Cold-Induced Biochemical and Molecular Changes in Alfalfa Populations Selectively Improved for Freezing Tolerance
Synthetic populations obtained after successive cycles of recurrent selection for superior tolerance to freezing were used to probe the molecular bases of superior adaptation to cold in alfalfa (Medicago sativa L.). The initial genetic backgrounds and populations derived from the cultivars Apica and...
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| Veröffentlicht in: | Crop science 2011-09, Vol.51 (5), p.2132-2144 |
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| creator | Castonguay, Yves Bertrand, Annick Michaud, Real Laberge, Serge |
| description | Synthetic populations obtained after successive cycles of recurrent selection for superior tolerance to freezing were used to probe the molecular bases of superior adaptation to cold in alfalfa (Medicago sativa L.). The initial genetic backgrounds and populations derived from the cultivars Apica and Evolution were assessed for their freezing tolerance and cold-induced molecular changes using plants acclimated to natural hardening conditions in an unheated greenhouse. Significant increase in freezing tolerance in response to recurrent selection was confirmed by the determination of the lethal temperature for 50% of the plants (LT50). Improvement of freezing tolerance was associated with differences in cold-induced molecular changes. Starch reserves in crowns progressively declined during fall hardening with a more extensive mobilization observed in advanced cycles of selection. Concentrations of cryoprotective sugars and of the amino acids proline, asparagine, and arginine increased during fall hardening and were significantly higher in recurrently selected populations than in the initial backgrounds. Transcript levels of two cold-regulated (COR) genes, cas15 and GaS, were strongly up-regulated during fall hardening and significantly increased in response to selection mostly in the Apica background. Our results provide evidence that recurrent selection for superior freezing tolerance in alfalfa induces marked changes in traits associated with the cold acclimation process |
| doi_str_mv | 10.2135/cropsci2011.02.0060 |
| format | Article |
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The initial genetic backgrounds and populations derived from the cultivars Apica and Evolution were assessed for their freezing tolerance and cold-induced molecular changes using plants acclimated to natural hardening conditions in an unheated greenhouse. Significant increase in freezing tolerance in response to recurrent selection was confirmed by the determination of the lethal temperature for 50% of the plants (LT50). Improvement of freezing tolerance was associated with differences in cold-induced molecular changes. Starch reserves in crowns progressively declined during fall hardening with a more extensive mobilization observed in advanced cycles of selection. Concentrations of cryoprotective sugars and of the amino acids proline, asparagine, and arginine increased during fall hardening and were significantly higher in recurrently selected populations than in the initial backgrounds. Transcript levels of two cold-regulated (COR) genes, cas15 and GaS, were strongly up-regulated during fall hardening and significantly increased in response to selection mostly in the Apica background. Our results provide evidence that recurrent selection for superior freezing tolerance in alfalfa induces marked changes in traits associated with the cold acclimation process</description><identifier>ISSN: 1435-0653</identifier><identifier>ISSN: 0011-183X</identifier><identifier>EISSN: 1435-0653</identifier><identifier>DOI: 10.2135/cropsci2011.02.0060</identifier><identifier>CODEN: CRPSAY</identifier><language>eng</language><publisher>Madison, WI: Crop Science Society of America</publisher><subject>acclimation ; Acclimatization ; Adaptation to environment and cultivation conditions ; Agronomy. Soil science and plant productions ; Alfalfa ; Amino acids ; arginine ; asparagine ; Biological and medical sciences ; Cold ; cold tolerance ; Cultivars ; evolution ; Fertilizers ; Freezing ; Fundamental and applied biological sciences. Psychology ; Gene expression ; gene expression regulation ; genes ; Genetic diversity ; Genetics and breeding of economic plants ; Genomes ; greenhouses ; Medicago sativa ; Mosses ; proline ; recurrent selection ; selection response ; starch ; synthetic populations ; Temperature ; Varietal selection. 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The initial genetic backgrounds and populations derived from the cultivars Apica and Evolution were assessed for their freezing tolerance and cold-induced molecular changes using plants acclimated to natural hardening conditions in an unheated greenhouse. Significant increase in freezing tolerance in response to recurrent selection was confirmed by the determination of the lethal temperature for 50% of the plants (LT50). Improvement of freezing tolerance was associated with differences in cold-induced molecular changes. Starch reserves in crowns progressively declined during fall hardening with a more extensive mobilization observed in advanced cycles of selection. Concentrations of cryoprotective sugars and of the amino acids proline, asparagine, and arginine increased during fall hardening and were significantly higher in recurrently selected populations than in the initial backgrounds. Transcript levels of two cold-regulated (COR) genes, cas15 and GaS, were strongly up-regulated during fall hardening and significantly increased in response to selection mostly in the Apica background. Our results provide evidence that recurrent selection for superior freezing tolerance in alfalfa induces marked changes in traits associated with the cold acclimation process</description><subject>acclimation</subject><subject>Acclimatization</subject><subject>Adaptation to environment and cultivation conditions</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Alfalfa</subject><subject>Amino acids</subject><subject>arginine</subject><subject>asparagine</subject><subject>Biological and medical sciences</subject><subject>Cold</subject><subject>cold tolerance</subject><subject>Cultivars</subject><subject>evolution</subject><subject>Fertilizers</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>Genetic diversity</subject><subject>Genetics and breeding of economic plants</subject><subject>Genomes</subject><subject>greenhouses</subject><subject>Medicago sativa</subject><subject>Mosses</subject><subject>proline</subject><subject>recurrent selection</subject><subject>selection response</subject><subject>starch</subject><subject>synthetic populations</subject><subject>Temperature</subject><subject>Varietal selection. 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Soil science and plant productions</topic><topic>Alfalfa</topic><topic>Amino acids</topic><topic>arginine</topic><topic>asparagine</topic><topic>Biological and medical sciences</topic><topic>Cold</topic><topic>cold tolerance</topic><topic>Cultivars</topic><topic>evolution</topic><topic>Fertilizers</topic><topic>Freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>genes</topic><topic>Genetic diversity</topic><topic>Genetics and breeding of economic plants</topic><topic>Genomes</topic><topic>greenhouses</topic><topic>Medicago sativa</topic><topic>Mosses</topic><topic>proline</topic><topic>recurrent selection</topic><topic>selection response</topic><topic>starch</topic><topic>synthetic populations</topic><topic>Temperature</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castonguay, Yves</creatorcontrib><creatorcontrib>Bertrand, Annick</creatorcontrib><creatorcontrib>Michaud, Real</creatorcontrib><creatorcontrib>Laberge, Serge</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>SIRS Editorial</collection><jtitle>Crop science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castonguay, Yves</au><au>Bertrand, Annick</au><au>Michaud, Real</au><au>Laberge, Serge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cold-Induced Biochemical and Molecular Changes in Alfalfa Populations Selectively Improved for Freezing Tolerance</atitle><jtitle>Crop science</jtitle><date>2011-09</date><risdate>2011</risdate><volume>51</volume><issue>5</issue><spage>2132</spage><epage>2144</epage><pages>2132-2144</pages><issn>1435-0653</issn><issn>0011-183X</issn><eissn>1435-0653</eissn><coden>CRPSAY</coden><abstract>Synthetic populations obtained after successive cycles of recurrent selection for superior tolerance to freezing were used to probe the molecular bases of superior adaptation to cold in alfalfa (Medicago sativa L.). The initial genetic backgrounds and populations derived from the cultivars Apica and Evolution were assessed for their freezing tolerance and cold-induced molecular changes using plants acclimated to natural hardening conditions in an unheated greenhouse. Significant increase in freezing tolerance in response to recurrent selection was confirmed by the determination of the lethal temperature for 50% of the plants (LT50). Improvement of freezing tolerance was associated with differences in cold-induced molecular changes. Starch reserves in crowns progressively declined during fall hardening with a more extensive mobilization observed in advanced cycles of selection. Concentrations of cryoprotective sugars and of the amino acids proline, asparagine, and arginine increased during fall hardening and were significantly higher in recurrently selected populations than in the initial backgrounds. Transcript levels of two cold-regulated (COR) genes, cas15 and GaS, were strongly up-regulated during fall hardening and significantly increased in response to selection mostly in the Apica background. Our results provide evidence that recurrent selection for superior freezing tolerance in alfalfa induces marked changes in traits associated with the cold acclimation process</abstract><cop>Madison, WI</cop><pub>Crop Science Society of America</pub><doi>10.2135/cropsci2011.02.0060</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | acclimation Acclimatization Adaptation to environment and cultivation conditions Agronomy. Soil science and plant productions Alfalfa Amino acids arginine asparagine Biological and medical sciences Cold cold tolerance Cultivars evolution Fertilizers Freezing Fundamental and applied biological sciences. Psychology Gene expression gene expression regulation genes Genetic diversity Genetics and breeding of economic plants Genomes greenhouses Medicago sativa Mosses proline recurrent selection selection response starch synthetic populations Temperature Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Cold-Induced Biochemical and Molecular Changes in Alfalfa Populations Selectively Improved for Freezing Tolerance |
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