Multiple inserts of gene of interest and selectable marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants
Particle bombardment has been used for soybean transformation for more than 20 yr, but the integration and segregation of transgene inserts in the soybean genome have not been clearly documented. Over the past 5 yr, we processed several hundred transgenic events. In each experiment, the expression c...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2011-05, Vol.47 (2), p.274-281 |
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| container_title | In vitro cellular & developmental biology. Plant |
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| creator | Li, Rugang Bancroft, Bonnie Hutcheon, Carolyn Zhao, Shuiqing Zheng, Suqin Blahut-Beatty, Laureen De Rocher, Jay Simmonds, Daina |
| description | Particle bombardment has been used for soybean transformation for more than 20 yr, but the integration and segregation of transgene inserts in the soybean genome have not been clearly documented. Over the past 5 yr, we processed several hundred transgenic events. In each experiment, the expression cassettes of the gene of interest (GOI) and hygromycin selectable marker gene (SMG) were co-bombarded into soybean at a 1:1 molecular ratio. More than 75% of hygromycin-resistant events also carried the GOI. Molecular analysis of transgenic plants revealed that most events carried multiple inserts of the GOI and the SMG. The GOI and the SMG were linked in selfed T1 and T2 progeny. Segregation analysis of progeny indicated that, in over 98% of the transgenic events, the multiple inserts of the GOI were integrated into the same genetic locus resulting in a 3:1 segregation ratio. Furthermore, the multiple inserts of the GOI are transmitted into succeeding generations, and no recombinants were found. These data indicate that in soybean plants, co-bombarded genes are preferentially integrated and stably segregated as a single genetic locus. |
| doi_str_mv | 10.1007/s11627-011-9359-2 |
| format | Article |
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Over the past 5 yr, we processed several hundred transgenic events. In each experiment, the expression cassettes of the gene of interest (GOI) and hygromycin selectable marker gene (SMG) were co-bombarded into soybean at a 1:1 molecular ratio. More than 75% of hygromycin-resistant events also carried the GOI. Molecular analysis of transgenic plants revealed that most events carried multiple inserts of the GOI and the SMG. The GOI and the SMG were linked in selfed T1 and T2 progeny. Segregation analysis of progeny indicated that, in over 98% of the transgenic events, the multiple inserts of the GOI were integrated into the same genetic locus resulting in a 3:1 segregation ratio. Furthermore, the multiple inserts of the GOI are transmitted into succeeding generations, and no recombinants were found. These data indicate that in soybean plants, co-bombarded genes are preferentially integrated and stably segregated as a single genetic locus.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-011-9359-2</identifier><language>eng</language><publisher>New York: Springer</publisher><subject>Biomedical and Life Sciences ; Cell Biology ; Cell cycle ; Deoxyribonucleic acid ; Developmental Biology ; DNA ; Genes ; Genetic loci ; Genetic testing ; Genetics ; Genomes ; Life Sciences ; Methods ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant Sciences ; Plant Tissue Culture ; Plasmids ; Polymerase chain reaction ; RESEARCH REPORTS ; rev genes ; Rice ; Soybeans ; Transgenes ; Transgenic plants</subject><ispartof>In vitro cellular & developmental biology. Plant, 2011-05, Vol.47 (2), p.274-281</ispartof><rights>2011 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2011</rights><rights>Copyright Society for In Vitro Biology May 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-550c5ef05331d914cde451bb52fc8f99e2dc01cfde072a453c440ff39ffe94443</citedby><cites>FETCH-LOGICAL-c403t-550c5ef05331d914cde451bb52fc8f99e2dc01cfde072a453c440ff39ffe94443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23016747$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23016747$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids></links><search><creatorcontrib>Li, Rugang</creatorcontrib><creatorcontrib>Bancroft, Bonnie</creatorcontrib><creatorcontrib>Hutcheon, Carolyn</creatorcontrib><creatorcontrib>Zhao, Shuiqing</creatorcontrib><creatorcontrib>Zheng, Suqin</creatorcontrib><creatorcontrib>Blahut-Beatty, Laureen</creatorcontrib><creatorcontrib>De Rocher, Jay</creatorcontrib><creatorcontrib>Simmonds, Daina</creatorcontrib><title>Multiple inserts of gene of interest and selectable marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants</title><title>In vitro cellular & developmental biology. Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>Particle bombardment has been used for soybean transformation for more than 20 yr, but the integration and segregation of transgene inserts in the soybean genome have not been clearly documented. Over the past 5 yr, we processed several hundred transgenic events. In each experiment, the expression cassettes of the gene of interest (GOI) and hygromycin selectable marker gene (SMG) were co-bombarded into soybean at a 1:1 molecular ratio. More than 75% of hygromycin-resistant events also carried the GOI. Molecular analysis of transgenic plants revealed that most events carried multiple inserts of the GOI and the SMG. The GOI and the SMG were linked in selfed T1 and T2 progeny. Segregation analysis of progeny indicated that, in over 98% of the transgenic events, the multiple inserts of the GOI were integrated into the same genetic locus resulting in a 3:1 segregation ratio. Furthermore, the multiple inserts of the GOI are transmitted into succeeding generations, and no recombinants were found. These data indicate that in soybean plants, co-bombarded genes are preferentially integrated and stably segregated as a single genetic locus.</description><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell cycle</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental Biology</subject><subject>DNA</subject><subject>Genes</subject><subject>Genetic loci</subject><subject>Genetic testing</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Sciences</subject><subject>Plant Tissue Culture</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>RESEARCH REPORTS</subject><subject>rev genes</subject><subject>Rice</subject><subject>Soybeans</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1OHDEQhEdRIoUQHoADksXdof23Xh8RSkgkEBc4W15PezXL4Fnc3sM-TV41HiZKbpzaan1VpXZ13bmAbwLAXpEQK2k5CMGdMo7LD92J0NZwuVq7j-0NRnOj7epz94VoBwAChD3pft8fxjrsR2RDJiyV2JTYFjPOc8gVC1JlIfeMcMRYw6ahL6E8Y1mwUJDFic_otoSK_QLP4JHVEjK9DPVtTSwwGvK2GczKOkQ2TvFALWYB27btaDpuMGS2H0Ou9LX7lMJIePZ3nnZPP74_3vzkdw-3v26u73jUoCo3BqLBBEYp0TuhY4_aiM3GyBTXyTmUfQQRU49gZdBGRa0hJeVSQqe1Vqfd5eK7L9Prod3sd9Oh5BbpnYS1k2thGyQWKJaJqGDy-zK0zzh6AX6uwS81-FaDn2vwsmnkoqHG5i2W_8bviS4W0Y7qVP6lSAViZbVVfwBeIJfJ</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Li, Rugang</creator><creator>Bancroft, Bonnie</creator><creator>Hutcheon, Carolyn</creator><creator>Zhao, Shuiqing</creator><creator>Zheng, Suqin</creator><creator>Blahut-Beatty, Laureen</creator><creator>De Rocher, Jay</creator><creator>Simmonds, Daina</creator><general>Springer</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20110501</creationdate><title>Multiple inserts of gene of interest and selectable marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants</title><author>Li, Rugang ; Bancroft, Bonnie ; Hutcheon, Carolyn ; Zhao, Shuiqing ; Zheng, Suqin ; Blahut-Beatty, Laureen ; De Rocher, Jay ; Simmonds, Daina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-550c5ef05331d914cde451bb52fc8f99e2dc01cfde072a453c440ff39ffe94443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cell cycle</topic><topic>Deoxyribonucleic acid</topic><topic>Developmental Biology</topic><topic>DNA</topic><topic>Genes</topic><topic>Genetic loci</topic><topic>Genetic testing</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Life Sciences</topic><topic>Methods</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Sciences</topic><topic>Plant Tissue Culture</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>RESEARCH REPORTS</topic><topic>rev genes</topic><topic>Rice</topic><topic>Soybeans</topic><topic>Transgenes</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Rugang</creatorcontrib><creatorcontrib>Bancroft, Bonnie</creatorcontrib><creatorcontrib>Hutcheon, Carolyn</creatorcontrib><creatorcontrib>Zhao, Shuiqing</creatorcontrib><creatorcontrib>Zheng, Suqin</creatorcontrib><creatorcontrib>Blahut-Beatty, Laureen</creatorcontrib><creatorcontrib>De Rocher, Jay</creatorcontrib><creatorcontrib>Simmonds, Daina</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Rugang</au><au>Bancroft, Bonnie</au><au>Hutcheon, Carolyn</au><au>Zhao, Shuiqing</au><au>Zheng, Suqin</au><au>Blahut-Beatty, Laureen</au><au>De Rocher, Jay</au><au>Simmonds, Daina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple inserts of gene of interest and selectable marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2011-05-01</date><risdate>2011</risdate><volume>47</volume><issue>2</issue><spage>274</spage><epage>281</epage><pages>274-281</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>Particle bombardment has been used for soybean transformation for more than 20 yr, but the integration and segregation of transgene inserts in the soybean genome have not been clearly documented. Over the past 5 yr, we processed several hundred transgenic events. In each experiment, the expression cassettes of the gene of interest (GOI) and hygromycin selectable marker gene (SMG) were co-bombarded into soybean at a 1:1 molecular ratio. More than 75% of hygromycin-resistant events also carried the GOI. Molecular analysis of transgenic plants revealed that most events carried multiple inserts of the GOI and the SMG. The GOI and the SMG were linked in selfed T1 and T2 progeny. Segregation analysis of progeny indicated that, in over 98% of the transgenic events, the multiple inserts of the GOI were integrated into the same genetic locus resulting in a 3:1 segregation ratio. Furthermore, the multiple inserts of the GOI are transmitted into succeeding generations, and no recombinants were found. These data indicate that in soybean plants, co-bombarded genes are preferentially integrated and stably segregated as a single genetic locus.</abstract><cop>New York</cop><pub>Springer</pub><doi>10.1007/s11627-011-9359-2</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 1054-5476 |
| ispartof | In vitro cellular & developmental biology. Plant, 2011-05, Vol.47 (2), p.274-281 |
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| source | Springer Nature - Complete Springer Journals; JSTOR |
| subjects | Biomedical and Life Sciences Cell Biology Cell cycle Deoxyribonucleic acid Developmental Biology DNA Genes Genetic loci Genetic testing Genetics Genomes Life Sciences Methods Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Sciences Plant Tissue Culture Plasmids Polymerase chain reaction RESEARCH REPORTS rev genes Rice Soybeans Transgenes Transgenic plants |
| title | Multiple inserts of gene of interest and selectable marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants |
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