Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis
Riboflavin mediates many bioprocesses associated with the generation of hydrogen peroxide (H 2 O 2 ), a cellular signal that regulates defense responses in plants. Although plants can synthesize riboflavin, the levels vary widely in different organs and during different stages of development, indica...
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| Veröffentlicht in: | Plant molecular biology 2011-09, Vol.77 (1-2), p.185-201 |
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| creator | Deng, Benliang Deng, Sheng Sun, Feng Zhang, Shujian Dong, Hansong |
| description | Riboflavin mediates many bioprocesses associated with the generation of hydrogen peroxide (H
2
O
2
), a cellular signal that regulates defense responses in plants. Although plants can synthesize riboflavin, the levels vary widely in different organs and during different stages of development, indicating that changes in riboflavin levels may have physiological effects. Here, we show that changing riboflavin content affects H
2
O
2
accumulation and a pathogen defense in
Arabidopsis thaliana
. Leaf content of free riboflavin was modulated by ectopic expression of the turtle gene encoding riboflavin-binding protein (RfBP). The
RfBP
-expressing
Arabidopsis thaliana
(REAT) plants produced the RfBP protein that possessed riboflavin-binding activity. Compared with the wild-type plant, several tested REAT lines had >70% less flavins of free form. This change accompanied an elevation in the level of H
2
O
2
and an enhancement of plant resistance to a bacterial pathogen. All the observed REAT characters were eliminated due to
RfBP
silencing (RfBPi) under REAT background. When an H
2
O
2
scavenger was applied, H
2
O
2
level declined in all the plants, and REAT no longer exhibited the phenotype of resistance enhancement. However, treatment with an NADPH oxidase inhibitor diminished H
2
O
2
content and pathogen defense in wild-type and RfBPi but not in REAT. Our results suggest that the intrinsic down-regulation of free flavins is responsible for NADPH oxidase-independent H
2
O
2
accumulation and the pathogen defense. |
| doi_str_mv | 10.1007/s11103-011-9802-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_884714773</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2432362201</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-3737698a6b981c04a64a22e4897d4951e4f46dfc07cb15a81f6bd5c53d9fb4be3</originalsourceid><addsrcrecordid>eNp1kMtOwzAQRS0EouXxAWyQxd7giZ04WVblKSGxgbXlxOOSqtjBToD-PYEWWLEaaebcO9Ih5AT4OXCuLhIAcME4AKtKnjG-Q6aQK8FynpW7ZMqhUExKyCbkIKUl52NKFPtkkoHKeAXVlCwvw7tnERfDyvRt8DQ46iIijW0d3Mq8tZ42wffoe9p6OzSY6PPaxrBATzuM4aO1SI231NDO9M_fe4sOfcIxQGfR1K0NXWrTEdlzZpXweDsPydP11eP8lt0_3NzNZ_eskaLomVBCFVVpiroqoeHSFNJkGcqyUlZWOaB0srCu4aqpITcluKK2eZMLW7la1igOydmmt4vhdcDU62UYoh9f6rKUCqRSYoRgAzUxpBTR6S62LyauNXD9JVdv5OpRrv6Sq_mYOd0WD_UL2t_Ej80RyDZAGk9-gfHv8_-tn0kXhY0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>884714773</pqid></control><display><type>article</type><title>Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Deng, Benliang ; Deng, Sheng ; Sun, Feng ; Zhang, Shujian ; Dong, Hansong</creator><creatorcontrib>Deng, Benliang ; Deng, Sheng ; Sun, Feng ; Zhang, Shujian ; Dong, Hansong</creatorcontrib><description>Riboflavin mediates many bioprocesses associated with the generation of hydrogen peroxide (H
2
O
2
), a cellular signal that regulates defense responses in plants. Although plants can synthesize riboflavin, the levels vary widely in different organs and during different stages of development, indicating that changes in riboflavin levels may have physiological effects. Here, we show that changing riboflavin content affects H
2
O
2
accumulation and a pathogen defense in
Arabidopsis thaliana
. Leaf content of free riboflavin was modulated by ectopic expression of the turtle gene encoding riboflavin-binding protein (RfBP). The
RfBP
-expressing
Arabidopsis thaliana
(REAT) plants produced the RfBP protein that possessed riboflavin-binding activity. Compared with the wild-type plant, several tested REAT lines had >70% less flavins of free form. This change accompanied an elevation in the level of H
2
O
2
and an enhancement of plant resistance to a bacterial pathogen. All the observed REAT characters were eliminated due to
RfBP
silencing (RfBPi) under REAT background. When an H
2
O
2
scavenger was applied, H
2
O
2
level declined in all the plants, and REAT no longer exhibited the phenotype of resistance enhancement. However, treatment with an NADPH oxidase inhibitor diminished H
2
O
2
content and pathogen defense in wild-type and RfBPi but not in REAT. Our results suggest that the intrinsic down-regulation of free flavins is responsible for NADPH oxidase-independent H
2
O
2
accumulation and the pathogen defense.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/s11103-011-9802-0</identifier><identifier>PMID: 21720919</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis - microbiology ; Arabidopsis Proteins - metabolism ; Biochemistry ; Biomedical and Life Sciences ; Developmental stages ; Down-Regulation ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Immunity, Innate ; Life Sciences ; NADPH Oxidases - metabolism ; Pathogens ; Phenotype ; Plant biology ; Plant Pathology ; Plant resistance ; Plant Sciences ; Plants, Genetically Modified - metabolism ; Plants, Genetically Modified - microbiology ; Proteins ; Riboflavin - genetics ; Riboflavin - metabolism ; Turtles - genetics</subject><ispartof>Plant molecular biology, 2011-09, Vol.77 (1-2), p.185-201</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-3737698a6b981c04a64a22e4897d4951e4f46dfc07cb15a81f6bd5c53d9fb4be3</citedby><cites>FETCH-LOGICAL-c436t-3737698a6b981c04a64a22e4897d4951e4f46dfc07cb15a81f6bd5c53d9fb4be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11103-011-9802-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11103-011-9802-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21720919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Benliang</creatorcontrib><creatorcontrib>Deng, Sheng</creatorcontrib><creatorcontrib>Sun, Feng</creatorcontrib><creatorcontrib>Zhang, Shujian</creatorcontrib><creatorcontrib>Dong, Hansong</creatorcontrib><title>Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><addtitle>Plant Mol Biol</addtitle><description>Riboflavin mediates many bioprocesses associated with the generation of hydrogen peroxide (H
2
O
2
), a cellular signal that regulates defense responses in plants. Although plants can synthesize riboflavin, the levels vary widely in different organs and during different stages of development, indicating that changes in riboflavin levels may have physiological effects. Here, we show that changing riboflavin content affects H
2
O
2
accumulation and a pathogen defense in
Arabidopsis thaliana
. Leaf content of free riboflavin was modulated by ectopic expression of the turtle gene encoding riboflavin-binding protein (RfBP). The
RfBP
-expressing
Arabidopsis thaliana
(REAT) plants produced the RfBP protein that possessed riboflavin-binding activity. Compared with the wild-type plant, several tested REAT lines had >70% less flavins of free form. This change accompanied an elevation in the level of H
2
O
2
and an enhancement of plant resistance to a bacterial pathogen. All the observed REAT characters were eliminated due to
RfBP
silencing (RfBPi) under REAT background. When an H
2
O
2
scavenger was applied, H
2
O
2
level declined in all the plants, and REAT no longer exhibited the phenotype of resistance enhancement. However, treatment with an NADPH oxidase inhibitor diminished H
2
O
2
content and pathogen defense in wild-type and RfBPi but not in REAT. Our results suggest that the intrinsic down-regulation of free flavins is responsible for NADPH oxidase-independent H
2
O
2
accumulation and the pathogen defense.</description><subject>Animals</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Developmental stages</subject><subject>Down-Regulation</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Immunity, Innate</subject><subject>Life Sciences</subject><subject>NADPH Oxidases - metabolism</subject><subject>Pathogens</subject><subject>Phenotype</subject><subject>Plant biology</subject><subject>Plant Pathology</subject><subject>Plant resistance</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>Proteins</subject><subject>Riboflavin - genetics</subject><subject>Riboflavin - metabolism</subject><subject>Turtles - genetics</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kMtOwzAQRS0EouXxAWyQxd7giZ04WVblKSGxgbXlxOOSqtjBToD-PYEWWLEaaebcO9Ih5AT4OXCuLhIAcME4AKtKnjG-Q6aQK8FynpW7ZMqhUExKyCbkIKUl52NKFPtkkoHKeAXVlCwvw7tnERfDyvRt8DQ46iIijW0d3Mq8tZ42wffoe9p6OzSY6PPaxrBATzuM4aO1SI231NDO9M_fe4sOfcIxQGfR1K0NXWrTEdlzZpXweDsPydP11eP8lt0_3NzNZ_eskaLomVBCFVVpiroqoeHSFNJkGcqyUlZWOaB0srCu4aqpITcluKK2eZMLW7la1igOydmmt4vhdcDU62UYoh9f6rKUCqRSYoRgAzUxpBTR6S62LyauNXD9JVdv5OpRrv6Sq_mYOd0WD_UL2t_Ej80RyDZAGk9-gfHv8_-tn0kXhY0</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Deng, Benliang</creator><creator>Deng, Sheng</creator><creator>Sun, Feng</creator><creator>Zhang, Shujian</creator><creator>Dong, Hansong</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20110901</creationdate><title>Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis</title><author>Deng, Benliang ; Deng, Sheng ; Sun, Feng ; Zhang, Shujian ; Dong, Hansong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-3737698a6b981c04a64a22e4897d4951e4f46dfc07cb15a81f6bd5c53d9fb4be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Developmental stages</topic><topic>Down-Regulation</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Immunity, Innate</topic><topic>Life Sciences</topic><topic>NADPH Oxidases - metabolism</topic><topic>Pathogens</topic><topic>Phenotype</topic><topic>Plant biology</topic><topic>Plant Pathology</topic><topic>Plant resistance</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plants, Genetically Modified - microbiology</topic><topic>Proteins</topic><topic>Riboflavin - genetics</topic><topic>Riboflavin - metabolism</topic><topic>Turtles - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Benliang</creatorcontrib><creatorcontrib>Deng, Sheng</creatorcontrib><creatorcontrib>Sun, Feng</creatorcontrib><creatorcontrib>Zhang, Shujian</creatorcontrib><creatorcontrib>Dong, Hansong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Benliang</au><au>Deng, Sheng</au><au>Sun, Feng</au><au>Zhang, Shujian</au><au>Dong, Hansong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis</atitle><jtitle>Plant molecular biology</jtitle><stitle>Plant Mol Biol</stitle><addtitle>Plant Mol Biol</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>77</volume><issue>1-2</issue><spage>185</spage><epage>201</epage><pages>185-201</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Riboflavin mediates many bioprocesses associated with the generation of hydrogen peroxide (H
2
O
2
), a cellular signal that regulates defense responses in plants. Although plants can synthesize riboflavin, the levels vary widely in different organs and during different stages of development, indicating that changes in riboflavin levels may have physiological effects. Here, we show that changing riboflavin content affects H
2
O
2
accumulation and a pathogen defense in
Arabidopsis thaliana
. Leaf content of free riboflavin was modulated by ectopic expression of the turtle gene encoding riboflavin-binding protein (RfBP). The
RfBP
-expressing
Arabidopsis thaliana
(REAT) plants produced the RfBP protein that possessed riboflavin-binding activity. Compared with the wild-type plant, several tested REAT lines had >70% less flavins of free form. This change accompanied an elevation in the level of H
2
O
2
and an enhancement of plant resistance to a bacterial pathogen. All the observed REAT characters were eliminated due to
RfBP
silencing (RfBPi) under REAT background. When an H
2
O
2
scavenger was applied, H
2
O
2
level declined in all the plants, and REAT no longer exhibited the phenotype of resistance enhancement. However, treatment with an NADPH oxidase inhibitor diminished H
2
O
2
content and pathogen defense in wild-type and RfBPi but not in REAT. Our results suggest that the intrinsic down-regulation of free flavins is responsible for NADPH oxidase-independent H
2
O
2
accumulation and the pathogen defense.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21720919</pmid><doi>10.1007/s11103-011-9802-0</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 2011-09, Vol.77 (1-2), p.185-201 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis - microbiology Arabidopsis Proteins - metabolism Biochemistry Biomedical and Life Sciences Developmental stages Down-Regulation Hydrogen peroxide Hydrogen Peroxide - metabolism Immunity, Innate Life Sciences NADPH Oxidases - metabolism Pathogens Phenotype Plant biology Plant Pathology Plant resistance Plant Sciences Plants, Genetically Modified - metabolism Plants, Genetically Modified - microbiology Proteins Riboflavin - genetics Riboflavin - metabolism Turtles - genetics |
| title | Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis |
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