Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations
Methotrexate-human serum albumin conjugates were developed by a simple carbodiimide reaction. Methotrexate-human serum albumin conjugates were then crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) to form nanoparticles. The size of nanoparticles determined by laser light sca...
Gespeichert in:
| Veröffentlicht in: | Journal of nanomaterials 2011-01, Vol.2011 (2011), p.1-7 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7 |
|---|---|
| container_issue | 2011 |
| container_start_page | 1 |
| container_title | Journal of nanomaterials |
| container_volume | 2011 |
| creator | Taheri, Azade Atyabi, Fatemeh Salman Nouri, Faranak Ahadi, Fatemeh Derakhshan, Mohammad Ali Amini, Mohsen Ghahremani, Mohammad Hossein Ostad, Seyed Nasser Mansoori, Pooria Dinarvand, Rassoul |
| description | Methotrexate-human serum albumin conjugates were developed by a simple carbodiimide reaction. Methotrexate-human serum albumin conjugates were then crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) to form nanoparticles. The size of nanoparticles determined by laser light scattering and TEM was between 90–150 nm. Nanoparticles were very stable at physiologic conditions (PBS pH 7.4, 37°C) and after incubation with serum. The effect of amount of EDC used for crosslinking on the particle size and free amino groups of nanoparticles was examined. The amount of crosslinker showed no significant effect on the size of nanoparticles but free amino groups of nanoparticles were decreased by increasing the crosslinker. The physicochemical interactions between methotrexate and human serum albumin were investigated by differential scanning calorimetry (DSC). Nanoparticles were more cytotoxic on T47D cells compared to free methotrexate. Moreover, methotrexate-human serum albumin nanoparticles decreased the IC50 value of methotrexate on T47D cells in comparison with free methotrexate. |
| doi_str_mv | 10.1155/2011/768201 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_856041641</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2287044231</sourcerecordid><originalsourceid>FETCH-LOGICAL-a427t-5ac1489e6e30efef44b52f40de8d1306be3f7a1c0b805b51c2ee3658776c14f03</originalsourceid><addsrcrecordid>eNqF0M9LwzAUB_AgCs7pybMQPCp1SZv0hzcp0wnzB6jnkrYvW0bbzCTV7b83s7Krp5dHPu89-CJ0TskNpZxPQkLpJIlTXw_QiMZpEjAaZof7NyXH6MTaFSGMZzwcocWz6PRaGKeqBizWEue6W_UL4aDGT-CW2hnY-C6Y9a3o8BuYvsV3Tdm3qsO3-NWAnxZO6Q6Lrsb51mmnN6pSbounX6Lpf__sKTqSorFw9lfH6ON--p7PgvnLw2N-Nw8ECxMXcFFRlmYQQ0RAgmSs5KFkpIa0phGJS4hkImhFypTwktMqBIhiniZJ7Aclicbocti7NvqzB-uKle5N508WKY8JozGjHl0PqDLaWgOyWBvVCrMtKCl2QRa7IIshSK-vBr1UXS2-1T_4YsDgCUixxyxLwjCKfgDakn0a</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>856041641</pqid></control><display><type>article</type><title>Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations</title><source>Wiley Online Library Open Access</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Taheri, Azade ; Atyabi, Fatemeh ; Salman Nouri, Faranak ; Ahadi, Fatemeh ; Derakhshan, Mohammad Ali ; Amini, Mohsen ; Ghahremani, Mohammad Hossein ; Ostad, Seyed Nasser ; Mansoori, Pooria ; Dinarvand, Rassoul</creator><contributor>Wu, Libo</contributor><creatorcontrib>Taheri, Azade ; Atyabi, Fatemeh ; Salman Nouri, Faranak ; Ahadi, Fatemeh ; Derakhshan, Mohammad Ali ; Amini, Mohsen ; Ghahremani, Mohammad Hossein ; Ostad, Seyed Nasser ; Mansoori, Pooria ; Dinarvand, Rassoul ; Wu, Libo</creatorcontrib><description>Methotrexate-human serum albumin conjugates were developed by a simple carbodiimide reaction. Methotrexate-human serum albumin conjugates were then crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) to form nanoparticles. The size of nanoparticles determined by laser light scattering and TEM was between 90–150 nm. Nanoparticles were very stable at physiologic conditions (PBS pH 7.4, 37°C) and after incubation with serum. The effect of amount of EDC used for crosslinking on the particle size and free amino groups of nanoparticles was examined. The amount of crosslinker showed no significant effect on the size of nanoparticles but free amino groups of nanoparticles were decreased by increasing the crosslinker. The physicochemical interactions between methotrexate and human serum albumin were investigated by differential scanning calorimetry (DSC). Nanoparticles were more cytotoxic on T47D cells compared to free methotrexate. Moreover, methotrexate-human serum albumin nanoparticles decreased the IC50 value of methotrexate on T47D cells in comparison with free methotrexate.</description><identifier>ISSN: 1687-4110</identifier><identifier>EISSN: 1687-4129</identifier><identifier>DOI: 10.1155/2011/768201</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Cancer ; Cancer therapies ; Medical research ; Nanomaterials ; Pore size ; Proteins</subject><ispartof>Journal of nanomaterials, 2011-01, Vol.2011 (2011), p.1-7</ispartof><rights>Copyright © 2011 Azade Taheri et al.</rights><rights>Copyright © 2011 Azade Taheri et al. Azade Taheri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a427t-5ac1489e6e30efef44b52f40de8d1306be3f7a1c0b805b51c2ee3658776c14f03</citedby><cites>FETCH-LOGICAL-a427t-5ac1489e6e30efef44b52f40de8d1306be3f7a1c0b805b51c2ee3658776c14f03</cites><orcidid>0000-0003-0694-7556 ; 0000-0001-5051-6214</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><contributor>Wu, Libo</contributor><creatorcontrib>Taheri, Azade</creatorcontrib><creatorcontrib>Atyabi, Fatemeh</creatorcontrib><creatorcontrib>Salman Nouri, Faranak</creatorcontrib><creatorcontrib>Ahadi, Fatemeh</creatorcontrib><creatorcontrib>Derakhshan, Mohammad Ali</creatorcontrib><creatorcontrib>Amini, Mohsen</creatorcontrib><creatorcontrib>Ghahremani, Mohammad Hossein</creatorcontrib><creatorcontrib>Ostad, Seyed Nasser</creatorcontrib><creatorcontrib>Mansoori, Pooria</creatorcontrib><creatorcontrib>Dinarvand, Rassoul</creatorcontrib><title>Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations</title><title>Journal of nanomaterials</title><description>Methotrexate-human serum albumin conjugates were developed by a simple carbodiimide reaction. Methotrexate-human serum albumin conjugates were then crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) to form nanoparticles. The size of nanoparticles determined by laser light scattering and TEM was between 90–150 nm. Nanoparticles were very stable at physiologic conditions (PBS pH 7.4, 37°C) and after incubation with serum. The effect of amount of EDC used for crosslinking on the particle size and free amino groups of nanoparticles was examined. The amount of crosslinker showed no significant effect on the size of nanoparticles but free amino groups of nanoparticles were decreased by increasing the crosslinker. The physicochemical interactions between methotrexate and human serum albumin were investigated by differential scanning calorimetry (DSC). Nanoparticles were more cytotoxic on T47D cells compared to free methotrexate. Moreover, methotrexate-human serum albumin nanoparticles decreased the IC50 value of methotrexate on T47D cells in comparison with free methotrexate.</description><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Medical research</subject><subject>Nanomaterials</subject><subject>Pore size</subject><subject>Proteins</subject><issn>1687-4110</issn><issn>1687-4129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0M9LwzAUB_AgCs7pybMQPCp1SZv0hzcp0wnzB6jnkrYvW0bbzCTV7b83s7Krp5dHPu89-CJ0TskNpZxPQkLpJIlTXw_QiMZpEjAaZof7NyXH6MTaFSGMZzwcocWz6PRaGKeqBizWEue6W_UL4aDGT-CW2hnY-C6Y9a3o8BuYvsV3Tdm3qsO3-NWAnxZO6Q6Lrsb51mmnN6pSbounX6Lpf__sKTqSorFw9lfH6ON--p7PgvnLw2N-Nw8ECxMXcFFRlmYQQ0RAgmSs5KFkpIa0phGJS4hkImhFypTwktMqBIhiniZJ7Aclicbocti7NvqzB-uKle5N508WKY8JozGjHl0PqDLaWgOyWBvVCrMtKCl2QRa7IIshSK-vBr1UXS2-1T_4YsDgCUixxyxLwjCKfgDakn0a</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Taheri, Azade</creator><creator>Atyabi, Fatemeh</creator><creator>Salman Nouri, Faranak</creator><creator>Ahadi, Fatemeh</creator><creator>Derakhshan, Mohammad Ali</creator><creator>Amini, Mohsen</creator><creator>Ghahremani, Mohammad Hossein</creator><creator>Ostad, Seyed Nasser</creator><creator>Mansoori, Pooria</creator><creator>Dinarvand, Rassoul</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0003-0694-7556</orcidid><orcidid>https://orcid.org/0000-0001-5051-6214</orcidid></search><sort><creationdate>20110101</creationdate><title>Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations</title><author>Taheri, Azade ; Atyabi, Fatemeh ; Salman Nouri, Faranak ; Ahadi, Fatemeh ; Derakhshan, Mohammad Ali ; Amini, Mohsen ; Ghahremani, Mohammad Hossein ; Ostad, Seyed Nasser ; Mansoori, Pooria ; Dinarvand, Rassoul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a427t-5ac1489e6e30efef44b52f40de8d1306be3f7a1c0b805b51c2ee3658776c14f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Medical research</topic><topic>Nanomaterials</topic><topic>Pore size</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taheri, Azade</creatorcontrib><creatorcontrib>Atyabi, Fatemeh</creatorcontrib><creatorcontrib>Salman Nouri, Faranak</creatorcontrib><creatorcontrib>Ahadi, Fatemeh</creatorcontrib><creatorcontrib>Derakhshan, Mohammad Ali</creatorcontrib><creatorcontrib>Amini, Mohsen</creatorcontrib><creatorcontrib>Ghahremani, Mohammad Hossein</creatorcontrib><creatorcontrib>Ostad, Seyed Nasser</creatorcontrib><creatorcontrib>Mansoori, Pooria</creatorcontrib><creatorcontrib>Dinarvand, Rassoul</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of nanomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taheri, Azade</au><au>Atyabi, Fatemeh</au><au>Salman Nouri, Faranak</au><au>Ahadi, Fatemeh</au><au>Derakhshan, Mohammad Ali</au><au>Amini, Mohsen</au><au>Ghahremani, Mohammad Hossein</au><au>Ostad, Seyed Nasser</au><au>Mansoori, Pooria</au><au>Dinarvand, Rassoul</au><au>Wu, Libo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations</atitle><jtitle>Journal of nanomaterials</jtitle><date>2011-01-01</date><risdate>2011</risdate><volume>2011</volume><issue>2011</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1687-4110</issn><eissn>1687-4129</eissn><abstract>Methotrexate-human serum albumin conjugates were developed by a simple carbodiimide reaction. Methotrexate-human serum albumin conjugates were then crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) to form nanoparticles. The size of nanoparticles determined by laser light scattering and TEM was between 90–150 nm. Nanoparticles were very stable at physiologic conditions (PBS pH 7.4, 37°C) and after incubation with serum. The effect of amount of EDC used for crosslinking on the particle size and free amino groups of nanoparticles was examined. The amount of crosslinker showed no significant effect on the size of nanoparticles but free amino groups of nanoparticles were decreased by increasing the crosslinker. The physicochemical interactions between methotrexate and human serum albumin were investigated by differential scanning calorimetry (DSC). Nanoparticles were more cytotoxic on T47D cells compared to free methotrexate. Moreover, methotrexate-human serum albumin nanoparticles decreased the IC50 value of methotrexate on T47D cells in comparison with free methotrexate.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><doi>10.1155/2011/768201</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0694-7556</orcidid><orcidid>https://orcid.org/0000-0001-5051-6214</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1687-4110 |
| ispartof | Journal of nanomaterials, 2011-01, Vol.2011 (2011), p.1-7 |
| issn | 1687-4110 1687-4129 |
| language | eng |
| recordid | cdi_proquest_journals_856041641 |
| source | Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Cancer Cancer therapies Medical research Nanomaterials Pore size Proteins |
| title | Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T01%3A46%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanoparticles%20of%20Conjugated%20Methotrexate-Human%20Serum%20Albumin%20:%20Preparation%20and%20Cytotoxicity%20Evaluations&rft.jtitle=Journal%20of%20nanomaterials&rft.au=Taheri,%20Azade&rft.date=2011-01-01&rft.volume=2011&rft.issue=2011&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.issn=1687-4110&rft.eissn=1687-4129&rft_id=info:doi/10.1155/2011/768201&rft_dat=%3Cproquest_cross%3E2287044231%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=856041641&rft_id=info:pmid/&rfr_iscdi=true |