Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles
ABSTRACT Purpose Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA. Methods Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing...
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| Veröffentlicht in: | Pharmaceutical research 2012-02, Vol.29 (2), p.362-374 |
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| creator | Kong, Won Ho Bae, Ki Hyun Jo, Sung Duk Kim, Jee Seon Park, Tae Gwan |
| description | ABSTRACT
Purpose
Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA.
Methods
Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines.
Results
Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine.
Conclusions
We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications. |
| doi_str_mv | 10.1007/s11095-011-0554-y |
| format | Article |
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Purpose
Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA.
Methods
Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines.
Results
Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine.
Conclusions
We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1007/s11095-011-0554-y</identifier><identifier>PMID: 21842305</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Cations - chemistry ; Cell Line, Tumor ; Cell Survival ; Cytotoxicity ; General pharmacology ; Gold - chemistry ; Humans ; Lipids ; Lipids - chemistry ; Medical Law ; Medical sciences ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Pharmacology/Toxicology ; Pharmacy ; Polyethyleneimine - chemistry ; Research Paper ; Ribonucleic acid ; RNA ; RNA Interference ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - genetics</subject><ispartof>Pharmaceutical research, 2012-02, Vol.29 (2), p.362-374</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media, LLC 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-174ff61090f80235672e5a1c3cf906e2429bac0d668184fe74e468228437d8cf3</citedby><cites>FETCH-LOGICAL-c466t-174ff61090f80235672e5a1c3cf906e2429bac0d668184fe74e468228437d8cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11095-011-0554-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11095-011-0554-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25557181$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21842305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kong, Won Ho</creatorcontrib><creatorcontrib>Bae, Ki Hyun</creatorcontrib><creatorcontrib>Jo, Sung Duk</creatorcontrib><creatorcontrib>Kim, Jee Seon</creatorcontrib><creatorcontrib>Park, Tae Gwan</creatorcontrib><title>Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><addtitle>Pharm Res</addtitle><description>ABSTRACT
Purpose
Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA.
Methods
Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines.
Results
Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine.
Conclusions
We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications.</description><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Cations - chemistry</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Cytotoxicity</subject><subject>General pharmacology</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Lipids</subject><subject>Lipids - chemistry</subject><subject>Medical Law</subject><subject>Medical sciences</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Polyethyleneimine - chemistry</subject><subject>Research Paper</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>RNA, Small Interfering - genetics</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kEFvFCEYhonR2LX6A7wYYuKR9oOBgTk2Y22bbGrSVOONUAaUZhZWYI3z76Xuak89cfie9_34HoTeUjihAPK0UAqDIEApASE4WZ6hFRWyIwPwb8_RCiTjRElOj9CrUu4BQNGBv0RHjCrOOhArtBlNDSkGi9dhGyYyJlPdhC_SPOFrE9PW5Brs7Ao2BZ97H2xwsWIT2zhFMi411fS7xa9izca6ed7NJuMSbq7P8Ec3h18uL_ir-_G35DV64c1c3JvDe4y-fDq_HS_J-vPF1Xi2Jpb3fSVUcu_7dht4BawTvWROGGo76wfoHeNsuDMWpr5X7RDvJHe8V4wp3slJWd8do_f73m1OP3euVH2fdjm2lXqgshN86ESD6B6yOZWSndfbHDYmL5qCfvCr935186sf_OqlZd4dind3Gzf9T_wT2oAPB8AUa2afTbShPHJCCEkVbRzbc6WN4neXH3_49PY_EAeSgw</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Kong, Won Ho</creator><creator>Bae, Ki Hyun</creator><creator>Jo, Sung Duk</creator><creator>Kim, Jee Seon</creator><creator>Park, Tae Gwan</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20120201</creationdate><title>Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles</title><author>Kong, Won Ho ; Bae, Ki Hyun ; Jo, Sung Duk ; Kim, Jee Seon ; Park, Tae Gwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-174ff61090f80235672e5a1c3cf906e2429bac0d668184fe74e468228437d8cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>Cations - chemistry</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>Cytotoxicity</topic><topic>General pharmacology</topic><topic>Gold - chemistry</topic><topic>Humans</topic><topic>Lipids</topic><topic>Lipids - chemistry</topic><topic>Medical Law</topic><topic>Medical sciences</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - ultrastructure</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Polyethyleneimine - chemistry</topic><topic>Research Paper</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering - administration & dosage</topic><topic>RNA, Small Interfering - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kong, Won Ho</creatorcontrib><creatorcontrib>Bae, Ki Hyun</creatorcontrib><creatorcontrib>Jo, Sung Duk</creatorcontrib><creatorcontrib>Kim, Jee Seon</creatorcontrib><creatorcontrib>Park, Tae Gwan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kong, Won Ho</au><au>Bae, Ki Hyun</au><au>Jo, Sung Duk</au><au>Kim, Jee Seon</au><au>Park, Tae Gwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles</atitle><jtitle>Pharmaceutical research</jtitle><stitle>Pharm Res</stitle><addtitle>Pharm Res</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>29</volume><issue>2</issue><spage>362</spage><epage>374</epage><pages>362-374</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>ABSTRACT
Purpose
Cationic lipid-coated gold nanoparticles were developed for efficient intracellular delivery of therapeutic siRNA.
Methods
Particle formation was characterized by UV-visible spectroscopy, atomic force microscopy, and dynamic light scattering analysis. Cellular uptake, gene silencing effect, and cytotoxicity were investigated in multiple human cancer cell lines.
Results
Nanoparticles had a spherical nanostructure with highly cationic surface charge and could form stable nanosized polyelectrolyte complexes with siRNA via electrostatic interactions; complexes exhibited efficient intracellular uptake and significant gene silencing effect with markedly low cytotoxicity compared to the widely used polycationic carrier, linear polyethyleneimine.
Conclusions
We demonstrated that cationic lipid-coated gold nanoparticles could be widely utilized as efficient and safe siRNA nanocarriers for diverse therapeutic and diagnostic applications.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>21842305</pmid><doi>10.1007/s11095-011-0554-y</doi><tpages>13</tpages></addata></record> |
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| ispartof | Pharmaceutical research, 2012-02, Vol.29 (2), p.362-374 |
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| subjects | Biochemistry Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Cations - chemistry Cell Line, Tumor Cell Survival Cytotoxicity General pharmacology Gold - chemistry Humans Lipids Lipids - chemistry Medical Law Medical sciences Nanoparticles - chemistry Nanoparticles - ultrastructure Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Pharmacology/Toxicology Pharmacy Polyethyleneimine - chemistry Research Paper Ribonucleic acid RNA RNA Interference RNA, Small Interfering - administration & dosage RNA, Small Interfering - genetics |
| title | Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles |
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