Role for PKC in Fenretinide-Mediated Apoptosis in Lymphoid Leukemia Cells
The synthetic Vitamin A analog fenretinide is a promising chemotherapeutic agent. In the current paper, the role of PKC was examined in fenretinide-induced apoptosis in lymphoid leukemia cells. Levels of proapoptotic cleaved PKC positively correlated with drug sensitivity. Fenretinide promoted react...
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| Veröffentlicht in: | Journal of signal transduction 2010-06, Vol.2010, p.1-15 |
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| container_title | Journal of signal transduction |
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| creator | Ruvolo, Vivian R. Karanjeet, Kul B. Schuster, Todd F. Brown, Rhoderick Deng, Yibin Hinchcliffe, Edward Ruvolo, Peter P. |
| description | The synthetic Vitamin A analog fenretinide is a promising chemotherapeutic agent. In the current paper, the role of PKC was examined in fenretinide-induced apoptosis in lymphoid leukemia cells. Levels of proapoptotic cleaved PKC positively correlated with drug sensitivity. Fenretinide promoted reactive oxygen species (ROS) generation. The antioxidant Vitamin C prevented fenretinide-induced PKC cleavage and protected cells from fenretinide. Suppression of PKC expression by shRNA sensitized cells to fenretinide-induced apoptosis possibly by a mechanism involving ROS production. A previous study demonstrated that fenretinide promotes degradation of antiapoptotic MCL-1 in ALL cells via JNK. Now we have found that fenretinide-induced MCL-1 degradation may involve PKC as cleavage of the kinase correlated with loss of MCL-1 even in cells when JNK was not activated. These results suggest that PKC may play a complex role in fenretinide-induced apoptosis and may be targeted in antileukemia strategies that utilize fenretinide. |
| doi_str_mv | 10.1155/2010/584657 |
| format | Article |
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| subjects | Apoptosis Cancer Kinases Proteins |
| title | Role for PKC in Fenretinide-Mediated Apoptosis in Lymphoid Leukemia Cells |
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