Proteomic sample preparation by microdialysis: Easy, speedy, and nonselective

Microdialysis tools have been developed for parallelized medium exchange designated for sample volumes from 10 to 100μl, compatible with the microplate format, and guaranteeing maximum recoveries without selectivity. These tools are applicable to both protein and peptide analysis. Moreover, they may...

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Veröffentlicht in:	Analytical biochemistry 2012-05, Vol.424 (2), p.184-186
Hauptverfasser:	Maischak, Heiko, Tautkus, Bärbel, Kreusch, Stefan, Rhode, Heidrun
Format:	Artikel
Sprache:	eng
Schlagworte:	containers High-Throughput Screening Assays Humans Medium exchange Micro dialysis microdialysis Microdialysis - instrumentation Microdialysis - methods Peptides - analysis Protein Binding Protein desalting Proteomics Sample preparation Serum Albumin - analysis
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Microdialysis tools have been developed for parallelized medium exchange designated for sample volumes from 10 to 100μl, compatible with the microplate format, and guaranteeing maximum recoveries without selectivity. These tools are applicable to both protein and peptide analysis. Moreover, they may be used for binding studies as well as for reconcentration and as unique sample containers for complex operating sequences allowing contemporaneous processing and high throughput.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2012.01.037