Novel PCR primers for detection of genetically diverse virulent Agrobacterium tumefaciens biovar 1 strains
Novel PCR primers were developed to amplify a 243-bp fragment of an intergenic region between gene 5 and tms 2 on the T-DNA of Agrobacterium tumefaciens biovar 1 . These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did not generate e...
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| Veröffentlicht in: | Journal of general plant pathology : JGPP 2012-03, Vol.78 (2), p.121-126 |
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| container_title | Journal of general plant pathology : JGPP |
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| creator | Yakabe, Lani E. Maccree, M. Malendia Sudarshana, Padma McClean, Ali E. Parker, Shane R. Wechter, William P. Presting, Gernot Marutani-Hert, Mizuri Kluepfel, Daniel A. |
| description | Novel PCR primers were developed to amplify a 243-bp fragment of an intergenic region between gene 5 and
tms
2 on the T-DNA of
Agrobacterium tumefaciens
biovar 1
.
These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did not generate extraneous bands, thus facilitating robust real-time PCR detection. |
| doi_str_mv | 10.1007/s10327-012-0364-z |
| format | Article |
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tms
2 on the T-DNA of
Agrobacterium tumefaciens
biovar 1
.
These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did not generate extraneous bands, thus facilitating robust real-time PCR detection.</description><identifier>ISSN: 1345-2630</identifier><identifier>EISSN: 1610-739X</identifier><identifier>DOI: 10.1007/s10327-012-0364-z</identifier><identifier>CODEN: JGPPBQ</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Agriculture ; Bacteria ; Bacterial and Phytoplasma Diseases ; Biomedical and Life Sciences ; Deoxyribonucleic acid ; DNA ; Life Sciences ; Microbiology ; Pathogens ; Plant diseases ; Plant Pathology ; Polymerase chain reaction</subject><ispartof>Journal of general plant pathology : JGPP, 2012-03, Vol.78 (2), p.121-126</ispartof><rights>The Phytopathological Society of Japan and Springer (Outside the USA) 2012</rights><rights>The Phytopathological Society of Japan and Springer 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-e31496822ac1d7dee262e31f0f8ded8ebf4cc07d2bdacb7f5945aa25b728ced63</citedby><cites>FETCH-LOGICAL-c410t-e31496822ac1d7dee262e31f0f8ded8ebf4cc07d2bdacb7f5945aa25b728ced63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10327-012-0364-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10327-012-0364-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Yakabe, Lani E.</creatorcontrib><creatorcontrib>Maccree, M. Malendia</creatorcontrib><creatorcontrib>Sudarshana, Padma</creatorcontrib><creatorcontrib>McClean, Ali E.</creatorcontrib><creatorcontrib>Parker, Shane R.</creatorcontrib><creatorcontrib>Wechter, William P.</creatorcontrib><creatorcontrib>Presting, Gernot</creatorcontrib><creatorcontrib>Marutani-Hert, Mizuri</creatorcontrib><creatorcontrib>Kluepfel, Daniel A.</creatorcontrib><title>Novel PCR primers for detection of genetically diverse virulent Agrobacterium tumefaciens biovar 1 strains</title><title>Journal of general plant pathology : JGPP</title><addtitle>J Gen Plant Pathol</addtitle><description>Novel PCR primers were developed to amplify a 243-bp fragment of an intergenic region between gene 5 and
tms
2 on the T-DNA of
Agrobacterium tumefaciens
biovar 1
.
These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did not generate extraneous bands, thus facilitating robust real-time PCR detection.</description><subject>Agriculture</subject><subject>Bacteria</subject><subject>Bacterial and Phytoplasma Diseases</subject><subject>Biomedical and Life Sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Pathogens</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Polymerase chain reaction</subject><issn>1345-2630</issn><issn>1610-739X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kE1LAzEQhhdRsFZ_gLfgfTUfm_04luIXFBVR8BayyaSkbDc1yS60v96UFTx5mmF43hnmybJrgm8JxtVdIJjRKseE5piVRX44yWakJDivWPN1mnpW8JyWDJ9nFyFsMKaEVXyWbV7cCB16W76jnbdb8AEZ55GGCCpa1yNn0Bp6iFbJrtsjbcfEABqtHzroI1qsvWuliuDtsEVx2IKRykIfUGvdKD0iKEQvbR8uszMjuwBXv3WefT7cfyyf8tXr4_NyscpVQXDMgZGiKWtKpSK60gC0pGlmsKk16BpaUyiFK01bLVVbGd4UXErK24rWCnTJ5tnNtHfn3fcAIYqNG3yfToqGlpxzVrAEkQlS3oXgwYjj-9LvBcHiaFRMRkUyKo5GxSFl6JQJie3X4P8W_x_6AVhcfEg</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Yakabe, Lani E.</creator><creator>Maccree, M. 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tms
2 on the T-DNA of
Agrobacterium tumefaciens
biovar 1
.
These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did not generate extraneous bands, thus facilitating robust real-time PCR detection.</abstract><cop>Japan</cop><pub>Springer Japan</pub><doi>10.1007/s10327-012-0364-z</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of general plant pathology : JGPP, 2012-03, Vol.78 (2), p.121-126 |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Bacteria Bacterial and Phytoplasma Diseases Biomedical and Life Sciences Deoxyribonucleic acid DNA Life Sciences Microbiology Pathogens Plant diseases Plant Pathology Polymerase chain reaction |
| title | Novel PCR primers for detection of genetically diverse virulent Agrobacterium tumefaciens biovar 1 strains |
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