Recombinant derivatives of clostridial neurotoxins as delivery vehicles for proteins and small organic molecules
Clostridial neurotoxins are the most powerful toxins known. Nevertheless, derivatives of these toxins may find broad applications both in science and medicine because of their unique abilities to recognize neurons and deliver small and large molecules into them. In this paper we describe the constru...
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| Veröffentlicht in: | Journal of Protein Chemistry 2000-11, Vol.19 (8), p.699-707 |
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| container_title | Journal of Protein Chemistry |
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| creator | Zdanovskaia, M V Los, G Zdanovsky, A G |
| description | Clostridial neurotoxins are the most powerful toxins known. Nevertheless, derivatives of these toxins may find broad applications both in science and medicine because of their unique abilities to recognize neurons and deliver small and large molecules into them. In this paper we describe the construction of two types of such derivatives. Proteins belonging to the first class were designed to allow direct conjugation with one or few molecules of interest. Proteins belonging to the second class contain biotin residue and therefore could be easily connected to streptavidin loaded with multiple molecules of interest. Only C-terminal regions of neurotoxin heavy chains were incorporated in the structure of recombinant proteins. Nevertheless, recombinant proteins were found to be able to recognize specific neuronal receptors and target model molecules to rat synaptosomes and human neuroblastoma cells. |
| doi_str_mv | 10.1023/A:1007164619921 |
| format | Article |
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Nevertheless, derivatives of these toxins may find broad applications both in science and medicine because of their unique abilities to recognize neurons and deliver small and large molecules into them. In this paper we describe the construction of two types of such derivatives. Proteins belonging to the first class were designed to allow direct conjugation with one or few molecules of interest. Proteins belonging to the second class contain biotin residue and therefore could be easily connected to streptavidin loaded with multiple molecules of interest. Only C-terminal regions of neurotoxin heavy chains were incorporated in the structure of recombinant proteins. Nevertheless, recombinant proteins were found to be able to recognize specific neuronal receptors and target model molecules to rat synaptosomes and human neuroblastoma cells.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biotin</subject><subject>Botulinum Toxins - administration & dosage</subject><subject>Botulinum Toxins - genetics</subject><subject>Conjugation</subject><subject>DNA Primers</subject><subject>Drug Carriers</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli - genetics</subject><subject>Humans</subject><subject>Molecular biology</subject><subject>Neurobiology</subject><subject>Neuroblastoma</subject><subject>Neuroblasts</subject><subject>Neurotoxins</subject><subject>Organic Chemicals - administration & dosage</subject><subject>Organic chemistry</subject><subject>Proteins</subject><subject>Proteins - administration & dosage</subject><subject>Rats</subject><subject>Recombinant Proteins - administration & dosage</subject><subject>Recombinant Proteins - 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| subjects | Animals Base Sequence Biotin Botulinum Toxins - administration & dosage Botulinum Toxins - genetics Conjugation DNA Primers Drug Carriers Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Humans Molecular biology Neurobiology Neuroblastoma Neuroblasts Neurotoxins Organic Chemicals - administration & dosage Organic chemistry Proteins Proteins - administration & dosage Rats Recombinant Proteins - administration & dosage Recombinant Proteins - genetics Streptavidin Synaptosomes Toxins Tumor Cells, Cultured |
| title | Recombinant derivatives of clostridial neurotoxins as delivery vehicles for proteins and small organic molecules |
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