Effects of salt formulations, carbon sources, cytokinins, and auxin on shoot organogenesis from cotyledons of Pinus pinea L
Adventitious shoots were induced on cotyledons of Pinus pinea. Among seven salt formulations, three carbon sources (sucrose, glucose and fructose), and two cytokinins [6-benzyladenine (BA) and thidiazuron (TDZ)] with and without [α-naphthaleneacetic acid (NAA)], cotyledons grown on 1/2 MS medium con...
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| Veröffentlicht in: | Plant growth regulation 2004-07, Vol.43 (3), p.197-205 |
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| creator | Sul, I.W Korban, S.S |
| description | Adventitious shoots were induced on cotyledons of Pinus pinea. Among seven salt formulations, three carbon sources (sucrose, glucose and fructose), and two cytokinins [6-benzyladenine (BA) and thidiazuron (TDZ)] with and without [α-naphthaleneacetic acid (NAA)], cotyledons grown on 1/2 MS medium containing sucrose and 30 μM BA produced the highest frequency of shoot organogenesis (>90%) and mean number of shoots/explant (>40% of cotyledons produced over 20 shoots/cotyledon). As for the site of shoot organogenesis along the cotyledonary explant, the highest number of shoots per explant was observed along the basal segment of the cotyledon (distal to the hypocotyl) over all cytokinin and auxin treatments tested. Shoots were elongated on a growth regulator-free medium containing activated charcoal.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1023/B:GROW.0000046013.47892.4f |
| format | Article |
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Among seven salt formulations, three carbon sources (sucrose, glucose and fructose), and two cytokinins [6-benzyladenine (BA) and thidiazuron (TDZ)] with and without [α-naphthaleneacetic acid (NAA)], cotyledons grown on 1/2 MS medium containing sucrose and 30 μM BA produced the highest frequency of shoot organogenesis (>90%) and mean number of shoots/explant (>40% of cotyledons produced over 20 shoots/cotyledon). As for the site of shoot organogenesis along the cotyledonary explant, the highest number of shoots per explant was observed along the basal segment of the cotyledon (distal to the hypocotyl) over all cytokinin and auxin treatments tested. Shoots were elongated on a growth regulator-free medium containing activated charcoal.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0167-6903</identifier><identifier>EISSN: 1573-5087</identifier><identifier>DOI: 10.1023/B:GROW.0000046013.47892.4f</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>Activated charcoal ; adventitious shoots ; benzyladenine ; Carbon sources ; Charcoal ; cotyledons ; culture media ; dose response ; forest trees ; fructose ; glucose ; Growth regulators ; in vitro culture ; in vitro regeneration ; methodology ; Microbiology ; micropropagation ; naphthaleneacetic acid ; organogenesis ; Pinus pinea ; sucrose ; thidiazuron</subject><ispartof>Plant growth regulation, 2004-07, Vol.43 (3), p.197-205</ispartof><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-2921de9491041bb6dee8b20180d9ba2c964d3cfb5a7bc00f4fe13cef710e0d4a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids></links><search><creatorcontrib>Sul, I.W</creatorcontrib><creatorcontrib>Korban, S.S</creatorcontrib><title>Effects of salt formulations, carbon sources, cytokinins, and auxin on shoot organogenesis from cotyledons of Pinus pinea L</title><title>Plant growth regulation</title><description>Adventitious shoots were induced on cotyledons of Pinus pinea. Among seven salt formulations, three carbon sources (sucrose, glucose and fructose), and two cytokinins [6-benzyladenine (BA) and thidiazuron (TDZ)] with and without [α-naphthaleneacetic acid (NAA)], cotyledons grown on 1/2 MS medium containing sucrose and 30 μM BA produced the highest frequency of shoot organogenesis (>90%) and mean number of shoots/explant (>40% of cotyledons produced over 20 shoots/cotyledon). As for the site of shoot organogenesis along the cotyledonary explant, the highest number of shoots per explant was observed along the basal segment of the cotyledon (distal to the hypocotyl) over all cytokinin and auxin treatments tested. Shoots were elongated on a growth regulator-free medium containing activated charcoal.[PUBLICATION ABSTRACT]</description><subject>Activated charcoal</subject><subject>adventitious shoots</subject><subject>benzyladenine</subject><subject>Carbon sources</subject><subject>Charcoal</subject><subject>cotyledons</subject><subject>culture media</subject><subject>dose response</subject><subject>forest trees</subject><subject>fructose</subject><subject>glucose</subject><subject>Growth regulators</subject><subject>in vitro culture</subject><subject>in vitro regeneration</subject><subject>methodology</subject><subject>Microbiology</subject><subject>micropropagation</subject><subject>naphthaleneacetic acid</subject><subject>organogenesis</subject><subject>Pinus pinea</subject><subject>sucrose</subject><subject>thidiazuron</subject><issn>0167-6903</issn><issn>1573-5087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpFUNtKAzEQDaJgvXyDoc9unWyyu4lvtniDguIFH0M2m9TUNqnJLlj8eXet4LwMw5zLzEFoTGBCIKcX08vbp4e3CQzFSiB0wiou8gmze2hEiopmBfBqH42AlFVWCqCH6CilZQ_nvCAj9H1trdFtwsHipFYttiGuu5VqXfDpHGsV6-BxCl3UZpi3bfhw3g075Rusui_n8YB4D6HFIS6UDwvjTXIJ2xjWWId2uzJNrzZYPDrfJbxx3ig8P0EHVq2SOf3rx-j15vpldpfNH27vZ1fzTFPC2iwXOWmMYIIAI3VdNsbwOgfCoRG1yrUoWUO1rQtV1RrAMmsI1cZWBAw0TNFjNN7pbmL47Exq5bL_x_eWknMqACpOetDlDqRjSCkaKzfRrVXcSgJyyFpO5ZC1_M9a_mYtme3JZzuyVUGqRXRJvj73J1IAUQEpGP0BiOV-6w</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Sul, I.W</creator><creator>Korban, S.S</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20040701</creationdate><title>Effects of salt formulations, carbon sources, cytokinins, and auxin on shoot organogenesis from cotyledons of Pinus pinea L</title><author>Sul, I.W ; 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| ispartof | Plant growth regulation, 2004-07, Vol.43 (3), p.197-205 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Activated charcoal adventitious shoots benzyladenine Carbon sources Charcoal cotyledons culture media dose response forest trees fructose glucose Growth regulators in vitro culture in vitro regeneration methodology Microbiology micropropagation naphthaleneacetic acid organogenesis Pinus pinea sucrose thidiazuron |
| title | Effects of salt formulations, carbon sources, cytokinins, and auxin on shoot organogenesis from cotyledons of Pinus pinea L |
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