Genetic identification of two species of Pleuronichthys by DNA barcoding
DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in t...
Gespeichert in:
| Veröffentlicht in: | Chinese journal of oceanology and limnology 2011-09, Vol.29 (5), p.967-972 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 972 |
|---|---|
| container_issue | 5 |
| container_start_page | 967 |
| container_title | Chinese journal of oceanology and limnology |
| container_volume | 29 |
| creator | 张辉 张岩 高天翔 李鹏飞 徐汉祥 |
| description | DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments. |
| doi_str_mv | 10.1007/s00343-011-0040-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_881458548</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>38981774</cqvip_id><sourcerecordid>907168932</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-7be981fbab65d956e20a78ebe9fcae43dcdd23708c2914b3734dd70f1154305b3</originalsourceid><addsrcrecordid>eNp9kMFKAzEQhoMoWKsP4G314ml1skma5FiqtkJRD3oOu9lsm7JN2mQX6dub0qLgwdMww_fPDB9C1xjuMQB_iACEkhwwzgEo5OIEDbCUJGcA7BQNoGA0p8DkObqIcZVoSUEO0GxqnOmszmxtXGcbq8vOepf5Juu-fBY3RlsT9-17a_rgndXLbrmLWbXLHl_HWVUG7WvrFpforCnbaK6OdYg-n58-JrN8_jZ9mYznuaZEdjmvjBS4qcpqxGrJRqaAkguTpo0uDSW1ruuCcBC6kJhWhBNa1xwajBklwCoyRHeHvZvgt72JnVrbqE3bls74PioJHI-EJEUib_-QK98Hl55TQmDKBKMiQfgA6eBjDKZRm2DXZdgpDGpvVh3MqmRW7c2qfaY4ZGJi3cKE38X_hW6Oh5beLbYp93OJiKSEc0q-ARkAhdY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>881458548</pqid></control><display><type>article</type><title>Genetic identification of two species of Pleuronichthys by DNA barcoding</title><source>ProQuest Central Essentials</source><source>ProQuest Central (Alumni Edition)</source><source>ProQuest Central Student</source><source>ProQuest Central Korea</source><source>ProQuest Central UK/Ireland</source><source>Alma/SFX Local Collection</source><source>SpringerLink Journals - AutoHoldings</source><source>ProQuest Central</source><creator>张辉 张岩 高天翔 李鹏飞 徐汉祥</creator><creatorcontrib>张辉 张岩 高天翔 李鹏飞 徐汉祥</creatorcontrib><description>DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.</description><identifier>ISSN: 0254-4059</identifier><identifier>ISSN: 2096-5508</identifier><identifier>EISSN: 1993-5005</identifier><identifier>EISSN: 2523-3521</identifier><identifier>DOI: 10.1007/s00343-011-0040-8</identifier><language>eng</language><publisher>Heidelberg: SP Science Press</publisher><subject>Bar codes ; Biology ; Cytochrome ; Cytochrome b ; Cytochrome-c oxidase ; Cytochromes ; Deoxyribonucleic acid ; DNA ; DNA barcoding ; DNA fingerprints ; DNA片段 ; Earth and Environmental Science ; Earth Sciences ; Fragments ; Gene sequencing ; Life history ; Marine ; Mitochondrial DNA ; Morphology ; mtDNA控制区 ; Oceanography ; Pleuronichthys ; Pleuronichthys cornutus ; Species ; Taxonomy ; 条码技术 ; 物种 ; 生物分类学 ; 细胞色素c氧化酶 ; 识别能力 ; 遗传鉴定</subject><ispartof>Chinese journal of oceanology and limnology, 2011-09, Vol.29 (5), p.967-972</ispartof><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2011</rights><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2011.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-7be981fbab65d956e20a78ebe9fcae43dcdd23708c2914b3734dd70f1154305b3</citedby><cites>FETCH-LOGICAL-c439t-7be981fbab65d956e20a78ebe9fcae43dcdd23708c2914b3734dd70f1154305b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84119X/84119X.jpg</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/881458548/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/881458548?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,21386,21387,21388,21389,23254,27922,27923,33528,33701,33742,34003,34312,43657,43785,43803,43951,44065,64383,64387,72239,73874,74053,74072,74243,74360</link.rule.ids></links><search><creatorcontrib>张辉 张岩 高天翔 李鹏飞 徐汉祥</creatorcontrib><title>Genetic identification of two species of Pleuronichthys by DNA barcoding</title><title>Chinese journal of oceanology and limnology</title><addtitle>Chin. J. Ocean. Limnol</addtitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><description>DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.</description><subject>Bar codes</subject><subject>Biology</subject><subject>Cytochrome</subject><subject>Cytochrome b</subject><subject>Cytochrome-c oxidase</subject><subject>Cytochromes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA barcoding</subject><subject>DNA fingerprints</subject><subject>DNA片段</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Fragments</subject><subject>Gene sequencing</subject><subject>Life history</subject><subject>Marine</subject><subject>Mitochondrial DNA</subject><subject>Morphology</subject><subject>mtDNA控制区</subject><subject>Oceanography</subject><subject>Pleuronichthys</subject><subject>Pleuronichthys cornutus</subject><subject>Species</subject><subject>Taxonomy</subject><subject>条码技术</subject><subject>物种</subject><subject>生物分类学</subject><subject>细胞色素c氧化酶</subject><subject>识别能力</subject><subject>遗传鉴定</subject><issn>0254-4059</issn><issn>2096-5508</issn><issn>1993-5005</issn><issn>2523-3521</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMFKAzEQhoMoWKsP4G314ml1skma5FiqtkJRD3oOu9lsm7JN2mQX6dub0qLgwdMww_fPDB9C1xjuMQB_iACEkhwwzgEo5OIEDbCUJGcA7BQNoGA0p8DkObqIcZVoSUEO0GxqnOmszmxtXGcbq8vOepf5Juu-fBY3RlsT9-17a_rgndXLbrmLWbXLHl_HWVUG7WvrFpforCnbaK6OdYg-n58-JrN8_jZ9mYznuaZEdjmvjBS4qcpqxGrJRqaAkguTpo0uDSW1ruuCcBC6kJhWhBNa1xwajBklwCoyRHeHvZvgt72JnVrbqE3bls74PioJHI-EJEUib_-QK98Hl55TQmDKBKMiQfgA6eBjDKZRm2DXZdgpDGpvVh3MqmRW7c2qfaY4ZGJi3cKE38X_hW6Oh5beLbYp93OJiKSEc0q-ARkAhdY</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>张辉 张岩 高天翔 李鹏飞 徐汉祥</creator><general>SP Science Press</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QL</scope><scope>7SN</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7XB</scope><scope>88I</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H96</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>M2P</scope><scope>M7N</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7TM</scope><scope>H95</scope></search><sort><creationdate>20110901</creationdate><title>Genetic identification of two species of Pleuronichthys by DNA barcoding</title><author>张辉 张岩 高天翔 李鹏飞 徐汉祥</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-7be981fbab65d956e20a78ebe9fcae43dcdd23708c2914b3734dd70f1154305b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bar codes</topic><topic>Biology</topic><topic>Cytochrome</topic><topic>Cytochrome b</topic><topic>Cytochrome-c oxidase</topic><topic>Cytochromes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA barcoding</topic><topic>DNA fingerprints</topic><topic>DNA片段</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>Fragments</topic><topic>Gene sequencing</topic><topic>Life history</topic><topic>Marine</topic><topic>Mitochondrial DNA</topic><topic>Morphology</topic><topic>mtDNA控制区</topic><topic>Oceanography</topic><topic>Pleuronichthys</topic><topic>Pleuronichthys cornutus</topic><topic>Species</topic><topic>Taxonomy</topic><topic>条码技术</topic><topic>物种</topic><topic>生物分类学</topic><topic>细胞色素c氧化酶</topic><topic>识别能力</topic><topic>遗传鉴定</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>张辉 张岩 高天翔 李鹏飞 徐汉祥</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Nucleic Acids Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><jtitle>Chinese journal of oceanology and limnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>张辉 张岩 高天翔 李鹏飞 徐汉祥</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic identification of two species of Pleuronichthys by DNA barcoding</atitle><jtitle>Chinese journal of oceanology and limnology</jtitle><stitle>Chin. J. Ocean. Limnol</stitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>29</volume><issue>5</issue><spage>967</spage><epage>972</epage><pages>967-972</pages><issn>0254-4059</issn><issn>2096-5508</issn><eissn>1993-5005</eissn><eissn>2523-3521</eissn><abstract>DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.</abstract><cop>Heidelberg</cop><pub>SP Science Press</pub><doi>10.1007/s00343-011-0040-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0254-4059 |
| ispartof | Chinese journal of oceanology and limnology, 2011-09, Vol.29 (5), p.967-972 |
| issn | 0254-4059 2096-5508 1993-5005 2523-3521 |
| language | eng |
| recordid | cdi_proquest_journals_881458548 |
| source | ProQuest Central Essentials; ProQuest Central (Alumni Edition); ProQuest Central Student; ProQuest Central Korea; ProQuest Central UK/Ireland; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings; ProQuest Central |
| subjects | Bar codes Biology Cytochrome Cytochrome b Cytochrome-c oxidase Cytochromes Deoxyribonucleic acid DNA DNA barcoding DNA fingerprints DNA片段 Earth and Environmental Science Earth Sciences Fragments Gene sequencing Life history Marine Mitochondrial DNA Morphology mtDNA控制区 Oceanography Pleuronichthys Pleuronichthys cornutus Species Taxonomy 条码技术 物种 生物分类学 细胞色素c氧化酶 识别能力 遗传鉴定 |
| title | Genetic identification of two species of Pleuronichthys by DNA barcoding |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T20%3A24%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20identification%20of%20two%20species%20of%20Pleuronichthys%20by%20DNA%20barcoding&rft.jtitle=Chinese%20journal%20of%20oceanology%20and%20limnology&rft.au=%E5%BC%A0%E8%BE%89%20%E5%BC%A0%E5%B2%A9%20%E9%AB%98%E5%A4%A9%E7%BF%94%20%E6%9D%8E%E9%B9%8F%E9%A3%9E%20%E5%BE%90%E6%B1%89%E7%A5%A5&rft.date=2011-09-01&rft.volume=29&rft.issue=5&rft.spage=967&rft.epage=972&rft.pages=967-972&rft.issn=0254-4059&rft.eissn=1993-5005&rft_id=info:doi/10.1007/s00343-011-0040-8&rft_dat=%3Cproquest_cross%3E907168932%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=881458548&rft_id=info:pmid/&rft_cqvip_id=38981774&rfr_iscdi=true |