Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron

Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental iron added to the sediment. In this study, the effect of...

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Veröffentlicht in:Journal of industrial microbiology & biotechnology 2011-10, Vol.38 (10), p.1691-1707
Hauptverfasser: Srinivasa Varadhan, A, Khodadoust, Amid P, Brenner, Richard C
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Khodadoust, Amid P
Brenner, Richard C
description Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental iron added to the sediment. In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology for remediation of PCB-contaminated sediments.
doi_str_mv 10.1007/s10295-011-0959-y
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In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology for remediation of PCB-contaminated sediments.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-011-0959-y</identifier><identifier>PMID: 21528414</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Bacteria ; Biochemistry ; Biodegradation ; Biodegradation, Environmental ; Bioinformatics ; Biological and medical sciences ; biomarkers ; Biomedical and Life Sciences ; Bioremediation ; Biotechnology ; Chloroflexi - isolation &amp; purification ; Competition ; Contaminated sediments ; Corrosion ; Dehalococcoides ; dehalogenation ; Deltaproteobacteria - isolation &amp; purification ; Electrons ; Environmental Pollutants - metabolism ; Fatty acids ; Fatty Acids - analysis ; freshwater ; Freshwater lakes ; Fundamental and applied biological sciences. 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In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. 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Khodadoust, Amid P ; Brenner, Richard C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-12a073f41958bdc85baf212e780c19a06ed13f336c7bc8c87318f4923af421473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biodegradation, Environmental</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Bioremediation</topic><topic>Biotechnology</topic><topic>Chloroflexi - isolation &amp; purification</topic><topic>Competition</topic><topic>Contaminated sediments</topic><topic>Corrosion</topic><topic>Dehalococcoides</topic><topic>dehalogenation</topic><topic>Deltaproteobacteria - isolation &amp; purification</topic><topic>Electrons</topic><topic>Environmental Pollutants - metabolism</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>freshwater</topic><topic>Freshwater lakes</topic><topic>Fundamental and applied biological sciences. 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biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Srinivasa Varadhan, A</au><au>Khodadoust, Amid P</au><au>Brenner, Richard C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron</atitle><jtitle>Journal of industrial microbiology &amp; biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>38</volume><issue>10</issue><spage>1691</spage><epage>1707</epage><pages>1691-1707</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>Reductive dehalogenation of polychlorinated biphenyls (PCBs) by indigenous dehalorespiring microorganisms in contaminated sediments may be enhanced via biostimulation by supplying hydrogen generated through the anaerobic corrosion of elemental iron added to the sediment. In this study, the effect of periodic amendment of sediment with various dosages of iron on the microbial community present in sediment was investigated using phospholipid fatty acid analysis (PLFA) over a period of 18 months. Three PCB-contaminated sediments (two freshwater lake sediments and one marine sediment) were used. Signature biomarker analysis of the microbial community present in all three sediments revealed the enrichment of Dehalococcoides species, the population of which was sustained for a longer period of time when the sediment microcosms were amended with the lower dosage of iron (0.01 g iron per g dry sediment) every 6 months as compared to the blank system (without iron). Lower microbial stress levels were reported for the system periodically amended with 0.01 g of iron per g dry sediment every 6 months, thus reducing the competition from other hydrogen-utilizing microorganisms like methanogens, iron reducers, and sulfate reducers. The concentration of hydrogen in the system was found to be an important factor influencing the shift in microbial communities in all sediments with time. Periodic amendment of sediment with larger dosages of iron every 3 months resulted in the early prevalence of Geobacteraceae and sulfate-reducing bacteria followed by methanogens. An average pH of 8.4 (range of 8.2–8.6) and an average hydrogen concentration of 0.75% (range of 0.3–1.2%) observed between 6 and 15 months of the study were found to be conducive to sustaining the population of Dehalococcoides species in the three sediments amended with 0.01 g iron per g dry sediment. Biostimulation of indigenous PCB dechlorinators by the periodic amendment of contaminated sediments with low dosages of iron metal may therefore be an effective technology for remediation of PCB-contaminated sediments.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21528414</pmid><doi>10.1007/s10295-011-0959-y</doi><tpages>17</tpages></addata></record>
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source MEDLINE; Access via Oxford University Press (Open Access Collection); SpringerNature Journals; Oxford University Press Journals All Titles (1996-Current)
subjects Bacteria
Biochemistry
Biodegradation
Biodegradation, Environmental
Bioinformatics
Biological and medical sciences
biomarkers
Biomedical and Life Sciences
Bioremediation
Biotechnology
Chloroflexi - isolation & purification
Competition
Contaminated sediments
Corrosion
Dehalococcoides
dehalogenation
Deltaproteobacteria - isolation & purification
Electrons
Environmental Pollutants - metabolism
Fatty acids
Fatty Acids - analysis
freshwater
Freshwater lakes
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Geobacteraceae
Geologic Sediments - chemistry
Geologic Sediments - microbiology
Hydrogen
Hydrogen - chemistry
Inorganic Chemistry
Iron
Iron - chemistry
Iron - metabolism
Lake sediments
Lakes
Life Sciences
Marine sediments
methanogens
Microbial activity
microbial communities
Microbiology
Microorganisms
Oceans and Seas
Original Paper
PCB
phospholipids
Phospholipids - chemistry
Polychlorinated biphenyls
Polychlorinated Biphenyls - metabolism
remediation
Sediment pollution
sediments
Studies
Sulfate reduction
sulfate-reducing bacteria
Sulfates
Toxicity
Uranium
title Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron
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