Determination of Cell Proliferation Using Mcm2 Antigen and Evaluation of Apoptosis and TGF-[beta]1 Expression in GH-secreting or Clinically Nonfunctioning Pituitary Adenomas

Determination of Cell Proliferation Using Mcm2 Antigen and Evaluation of Apoptosis and TGF-[beta]1 Expression in GH-secreting or Clinically Nonfunctioning Pituitary Adenomas

Pituitary adenomas (PA) occasionally show aggressive behavior, with invasion of the surrounding tissues. The identification of markers able to recognize aggressive PA in early stages remains a challenge. We aimed to determine the expression of a new cell proliferation marker, Mcm2, and the presence...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Endocrine pathology 2010-03, Vol.21 (1), p.32
Hauptverfasser: Dallago, Cristina Micheletto, Barbosa-coutinho, Ligia Maria, Ferreira, Nelson Pires, Meurer, Rosalva, Pereira-lima, Julia Fernanda, Semmelmann, Da Costa Oliveira, Miriam
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 1
container_start_page 32
container_title Endocrine pathology
container_volume 21
creator Dallago, Cristina Micheletto
Barbosa-coutinho, Ligia Maria
Ferreira, Nelson Pires
Meurer, Rosalva
Pereira-lima, Julia Fernanda
Semmelmann
Da Costa Oliveira, Miriam
description Pituitary adenomas (PA) occasionally show aggressive behavior, with invasion of the surrounding tissues. The identification of markers able to recognize aggressive PA in early stages remains a challenge. We aimed to determine the expression of a new cell proliferation marker, Mcm2, and the presence of apoptosis in PA, and to evaluate the association of clinicopathological features with the apoptotic and proliferative indices. Additionally, the TGF-β1 expression, an inducer of apoptosis, was determined. The proliferative index was determined in GH-secreting or clinically nonfunctioning PA using immunohistochemical (IH) methods for Mcm2 and Ki-67 antigens. The apoptosis was assessed by the TUNEL method and the TGF-β1 expression by IH. A significant positive correlation was found between log Mcm2 index and log Ki-67 index (p
doi_str_mv 10.1007/s12022-010-9107-x
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_872076729</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2376349781</sourcerecordid><originalsourceid>FETCH-proquest_journals_8720767293</originalsourceid><addsrcrecordid>eNqNjMtOwzAURC0EEuXxAews9oZrh8ZkGYU-NqAuygqhyqQ3lSvnOtgOaj-Kf6QBxJrVjObMDGNXEm4kgL6NUoFSAiSIQoIWuyM2kuNxIQAKfXzwcJeLrND5KTuLcQsgMwA1Yp8PmDC0lkyynrhveIXO8UXwzjYYftLnaGnDH-tW8ZKS3SBxQ2s--TCu_9uVne-SjzZ-s-VsKl7eMJlXySe7LmCMQ88Sn81FxDpgGj594JWzZGvj3J4_eWp6qofHAS5s6m0yYc_LNZJvTbxgJ41xES9_9ZxdTyfLai664N97jGm19X2gA1rdawU616rI_lX6AvWLZ08</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>872076729</pqid></control><display><type>article</type><title>Determination of Cell Proliferation Using Mcm2 Antigen and Evaluation of Apoptosis and TGF-[beta]1 Expression in GH-secreting or Clinically Nonfunctioning Pituitary Adenomas</title><source>SpringerLink Journals - AutoHoldings</source><creator>Dallago, Cristina Micheletto ; Barbosa-coutinho, Ligia Maria ; Ferreira, Nelson Pires ; Meurer, Rosalva ; Pereira-lima, Julia Fernanda; Semmelmann ; Da Costa Oliveira, Miriam</creator><creatorcontrib>Dallago, Cristina Micheletto ; Barbosa-coutinho, Ligia Maria ; Ferreira, Nelson Pires ; Meurer, Rosalva ; Pereira-lima, Julia Fernanda; Semmelmann ; Da Costa Oliveira, Miriam</creatorcontrib><description>Pituitary adenomas (PA) occasionally show aggressive behavior, with invasion of the surrounding tissues. The identification of markers able to recognize aggressive PA in early stages remains a challenge. We aimed to determine the expression of a new cell proliferation marker, Mcm2, and the presence of apoptosis in PA, and to evaluate the association of clinicopathological features with the apoptotic and proliferative indices. Additionally, the TGF-β1 expression, an inducer of apoptosis, was determined. The proliferative index was determined in GH-secreting or clinically nonfunctioning PA using immunohistochemical (IH) methods for Mcm2 and Ki-67 antigens. The apoptosis was assessed by the TUNEL method and the TGF-β1 expression by IH. A significant positive correlation was found between log Mcm2 index and log Ki-67 index (p&lt;0.001). Mcm2 and Ki-67 detected a similar number of proliferating cells. Mcm2 index showed a significant association with tumor extension (p=0.02), but not with tumor invasion. Apoptosis was detected in 17% of the adenomas, with a maximum apoptotic index of 0.77%. Immunoreactivity to TGF-β1 was observed in 77% of the adenomas, showing an association with tumor extension. We concluded that, in this sample, Mcm2 was similar to Ki-67 in the identification of the proliferating cells and that apoptosis was rare.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 1046-3976</identifier><identifier>EISSN: 1559-0097</identifier><identifier>DOI: 10.1007/s12022-010-9107-x</identifier><language>eng</language><publisher>Cambridge: Springer Nature B.V</publisher><ispartof>Endocrine pathology, 2010-03, Vol.21 (1), p.32</ispartof><rights>Springer Science+Business Media, LLC 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Dallago, Cristina Micheletto</creatorcontrib><creatorcontrib>Barbosa-coutinho, Ligia Maria</creatorcontrib><creatorcontrib>Ferreira, Nelson Pires</creatorcontrib><creatorcontrib>Meurer, Rosalva</creatorcontrib><creatorcontrib>Pereira-lima, Julia Fernanda; Semmelmann</creatorcontrib><creatorcontrib>Da Costa Oliveira, Miriam</creatorcontrib><title>Determination of Cell Proliferation Using Mcm2 Antigen and Evaluation of Apoptosis and TGF-[beta]1 Expression in GH-secreting or Clinically Nonfunctioning Pituitary Adenomas</title><title>Endocrine pathology</title><description>Pituitary adenomas (PA) occasionally show aggressive behavior, with invasion of the surrounding tissues. The identification of markers able to recognize aggressive PA in early stages remains a challenge. We aimed to determine the expression of a new cell proliferation marker, Mcm2, and the presence of apoptosis in PA, and to evaluate the association of clinicopathological features with the apoptotic and proliferative indices. Additionally, the TGF-β1 expression, an inducer of apoptosis, was determined. The proliferative index was determined in GH-secreting or clinically nonfunctioning PA using immunohistochemical (IH) methods for Mcm2 and Ki-67 antigens. The apoptosis was assessed by the TUNEL method and the TGF-β1 expression by IH. A significant positive correlation was found between log Mcm2 index and log Ki-67 index (p&lt;0.001). Mcm2 and Ki-67 detected a similar number of proliferating cells. Mcm2 index showed a significant association with tumor extension (p=0.02), but not with tumor invasion. Apoptosis was detected in 17% of the adenomas, with a maximum apoptotic index of 0.77%. Immunoreactivity to TGF-β1 was observed in 77% of the adenomas, showing an association with tumor extension. We concluded that, in this sample, Mcm2 was similar to Ki-67 in the identification of the proliferating cells and that apoptosis was rare.[PUBLICATION ABSTRACT]</description><issn>1046-3976</issn><issn>1559-0097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNjMtOwzAURC0EEuXxAews9oZrh8ZkGYU-NqAuygqhyqQ3lSvnOtgOaj-Kf6QBxJrVjObMDGNXEm4kgL6NUoFSAiSIQoIWuyM2kuNxIQAKfXzwcJeLrND5KTuLcQsgMwA1Yp8PmDC0lkyynrhveIXO8UXwzjYYftLnaGnDH-tW8ZKS3SBxQ2s--TCu_9uVne-SjzZ-s-VsKl7eMJlXySe7LmCMQ88Sn81FxDpgGj594JWzZGvj3J4_eWp6qofHAS5s6m0yYc_LNZJvTbxgJ41xES9_9ZxdTyfLai664N97jGm19X2gA1rdawU616rI_lX6AvWLZ08</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Dallago, Cristina Micheletto</creator><creator>Barbosa-coutinho, Ligia Maria</creator><creator>Ferreira, Nelson Pires</creator><creator>Meurer, Rosalva</creator><creator>Pereira-lima, Julia Fernanda; Semmelmann</creator><creator>Da Costa Oliveira, Miriam</creator><general>Springer Nature B.V</general><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20100301</creationdate><title>Determination of Cell Proliferation Using Mcm2 Antigen and Evaluation of Apoptosis and TGF-[beta]1 Expression in GH-secreting or Clinically Nonfunctioning Pituitary Adenomas</title><author>Dallago, Cristina Micheletto ; Barbosa-coutinho, Ligia Maria ; Ferreira, Nelson Pires ; Meurer, Rosalva ; Pereira-lima, Julia Fernanda; Semmelmann ; Da Costa Oliveira, Miriam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_8720767293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dallago, Cristina Micheletto</creatorcontrib><creatorcontrib>Barbosa-coutinho, Ligia Maria</creatorcontrib><creatorcontrib>Ferreira, Nelson Pires</creatorcontrib><creatorcontrib>Meurer, Rosalva</creatorcontrib><creatorcontrib>Pereira-lima, Julia Fernanda; Semmelmann</creatorcontrib><creatorcontrib>Da Costa Oliveira, Miriam</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Endocrine pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dallago, Cristina Micheletto</au><au>Barbosa-coutinho, Ligia Maria</au><au>Ferreira, Nelson Pires</au><au>Meurer, Rosalva</au><au>Pereira-lima, Julia Fernanda; Semmelmann</au><au>Da Costa Oliveira, Miriam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Cell Proliferation Using Mcm2 Antigen and Evaluation of Apoptosis and TGF-[beta]1 Expression in GH-secreting or Clinically Nonfunctioning Pituitary Adenomas</atitle><jtitle>Endocrine pathology</jtitle><date>2010-03-01</date><risdate>2010</risdate><volume>21</volume><issue>1</issue><spage>32</spage><pages>32-</pages><issn>1046-3976</issn><eissn>1559-0097</eissn><abstract>Pituitary adenomas (PA) occasionally show aggressive behavior, with invasion of the surrounding tissues. The identification of markers able to recognize aggressive PA in early stages remains a challenge. We aimed to determine the expression of a new cell proliferation marker, Mcm2, and the presence of apoptosis in PA, and to evaluate the association of clinicopathological features with the apoptotic and proliferative indices. Additionally, the TGF-β1 expression, an inducer of apoptosis, was determined. The proliferative index was determined in GH-secreting or clinically nonfunctioning PA using immunohistochemical (IH) methods for Mcm2 and Ki-67 antigens. The apoptosis was assessed by the TUNEL method and the TGF-β1 expression by IH. A significant positive correlation was found between log Mcm2 index and log Ki-67 index (p&lt;0.001). Mcm2 and Ki-67 detected a similar number of proliferating cells. Mcm2 index showed a significant association with tumor extension (p=0.02), but not with tumor invasion. Apoptosis was detected in 17% of the adenomas, with a maximum apoptotic index of 0.77%. Immunoreactivity to TGF-β1 was observed in 77% of the adenomas, showing an association with tumor extension. We concluded that, in this sample, Mcm2 was similar to Ki-67 in the identification of the proliferating cells and that apoptosis was rare.[PUBLICATION ABSTRACT]</abstract><cop>Cambridge</cop><pub>Springer Nature B.V</pub><doi>10.1007/s12022-010-9107-x</doi></addata></record>
fulltext fulltext
identifier ISSN: 1046-3976
ispartof Endocrine pathology, 2010-03, Vol.21 (1), p.32
issn 1046-3976
1559-0097
language eng
recordid cdi_proquest_journals_872076729
source SpringerLink Journals - AutoHoldings
title Determination of Cell Proliferation Using Mcm2 Antigen and Evaluation of Apoptosis and TGF-[beta]1 Expression in GH-secreting or Clinically Nonfunctioning Pituitary Adenomas
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T01%3A55%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20Cell%20Proliferation%20Using%20Mcm2%20Antigen%20and%20Evaluation%20of%20Apoptosis%20and%20TGF-%5Bbeta%5D1%20Expression%20in%20GH-secreting%20or%20Clinically%20Nonfunctioning%20Pituitary%20Adenomas&rft.jtitle=Endocrine%20pathology&rft.au=Dallago,%20Cristina%20Micheletto&rft.date=2010-03-01&rft.volume=21&rft.issue=1&rft.spage=32&rft.pages=32-&rft.issn=1046-3976&rft.eissn=1559-0097&rft_id=info:doi/10.1007/s12022-010-9107-x&rft_dat=%3Cproquest%3E2376349781%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=872076729&rft_id=info:pmid/&rfr_iscdi=true