Babesia
(Lintan). The DNA was extracted from the ethanol-fixed blood samples and amplified with a common primer pair derived from 18S rRNA gene, amplifying both
Theileria
spp. as well as
Babesia
spp. Regarding the differences in the length of nucleotide sequences of the polymerase chain reaction (PCR) products obtained from
Theileria
spp. and
Babesia
spp., the PCR products derived from
Babesia
spp. were out screened and analyzed by RLB. The RLB analysis showed that 28 samples within the 400 blood samples were
B. ovis
positive. No
B. motasi
,
B. crassa
, or
Babesia
(Lintan) could be detected. The sequence analysis of one PCR product as a representative for other
B. ovis-
positive PCR products confirmed the results of RLB. Our results and the results of other investigators showed that
B. ovis
could be considered as a main causative agent of sheep babesiosis in Iran. Furthermore, our results also showed that RLB can be used as a reliable method for a simultaneous differentiation of
Theileria
and
Babesia
species from each other.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21975684</pmid><doi>10.1007/s00436-011-2658-z</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2012-04, Vol.110 (4), p.1531-1536 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1008827071 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Babesia Babesia - classification Babesia - isolation & purification Babesia - pathogenicity Babesiosis Babesiosis - diagnosis Babesiosis - epidemiology Babesiosis - parasitology Babesiosis - veterinary Biological and medical sciences Biomedical and Life Sciences Biomedicine Causes of DNA Primers - genetics DNA, Protozoan - analysis DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Health aspects Host-parasite relationships Immunology Invertebrates Iran - epidemiology Mammalia Medical Microbiology Microbiology Original Paper Physiological aspects Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary RNA, Ribosomal, 18S - genetics RNA, Ribosomal, 18S - isolation & purification Sheep Sheep - genetics Sheep - parasitology Sheep Diseases - diagnosis Sheep Diseases - epidemiology Sheep Diseases - parasitology Theileria - classification Theileria - isolation & purification Theileria - pathogenicity Theileriasis - diagnosis Theileriasis - epidemiology Theileriasis - parasitology Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution |
| title | Babesia ovis as the main causative agent of sheep babesiosis in Iran |
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