Percentage of LFA-1^sup +^ and ICAM-1^sup +^ peripheral blood mononuclear cells in children and adolescents with Type 1 diabetes does not distinguish patients with vascular complications

Percentage of LFA-1^sup +^ and ICAM-1^sup +^ peripheral blood mononuclear cells in children and adolescents with Type 1 diabetes does not distinguish patients with vascular complications

There are only few studies evaluating lymphocytes activation in the diabetic vascular complications. ICAM-1/LFA-1 adhesion molecules not only participate in the lymphocyte T proliferation but also mediate leukocyte migration to the site of inflammation. We assess a relationship between the percentag...

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Veröffentlicht in:Folia histochemica et cytobiologica 2009-03, Vol.47 (2), p.243
Hauptverfasser: Pawlowski, Przemyslaw, Urban, Miroslawa, Stasiak-Barmuta, Anna, Myoeliwiec, Janusz, Pawlowska, Malgorzata
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container_title Folia histochemica et cytobiologica
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creator Pawlowski, Przemyslaw
Urban, Miroslawa
Stasiak-Barmuta, Anna
Myoeliwiec, Janusz
Pawlowska, Malgorzata
description There are only few studies evaluating lymphocytes activation in the diabetic vascular complications. ICAM-1/LFA-1 adhesion molecules not only participate in the lymphocyte T proliferation but also mediate leukocyte migration to the site of inflammation. We assess a relationship between the percentage of ICAM-1 and LFA-1 expressing PBMCs and the evolution of vascular complications in T1D in children and adolescents. The study was carried out on 60 children and adolescents with T1D (aged 9-20): (a) T1D lasting 5 years (n=20), without complications c) T1D lasting >5 years complicated with microalbuminuria, arterial hypertension, diabetic retinopathy (20 n). 20 healthy volunteers, age and sex matched constituted the control group. The expression of adhesion molecules was evaluated by using three-color flow cytometry. In children and adolescents with T1D
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ICAM-1/LFA-1 adhesion molecules not only participate in the lymphocyte T proliferation but also mediate leukocyte migration to the site of inflammation. We assess a relationship between the percentage of ICAM-1 and LFA-1 expressing PBMCs and the evolution of vascular complications in T1D in children and adolescents. The study was carried out on 60 children and adolescents with T1D (aged 9-20): (a) T1D lasting &lt;5 years (n=20), (b) T1D lasting &gt;5 years (n=20), without complications c) T1D lasting &gt;5 years complicated with microalbuminuria, arterial hypertension, diabetic retinopathy (20 n). 20 healthy volunteers, age and sex matched constituted the control group. The expression of adhesion molecules was evaluated by using three-color flow cytometry. In children and adolescents with T1D &lt;5 years, the percentage of ICAM-1+ and LFA-1+ PBMCs was decreased vs. controls (p&lt;0.05 and p&lt;0.001, respectively). Both in patients with T1D&gt;5 years without vascular complications and in T1D with vascular disease the percentage of LFA-1+ T lymphocytes was significantly reduced in the peripheral blood (p&lt;0.001 vs. healthy controls). In conclusion the percentage of LFA-1+ and ICAM-1+ PBMCs does not distinguish patients with vascular complications however decreased percentage of LFA-1+ PMBCs could serve as a nonspecific marker of the development of local inflammatory process in Type 1 diabetes.</description><identifier>ISSN: 0239-8508</identifier><identifier>EISSN: 1897-5631</identifier><language>eng</language><publisher>Bialystok: Wydawnictwo Via Medica</publisher><ispartof>Folia histochemica et cytobiologica, 2009-03, Vol.47 (2), p.243</ispartof><rights>Copyright Folia Histochemica et Cytobiologica 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Pawlowski, Przemyslaw</creatorcontrib><creatorcontrib>Urban, Miroslawa</creatorcontrib><creatorcontrib>Stasiak-Barmuta, Anna</creatorcontrib><creatorcontrib>Myoeliwiec, Janusz</creatorcontrib><creatorcontrib>Pawlowska, Malgorzata</creatorcontrib><title>Percentage of LFA-1^sup +^ and ICAM-1^sup +^ peripheral blood mononuclear cells in children and adolescents with Type 1 diabetes does not distinguish patients with vascular complications</title><title>Folia histochemica et cytobiologica</title><description>There are only few studies evaluating lymphocytes activation in the diabetic vascular complications. 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Both in patients with T1D&gt;5 years without vascular complications and in T1D with vascular disease the percentage of LFA-1+ T lymphocytes was significantly reduced in the peripheral blood (p&lt;0.001 vs. healthy controls). 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ICAM-1/LFA-1 adhesion molecules not only participate in the lymphocyte T proliferation but also mediate leukocyte migration to the site of inflammation. We assess a relationship between the percentage of ICAM-1 and LFA-1 expressing PBMCs and the evolution of vascular complications in T1D in children and adolescents. The study was carried out on 60 children and adolescents with T1D (aged 9-20): (a) T1D lasting &lt;5 years (n=20), (b) T1D lasting &gt;5 years (n=20), without complications c) T1D lasting &gt;5 years complicated with microalbuminuria, arterial hypertension, diabetic retinopathy (20 n). 20 healthy volunteers, age and sex matched constituted the control group. The expression of adhesion molecules was evaluated by using three-color flow cytometry. In children and adolescents with T1D &lt;5 years, the percentage of ICAM-1+ and LFA-1+ PBMCs was decreased vs. controls (p&lt;0.05 and p&lt;0.001, respectively). 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title Percentage of LFA-1^sup +^ and ICAM-1^sup +^ peripheral blood mononuclear cells in children and adolescents with Type 1 diabetes does not distinguish patients with vascular complications
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