Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron
Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental iron added to the sediment. In this study, the effect of...
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| creator | Srinivasa Varadhan, A Khodadoust, Amid P Brenner, Richard C |
| description | Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental iron added to the sediment. In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology for remediation of PCB-contaminated sediments. |
| doi_str_mv | 10.1007/s10295-011-0959-y |
| format | Article |
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In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology for remediation of PCB-contaminated sediments.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-011-0959-y</identifier><identifier>PMID: 21528414</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Bacteria ; Biochemistry ; Biodegradation ; Biodegradation, Environmental ; Bioinformatics ; Biological and medical sciences ; biomarkers ; Biomedical and Life Sciences ; Bioremediation ; Biotechnology ; Chloroflexi - isolation & purification ; Competition ; Contaminated sediments ; Corrosion ; Dehalococcoides ; dehalogenation ; Deltaproteobacteria - isolation & purification ; Electrons ; Environmental Pollutants - metabolism ; Fatty acids ; Fatty Acids - analysis ; freshwater ; Freshwater lakes ; Fundamental and applied biological sciences. Psychology ; Genetic Engineering ; Geobacteraceae ; Geologic Sediments - chemistry ; Geologic Sediments - microbiology ; Hydrogen ; Hydrogen - chemistry ; Inorganic Chemistry ; Iron ; Iron - chemistry ; Iron - metabolism ; Lake sediments ; Lakes ; Life Sciences ; Marine sediments ; methanogens ; Microbial activity ; microbial communities ; Microbiology ; Microorganisms ; Oceans and Seas ; Original Paper ; PCB ; phospholipids ; Phospholipids - chemistry ; Polychlorinated biphenyls ; Polychlorinated Biphenyls - metabolism ; remediation ; Sediment pollution ; sediments ; Studies ; Sulfate reduction ; sulfate-reducing bacteria ; Sulfates ; Toxicity ; Uranium</subject><ispartof>Journal of industrial microbiology & biotechnology, 2011-10, Vol.38 (10), p.1691-1707</ispartof><rights>Society for Industrial Microbiology 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-12a073f41958bdc85baf212e780c19a06ed13f336c7bc8c87318f4923af421473</citedby><cites>FETCH-LOGICAL-c424t-12a073f41958bdc85baf212e780c19a06ed13f336c7bc8c87318f4923af421473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10295-011-0959-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10295-011-0959-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24579611$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21528414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Srinivasa Varadhan, A</creatorcontrib><creatorcontrib>Khodadoust, Amid P</creatorcontrib><creatorcontrib>Brenner, Richard C</creatorcontrib><title>Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><description>Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental iron added to the sediment. In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology for remediation of PCB-contaminated sediments.</description><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Chloroflexi - isolation & purification</subject><subject>Competition</subject><subject>Contaminated sediments</subject><subject>Corrosion</subject><subject>Dehalococcoides</subject><subject>dehalogenation</subject><subject>Deltaproteobacteria - isolation & purification</subject><subject>Electrons</subject><subject>Environmental Pollutants - metabolism</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>freshwater</subject><subject>Freshwater lakes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Engineering</subject><subject>Geobacteraceae</subject><subject>Geologic Sediments - chemistry</subject><subject>Geologic Sediments - microbiology</subject><subject>Hydrogen</subject><subject>Hydrogen - chemistry</subject><subject>Inorganic Chemistry</subject><subject>Iron</subject><subject>Iron - chemistry</subject><subject>Iron - metabolism</subject><subject>Lake sediments</subject><subject>Lakes</subject><subject>Life Sciences</subject><subject>Marine sediments</subject><subject>methanogens</subject><subject>Microbial activity</subject><subject>microbial communities</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Oceans and Seas</subject><subject>Original Paper</subject><subject>PCB</subject><subject>phospholipids</subject><subject>Phospholipids - chemistry</subject><subject>Polychlorinated biphenyls</subject><subject>Polychlorinated Biphenyls - metabolism</subject><subject>remediation</subject><subject>Sediment pollution</subject><subject>sediments</subject><subject>Studies</subject><subject>Sulfate reduction</subject><subject>sulfate-reducing bacteria</subject><subject>Sulfates</subject><subject>Toxicity</subject><subject>Uranium</subject><issn>1367-5435</issn><issn>1476-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kUuPFCEUhStG44yjP8CNEhOXKJdHQS21Mz6SSTTRWROKgm4mXdACFdNbf7l0qsfZmZBA7v3OuXDoupdA3gEh8n0BQgeBCQAmgxjw8VF3CVz2WAgmHrcz6yUWnImL7lkpd4QQISV92l1QEFRx4Jfdn2vvna0oeTSGVGqYl72pIUXUVt05NAeb0xjMHtk0z0sM9YhCRN83H7FNsZo5RFPdhIqbwuxiLU2V07LdoYPLIU3BItPq06l3mnLPod-h7lDIKT7vnnizL-7Feb_qbj9d_9x8wTffPn_dfLjBllNeMVBDJPMcBqHGySoxGk-BOqmIhcGQ3k3APGO9laNVVkkGyvOBMuM5bamwq-7N6nvI6dfiStV3acmxjdRqYJQLLnmDYIXaq0vJzutDDrPJRw1En0LXa-i6ha5Poetj07w6Gy_j7KZ_ivuUG_D2DJhizd5nE20oDxwXcugBGkdXrrRW3Lr8cMP_TX-9irxJ2mxzM779QQnw9t18UEywvxrmpO8</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Srinivasa Varadhan, A</creator><creator>Khodadoust, Amid P</creator><creator>Brenner, Richard C</creator><general>Springer-Verlag</general><general>Springer</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20111001</creationdate><title>Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron</title><author>Srinivasa Varadhan, A ; Khodadoust, Amid P ; Brenner, Richard C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-12a073f41958bdc85baf212e780c19a06ed13f336c7bc8c87318f4923af421473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biodegradation, Environmental</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Bioremediation</topic><topic>Biotechnology</topic><topic>Chloroflexi - isolation & purification</topic><topic>Competition</topic><topic>Contaminated sediments</topic><topic>Corrosion</topic><topic>Dehalococcoides</topic><topic>dehalogenation</topic><topic>Deltaproteobacteria - isolation & purification</topic><topic>Electrons</topic><topic>Environmental Pollutants - metabolism</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>freshwater</topic><topic>Freshwater lakes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Engineering</topic><topic>Geobacteraceae</topic><topic>Geologic Sediments - chemistry</topic><topic>Geologic Sediments - microbiology</topic><topic>Hydrogen</topic><topic>Hydrogen - chemistry</topic><topic>Inorganic Chemistry</topic><topic>Iron</topic><topic>Iron - chemistry</topic><topic>Iron - metabolism</topic><topic>Lake sediments</topic><topic>Lakes</topic><topic>Life Sciences</topic><topic>Marine sediments</topic><topic>methanogens</topic><topic>Microbial activity</topic><topic>microbial communities</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Oceans and Seas</topic><topic>Original Paper</topic><topic>PCB</topic><topic>phospholipids</topic><topic>Phospholipids - chemistry</topic><topic>Polychlorinated biphenyls</topic><topic>Polychlorinated Biphenyls - metabolism</topic><topic>remediation</topic><topic>Sediment pollution</topic><topic>sediments</topic><topic>Studies</topic><topic>Sulfate 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Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of industrial microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Srinivasa Varadhan, A</au><au>Khodadoust, Amid P</au><au>Brenner, Richard C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>38</volume><issue>10</issue><spage>1691</spage><epage>1707</epage><pages>1691-1707</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental iron added to the sediment. In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology for remediation of PCB-contaminated sediments.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21528414</pmid><doi>10.1007/s10295-011-0959-y</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1367-5435 |
| ispartof | Journal of industrial microbiology & biotechnology, 2011-10, Vol.38 (10), p.1691-1707 |
| issn | 1367-5435 1476-5535 |
| language | eng |
| recordid | cdi_proquest_journals_893245474 |
| source | MEDLINE; Access via Oxford University Press (Open Access Collection); SpringerNature Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Bacteria Biochemistry Biodegradation Biodegradation, Environmental Bioinformatics Biological and medical sciences biomarkers Biomedical and Life Sciences Bioremediation Biotechnology Chloroflexi - isolation & purification Competition Contaminated sediments Corrosion Dehalococcoides dehalogenation Deltaproteobacteria - isolation & purification Electrons Environmental Pollutants - metabolism Fatty acids Fatty Acids - analysis freshwater Freshwater lakes Fundamental and applied biological sciences. Psychology Genetic Engineering Geobacteraceae Geologic Sediments - chemistry Geologic Sediments - microbiology Hydrogen Hydrogen - chemistry Inorganic Chemistry Iron Iron - chemistry Iron - metabolism Lake sediments Lakes Life Sciences Marine sediments methanogens Microbial activity microbial communities Microbiology Microorganisms Oceans and Seas Original Paper PCB phospholipids Phospholipids - chemistry Polychlorinated biphenyls Polychlorinated Biphenyls - metabolism remediation Sediment pollution sediments Studies Sulfate reduction sulfate-reducing bacteria Sulfates Toxicity Uranium |
| title | Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T16%3A11%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20biostimulation%20on%20the%20microbial%20community%20in%20PCB-contaminated%20sediments%20through%20periodic%20amendment%20of%20sediment%20with%20iron&rft.jtitle=Journal%20of%20industrial%20microbiology%20&%20biotechnology&rft.au=Srinivasa%20Varadhan,%20A&rft.date=2011-10-01&rft.volume=38&rft.issue=10&rft.spage=1691&rft.epage=1707&rft.pages=1691-1707&rft.issn=1367-5435&rft.eissn=1476-5535&rft_id=info:doi/10.1007/s10295-011-0959-y&rft_dat=%3Cproquest_cross%3E2464723921%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=893245474&rft_id=info:pmid/21528414&rfr_iscdi=true |