Phylogenetic analyses of pandemic influenza A (H1N1) virus in university students at Tobetsu, Hokkaido, Japan
ABSTRACT Pandemic influenza H1N1 virus (A[H1N1]pdm09) emerged in 2009. To determine the phylogeography of A(H1N1)pdm09 in a single population, 70 strains of the virus were isolated from university students or trainee doctors at Tobetsu, Hokkaido, Japan, between September and December 2009. The nucle...
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| Veröffentlicht in: | Microbiology and immunology 2012-04, Vol.56 (4), p.273-279 |
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| container_title | Microbiology and immunology |
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| creator | Inoue, Emi Ieko, Masahiro Takahashi, Nobuhiko Osawa, Yoshiaki Okazaki, Katsunori |
| description | ABSTRACT
Pandemic influenza H1N1 virus (A[H1N1]pdm09) emerged in 2009. To determine the phylogeography of A(H1N1)pdm09 in a single population, 70 strains of the virus were isolated from university students or trainee doctors at Tobetsu, Hokkaido, Japan, between September and December 2009. The nucleotide sequences of the HA1 region of the HA genes and described phylogenetic relationships of the strains circulating among them were analyzed. It was found that the 70 isolates could be phylogenetically separated into three groups and that two epidemics were caused by different groups of the virus. The three groups were also distinguishable from each other by three amino acid changes: A197T, S203T and Q293H. The substitution of S203T, which is located in the antigenic site, suggests antigenic drift of the virus. |
| doi_str_mv | 10.1111/j.1348-0421.2012.00432.x |
| format | Article |
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Pandemic influenza H1N1 virus (A[H1N1]pdm09) emerged in 2009. To determine the phylogeography of A(H1N1)pdm09 in a single population, 70 strains of the virus were isolated from university students or trainee doctors at Tobetsu, Hokkaido, Japan, between September and December 2009. The nucleotide sequences of the HA1 region of the HA genes and described phylogenetic relationships of the strains circulating among them were analyzed. It was found that the 70 isolates could be phylogenetically separated into three groups and that two epidemics were caused by different groups of the virus. The three groups were also distinguishable from each other by three amino acid changes: A197T, S203T and Q293H. The substitution of S203T, which is located in the antigenic site, suggests antigenic drift of the virus.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.2012.00432.x</identifier><identifier>PMID: 22500934</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Amino Acid Substitution ; Cluster Analysis ; epidemic ; Genotype ; Hemagglutinin Glycoproteins, Influenza Virus - genetics ; Humans ; influenza ; Influenza A Virus, H1N1 Subtype - classification ; Influenza A Virus, H1N1 Subtype - genetics ; Influenza A Virus, H1N1 Subtype - isolation & purification ; Influenza, Human - epidemiology ; Influenza, Human - virology ; Japan - epidemiology ; Molecular Epidemiology ; Molecular Sequence Data ; pandemic (H1N1) 2009 ; Pandemics ; phylogenetic analyses ; Phylogeny ; Polymorphism, Genetic ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Students ; Universities</subject><ispartof>Microbiology and immunology, 2012-04, Vol.56 (4), p.273-279</ispartof><rights>2012 The Societies and Blackwell Publishing Asia Pty Ltd</rights><rights>2012 The Societies and Blackwell Publishing Asia Pty Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4802-29f193690fa2bc7cdab5bc6c5b2eae4f6bf435a4857eae4b53edff1f90ba29163</citedby><cites>FETCH-LOGICAL-c4802-29f193690fa2bc7cdab5bc6c5b2eae4f6bf435a4857eae4b53edff1f90ba29163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1348-0421.2012.00432.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1348-0421.2012.00432.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22500934$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, Emi</creatorcontrib><creatorcontrib>Ieko, Masahiro</creatorcontrib><creatorcontrib>Takahashi, Nobuhiko</creatorcontrib><creatorcontrib>Osawa, Yoshiaki</creatorcontrib><creatorcontrib>Okazaki, Katsunori</creatorcontrib><title>Phylogenetic analyses of pandemic influenza A (H1N1) virus in university students at Tobetsu, Hokkaido, Japan</title><title>Microbiology and immunology</title><addtitle>Microbiol Immunol</addtitle><description>ABSTRACT
Pandemic influenza H1N1 virus (A[H1N1]pdm09) emerged in 2009. To determine the phylogeography of A(H1N1)pdm09 in a single population, 70 strains of the virus were isolated from university students or trainee doctors at Tobetsu, Hokkaido, Japan, between September and December 2009. The nucleotide sequences of the HA1 region of the HA genes and described phylogenetic relationships of the strains circulating among them were analyzed. It was found that the 70 isolates could be phylogenetically separated into three groups and that two epidemics were caused by different groups of the virus. The three groups were also distinguishable from each other by three amino acid changes: A197T, S203T and Q293H. The substitution of S203T, which is located in the antigenic site, suggests antigenic drift of the virus.</description><subject>Amino Acid Substitution</subject><subject>Cluster Analysis</subject><subject>epidemic</subject><subject>Genotype</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - genetics</subject><subject>Humans</subject><subject>influenza</subject><subject>Influenza A Virus, H1N1 Subtype - classification</subject><subject>Influenza A Virus, H1N1 Subtype - genetics</subject><subject>Influenza A Virus, H1N1 Subtype - isolation & purification</subject><subject>Influenza, Human - epidemiology</subject><subject>Influenza, Human - virology</subject><subject>Japan - epidemiology</subject><subject>Molecular Epidemiology</subject><subject>Molecular Sequence Data</subject><subject>pandemic (H1N1) 2009</subject><subject>Pandemics</subject><subject>phylogenetic analyses</subject><subject>Phylogeny</subject><subject>Polymorphism, Genetic</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Students</subject><subject>Universities</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtu1DAYhS0EokPhFZCXRWpSXxNHYlNVtNMrrVRgadnJb_BMLkOclAlPj8OUWeONL_93jqUPIUxJSuM6WaWUC5UQwWjKCGUpIYKzdPsCLfaDl2hBuJKJzAg5QG9CWBHCcqbEa3TAmCSk4GKBmvsfU919hxYGX2LTmnoKEHDn8Ma0FTTx0beuHqH9bfApPlrSO_oBP_l-DHGAx9Y_QR_8MOEwjBW0Q8BmwI-dhSGMx3jZrdfGV90xvjKx8C165Uwd4N3zfoi-nH96PFsmN58vLs9Ob5JSKMISVjha8KwgzjBb5mVlrLRlVkrLwIBwmXWCSyOUzOe7lRwq56griDWsoBk_REe73k3f_RwhDLrxoYS6Ni10Y9CUEFpIpTiNqNqhZd-F0IPTm943pp8ipGfZeqVnp3p2qmfZ-q9svY3R98-_jLaBah_8ZzcCH3fAL1_D9N_F-vbyNh5iPNnFfRhgu4-bfq2znOdSf7u70GT5lT-o8yt9zf8AzIqdEQ</recordid><startdate>201204</startdate><enddate>201204</enddate><creator>Inoue, Emi</creator><creator>Ieko, Masahiro</creator><creator>Takahashi, Nobuhiko</creator><creator>Osawa, Yoshiaki</creator><creator>Okazaki, Katsunori</creator><general>Blackwell Publishing Asia</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201204</creationdate><title>Phylogenetic analyses of pandemic influenza A (H1N1) virus in university students at Tobetsu, Hokkaido, Japan</title><author>Inoue, Emi ; Ieko, Masahiro ; Takahashi, Nobuhiko ; Osawa, Yoshiaki ; Okazaki, Katsunori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4802-29f193690fa2bc7cdab5bc6c5b2eae4f6bf435a4857eae4b53edff1f90ba29163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Substitution</topic><topic>Cluster Analysis</topic><topic>epidemic</topic><topic>Genotype</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - genetics</topic><topic>Humans</topic><topic>influenza</topic><topic>Influenza A Virus, H1N1 Subtype - classification</topic><topic>Influenza A Virus, H1N1 Subtype - genetics</topic><topic>Influenza A Virus, H1N1 Subtype - isolation & purification</topic><topic>Influenza, Human - epidemiology</topic><topic>Influenza, Human - virology</topic><topic>Japan - epidemiology</topic><topic>Molecular Epidemiology</topic><topic>Molecular Sequence Data</topic><topic>pandemic (H1N1) 2009</topic><topic>Pandemics</topic><topic>phylogenetic analyses</topic><topic>Phylogeny</topic><topic>Polymorphism, Genetic</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Students</topic><topic>Universities</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inoue, Emi</creatorcontrib><creatorcontrib>Ieko, Masahiro</creatorcontrib><creatorcontrib>Takahashi, Nobuhiko</creatorcontrib><creatorcontrib>Osawa, Yoshiaki</creatorcontrib><creatorcontrib>Okazaki, Katsunori</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, Emi</au><au>Ieko, Masahiro</au><au>Takahashi, Nobuhiko</au><au>Osawa, Yoshiaki</au><au>Okazaki, Katsunori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic analyses of pandemic influenza A (H1N1) virus in university students at Tobetsu, Hokkaido, Japan</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiol Immunol</addtitle><date>2012-04</date><risdate>2012</risdate><volume>56</volume><issue>4</issue><spage>273</spage><epage>279</epage><pages>273-279</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>ABSTRACT
Pandemic influenza H1N1 virus (A[H1N1]pdm09) emerged in 2009. To determine the phylogeography of A(H1N1)pdm09 in a single population, 70 strains of the virus were isolated from university students or trainee doctors at Tobetsu, Hokkaido, Japan, between September and December 2009. The nucleotide sequences of the HA1 region of the HA genes and described phylogenetic relationships of the strains circulating among them were analyzed. It was found that the 70 isolates could be phylogenetically separated into three groups and that two epidemics were caused by different groups of the virus. The three groups were also distinguishable from each other by three amino acid changes: A197T, S203T and Q293H. The substitution of S203T, which is located in the antigenic site, suggests antigenic drift of the virus.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>22500934</pmid><doi>10.1111/j.1348-0421.2012.00432.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Substitution Cluster Analysis epidemic Genotype Hemagglutinin Glycoproteins, Influenza Virus - genetics Humans influenza Influenza A Virus, H1N1 Subtype - classification Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - isolation & purification Influenza, Human - epidemiology Influenza, Human - virology Japan - epidemiology Molecular Epidemiology Molecular Sequence Data pandemic (H1N1) 2009 Pandemics phylogenetic analyses Phylogeny Polymorphism, Genetic RNA, Viral - genetics Sequence Analysis, DNA Students Universities |
| title | Phylogenetic analyses of pandemic influenza A (H1N1) virus in university students at Tobetsu, Hokkaido, Japan |
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