Kinetic characterisation of recombinant Corynebacterium glutamicum NAD^sup +^-dependent LDH over-expressed in E. coli and its rescue of an lldD ^sup -^ phenotype in C. glutamicum: the issue of reversibility re-examined

The ldh gene of Corynebacterium glutamicum ATCC 13032 (gene symbol cg3219, encoding a 314 residue NAD^sup +^-dependent l-(+)-lactate dehydrogenase, EC 1.1.1.27) was cloned into the expression vector pKK388-1 and over-expressed in an ldhA-null E. coli TG1 strain upon isopropyl-β-D-thiogalactopyranosi...

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Veröffentlicht in:	Archives of microbiology 2011-10, Vol.193 (10), p.731
Hauptverfasser:	Sharkey, Michael A, Maher, Marcus A, Guyonvarch, Armel, Engel, Paul C
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbon Carbon sources Dehydrogenases E coli Enzyme kinetics Equilibrium Oxidation Plasmids
Online-Zugang:	Volltext
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