Loss of Dnd1 facilitates the cultivation of genital ridge-derived rat embryonic germ cells
Pluripotent cells referred to as embryonic germ cells (EGCs) can be derived from the embryonic precursors of the mature gametes: the primordial germ cells (PGCs). A homozygous mutation ( ter) of the dead-end homolog 1 gene ( Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to n...
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| Veröffentlicht in: | Experimental cell research 2011-08, Vol.317 (13), p.1885-1894 |
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| container_title | Experimental cell research |
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| creator | Northrup, Emily Eisenblätter, Regina Glage, Silke Rudolph, Cornelia Dorsch, Martina Schlegelberger, Brigitte Hedrich, Hans-Jürgen Zschemisch, Nils-Holger |
| description | Pluripotent cells referred to as embryonic germ cells (EGCs) can be derived from the embryonic precursors of the mature gametes: the primordial germ cells (PGCs). A homozygous mutation (
ter) of the
dead-end homolog 1 gene (
Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to neoplastic transformation and loss of germ cells. We mated heterozygous
ter/+ WKY-
Dnd1
ter
/Ztm rats and were able to cultivate the first genital ridge-derived EGCs of the rat embryo at day 14.5 post coitum (pc). Genotyping revealed that ten EGC lines were
Dnd1 deficient, while only one wild type cell line had survived in culture. This suggests that the inactivation of the putative tumor suppressor gene
Dnd1 facilitates the immortalization of late EGCs
in vitro. Injection of the wild type EGCs into blastocysts resulted in the first germ-line competent chimeras. These new pluripotent rat EGCs offer an innovative approach for studies on germ cell tumor development as well as a new tool for genetic manipulations in rats. |
| doi_str_mv | 10.1016/j.yexcr.2011.04.013 |
| format | Article |
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ter) of the
dead-end homolog 1 gene (
Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to neoplastic transformation and loss of germ cells. We mated heterozygous
ter/+ WKY-
Dnd1
ter
/Ztm rats and were able to cultivate the first genital ridge-derived EGCs of the rat embryo at day 14.5 post coitum (pc). Genotyping revealed that ten EGC lines were
Dnd1 deficient, while only one wild type cell line had survived in culture. This suggests that the inactivation of the putative tumor suppressor gene
Dnd1 facilitates the immortalization of late EGCs
in vitro. Injection of the wild type EGCs into blastocysts resulted in the first germ-line competent chimeras. These new pluripotent rat EGCs offer an innovative approach for studies on germ cell tumor development as well as a new tool for genetic manipulations in rats.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2011.04.013</identifier><identifier>PMID: 21570390</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cells, Cultured ; Cellular biology ; Dnd1 ; Embryonic germ cell ; Female ; Genes, Tumor Suppressor ; Genotype & phenotype ; Germ Cells - cytology ; Germ Cells - metabolism ; Germ-line competent chimera ; Male ; Population genetics ; Primordial germ cell ; Rat ; Rats ; Rats, Inbred Strains ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; Rodents ; Teratoma ; Tumor Suppressor Proteins - deficiency ; Tumor Suppressor Proteins - genetics ; Tumor Suppressor Proteins - metabolism ; Tumors</subject><ispartof>Experimental cell research, 2011-08, Vol.317 (13), p.1885-1894</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-bfa6fd79369057cb3e38f8718ce4250eb4dab8d65a2ca556553aabf96d08364f3</citedby><cites>FETCH-LOGICAL-c451t-bfa6fd79369057cb3e38f8718ce4250eb4dab8d65a2ca556553aabf96d08364f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexcr.2011.04.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21570390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Northrup, Emily</creatorcontrib><creatorcontrib>Eisenblätter, Regina</creatorcontrib><creatorcontrib>Glage, Silke</creatorcontrib><creatorcontrib>Rudolph, Cornelia</creatorcontrib><creatorcontrib>Dorsch, Martina</creatorcontrib><creatorcontrib>Schlegelberger, Brigitte</creatorcontrib><creatorcontrib>Hedrich, Hans-Jürgen</creatorcontrib><creatorcontrib>Zschemisch, Nils-Holger</creatorcontrib><title>Loss of Dnd1 facilitates the cultivation of genital ridge-derived rat embryonic germ cells</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Pluripotent cells referred to as embryonic germ cells (EGCs) can be derived from the embryonic precursors of the mature gametes: the primordial germ cells (PGCs). A homozygous mutation (
ter) of the
dead-end homolog 1 gene (
Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to neoplastic transformation and loss of germ cells. We mated heterozygous
ter/+ WKY-
Dnd1
ter
/Ztm rats and were able to cultivate the first genital ridge-derived EGCs of the rat embryo at day 14.5 post coitum (pc). Genotyping revealed that ten EGC lines were
Dnd1 deficient, while only one wild type cell line had survived in culture. This suggests that the inactivation of the putative tumor suppressor gene
Dnd1 facilitates the immortalization of late EGCs
in vitro. Injection of the wild type EGCs into blastocysts resulted in the first germ-line competent chimeras. These new pluripotent rat EGCs offer an innovative approach for studies on germ cell tumor development as well as a new tool for genetic manipulations in rats.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Dnd1</subject><subject>Embryonic germ cell</subject><subject>Female</subject><subject>Genes, Tumor Suppressor</subject><subject>Genotype & phenotype</subject><subject>Germ Cells - cytology</subject><subject>Germ Cells - metabolism</subject><subject>Germ-line competent chimera</subject><subject>Male</subject><subject>Population genetics</subject><subject>Primordial germ cell</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Rodents</subject><subject>Teratoma</subject><subject>Tumor Suppressor Proteins - deficiency</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumor Suppressor Proteins - metabolism</subject><subject>Tumors</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD2PEzEQhi0E4sLBL0BCFv0u48_1FhToji8pEg00NJbXHh-ONruH7UTk3-OQg5JqinlmXr0PIS8Z9AyYfrPrT_jL554DYz3IHph4RDYMRui45Pwx2QAw2UnDhyvyrJQdABjD9FNyxZkaQIywId-3ayl0jfR2CYxG59OcqqtYaP2B1B_mmo6upnU5M3e4tOVMcwp32AXM6YiBZlcp7qd8WpfkG5P31OM8l-fkSXRzwRcP85p8-_D-682nbvvl4-ebd9vOS8VqN0WnYxhGoUdQg58EChPNwIxHyRXgJIObTNDKce-U0koJ56Y46gBGaBnFNXl9-Xuf158HLNXu1kNeWqQ1gxhg5HpskLhAPrfCGaO9z2nv8skysGeddmf_6LRnnRakbTrb1auH14dpj-HfzV9_DXh7AbAVPCbMtviEi8eQMvpqw5r-G_AbBtmHpw</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>Northrup, Emily</creator><creator>Eisenblätter, Regina</creator><creator>Glage, Silke</creator><creator>Rudolph, Cornelia</creator><creator>Dorsch, Martina</creator><creator>Schlegelberger, Brigitte</creator><creator>Hedrich, Hans-Jürgen</creator><creator>Zschemisch, Nils-Holger</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110801</creationdate><title>Loss of Dnd1 facilitates the cultivation of genital ridge-derived rat embryonic germ cells</title><author>Northrup, Emily ; Eisenblätter, Regina ; Glage, Silke ; Rudolph, Cornelia ; Dorsch, Martina ; Schlegelberger, Brigitte ; Hedrich, Hans-Jürgen ; Zschemisch, Nils-Holger</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-bfa6fd79369057cb3e38f8718ce4250eb4dab8d65a2ca556553aabf96d08364f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Dnd1</topic><topic>Embryonic germ cell</topic><topic>Female</topic><topic>Genes, Tumor Suppressor</topic><topic>Genotype & phenotype</topic><topic>Germ Cells - cytology</topic><topic>Germ Cells - metabolism</topic><topic>Germ-line competent chimera</topic><topic>Male</topic><topic>Population genetics</topic><topic>Primordial germ cell</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Rodents</topic><topic>Teratoma</topic><topic>Tumor Suppressor Proteins - deficiency</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Tumor Suppressor Proteins - metabolism</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Northrup, Emily</creatorcontrib><creatorcontrib>Eisenblätter, Regina</creatorcontrib><creatorcontrib>Glage, Silke</creatorcontrib><creatorcontrib>Rudolph, Cornelia</creatorcontrib><creatorcontrib>Dorsch, Martina</creatorcontrib><creatorcontrib>Schlegelberger, Brigitte</creatorcontrib><creatorcontrib>Hedrich, Hans-Jürgen</creatorcontrib><creatorcontrib>Zschemisch, Nils-Holger</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Northrup, Emily</au><au>Eisenblätter, Regina</au><au>Glage, Silke</au><au>Rudolph, Cornelia</au><au>Dorsch, Martina</au><au>Schlegelberger, Brigitte</au><au>Hedrich, Hans-Jürgen</au><au>Zschemisch, Nils-Holger</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Loss of Dnd1 facilitates the cultivation of genital ridge-derived rat embryonic germ cells</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>317</volume><issue>13</issue><spage>1885</spage><epage>1894</epage><pages>1885-1894</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Pluripotent cells referred to as embryonic germ cells (EGCs) can be derived from the embryonic precursors of the mature gametes: the primordial germ cells (PGCs). A homozygous mutation (
ter) of the
dead-end homolog 1 gene (
Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to neoplastic transformation and loss of germ cells. We mated heterozygous
ter/+ WKY-
Dnd1
ter
/Ztm rats and were able to cultivate the first genital ridge-derived EGCs of the rat embryo at day 14.5 post coitum (pc). Genotyping revealed that ten EGC lines were
Dnd1 deficient, while only one wild type cell line had survived in culture. This suggests that the inactivation of the putative tumor suppressor gene
Dnd1 facilitates the immortalization of late EGCs
in vitro. Injection of the wild type EGCs into blastocysts resulted in the first germ-line competent chimeras. These new pluripotent rat EGCs offer an innovative approach for studies on germ cell tumor development as well as a new tool for genetic manipulations in rats.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21570390</pmid><doi>10.1016/j.yexcr.2011.04.013</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Cells, Cultured Cellular biology Dnd1 Embryonic germ cell Female Genes, Tumor Suppressor Genotype & phenotype Germ Cells - cytology Germ Cells - metabolism Germ-line competent chimera Male Population genetics Primordial germ cell Rat Rats Rats, Inbred Strains RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Rodents Teratoma Tumor Suppressor Proteins - deficiency Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - metabolism Tumors |
| title | Loss of Dnd1 facilitates the cultivation of genital ridge-derived rat embryonic germ cells |
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