Transformed tobacco (Nicotiana tabacum) plants over-expressing a peroxisome proliferator-activated receptor gene from Xenopus laevis (xPPARα) show increased susceptibility to infection by virulent Pseudomonas syringae pathogens
Transgenic tobacco plants capable of over-expressing Xenopus PPARα (xPPARα), a transcription factor known to be required for peroxisome proliferation in animals, were recently generated. These plants (herewith referred to as PPAR-OE) were found to have increased peroxisome abundance, higher peroxiso...
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| Veröffentlicht in: | Planta 2011-03, Vol.233 (3), p.507-521 |
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| creator | Valenzuela-Soto, José Humberto Iruegas-Bocardo, Fernanda Martínez-Gallardo, Norma Angélica Molina-Torres, Jorge Gómez-Lim, Miguel Ángel Délano-Frier, John Paul |
| description | Transgenic tobacco plants capable of over-expressing Xenopus PPARα (xPPARα), a transcription factor known to be required for peroxisome proliferation in animals, were recently generated. These plants (herewith referred to as PPAR-OE) were found to have increased peroxisome abundance, higher peroxisomal acyl-CoA oxidase and catalase activity and modified fatty acid metabolism. Further characterization of PPAR-OE plants revealed a higher susceptibility to virulent and a partial loss of resistance to avirulent Pseudomonas syringae pathogens, whereas the basal resistance response remained unaffected. Biochemical- and defense-related gene expression analyses showed that increased susceptibility to bacterial invasion coincided with the generalized reduction in H₂O₂ and salicylic acid (SA) levels observed within the first 24 h of bacterial contact. Decreased H₂O₂ levels were correlated with modified activity levels of catalase and other antioxidant enzymes. A correspondence between a rapid (within 1-24 hpi; ACCO and AOC) and sustained increase (up to 6 days pi; ACCO) in the expression levels of ethylene (ACCO) and jasmonic acid (AOC) biosynthetic genes and a higher susceptibility to virulent bacterial invasion was also observed in PPAR-OE plants. Conversely, no apparent differences in the short- and/or long-term expression levels of markers for the hypersensitive-response, oxidative burst and systemic-acquired resistance were observed between wild type and PPAR-OE plants. The results suggest that peroxisome proliferation could lead to increased susceptibility to bacterial pathogens in tobacco by altering the redox balance of the plant and the expression pattern of key defense signaling pathway genes. |
| doi_str_mv | 10.1007/s00425-010-1314-7 |
| format | Article |
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These plants (herewith referred to as PPAR-OE) were found to have increased peroxisome abundance, higher peroxisomal acyl-CoA oxidase and catalase activity and modified fatty acid metabolism. Further characterization of PPAR-OE plants revealed a higher susceptibility to virulent and a partial loss of resistance to avirulent Pseudomonas syringae pathogens, whereas the basal resistance response remained unaffected. Biochemical- and defense-related gene expression analyses showed that increased susceptibility to bacterial invasion coincided with the generalized reduction in H₂O₂ and salicylic acid (SA) levels observed within the first 24 h of bacterial contact. Decreased H₂O₂ levels were correlated with modified activity levels of catalase and other antioxidant enzymes. A correspondence between a rapid (within 1-24 hpi; ACCO and AOC) and sustained increase (up to 6 days pi; ACCO) in the expression levels of ethylene (ACCO) and jasmonic acid (AOC) biosynthetic genes and a higher susceptibility to virulent bacterial invasion was also observed in PPAR-OE plants. Conversely, no apparent differences in the short- and/or long-term expression levels of markers for the hypersensitive-response, oxidative burst and systemic-acquired resistance were observed between wild type and PPAR-OE plants. The results suggest that peroxisome proliferation could lead to increased susceptibility to bacterial pathogens in tobacco by altering the redox balance of the plant and the expression pattern of key defense signaling pathway genes.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-010-1314-7</identifier><identifier>PMID: 21104271</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Acyl-CoA Oxidase ; Agriculture ; Animals ; Ascorbate Peroxidases ; Bacterial plant pathogens ; Biological and medical sciences ; Biomarkers - metabolism ; Biomedical and Life Sciences ; Catalase - metabolism ; Cyclopentanes - analysis ; Disease Susceptibility ; Ecology ; Forestry ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Hydrogen ; Hydrogen peroxide ; Hydrogen Peroxide - analysis ; Infections ; Leaves ; Life Sciences ; Nicotiana - genetics ; Nicotiana - metabolism ; Nicotiana - microbiology ; Original Article ; Oxidoreductases - metabolism ; Oxylipins - analysis ; Pathogens ; Peroxidases - metabolism ; Peroxisome proliferation ; Peroxisome proliferation-associated receptor ; Peroxisomes ; Peroxisomes - metabolism ; Phytopathology. Animal pests. Plant and forest protection ; Plant cells ; Plant Diseases - genetics ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant Immunity ; Plant interaction ; Plant Leaves - metabolism ; Plant Leaves - microbiology ; Plant Sciences ; Plants ; Plants, Genetically Modified - metabolism ; Plants, Genetically Modified - microbiology ; PPAR alpha - genetics ; PPAR alpha - metabolism ; Pseudomonas syringae ; Pseudomonas syringae - pathogenicity ; Reactive oxygen species ; salicylic acid ; Salicylic Acid - analysis ; Superoxide Dismutase - metabolism ; Time Factors ; Tobacco ; Xenopus Proteins - genetics ; Xenopus Proteins - metabolism</subject><ispartof>Planta, 2011-03, Vol.233 (3), p.507-521</ispartof><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-9a046b316248dd2c3acb051cb5c48019dc7b41d8c2ab8ca15239905fdc70c9a03</citedby><cites>FETCH-LOGICAL-c446t-9a046b316248dd2c3acb051cb5c48019dc7b41d8c2ab8ca15239905fdc70c9a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23883846$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23883846$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23928461$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21104271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valenzuela-Soto, José Humberto</creatorcontrib><creatorcontrib>Iruegas-Bocardo, Fernanda</creatorcontrib><creatorcontrib>Martínez-Gallardo, Norma Angélica</creatorcontrib><creatorcontrib>Molina-Torres, Jorge</creatorcontrib><creatorcontrib>Gómez-Lim, Miguel Ángel</creatorcontrib><creatorcontrib>Délano-Frier, John Paul</creatorcontrib><title>Transformed tobacco (Nicotiana tabacum) plants over-expressing a peroxisome proliferator-activated receptor gene from Xenopus laevis (xPPARα) show increased susceptibility to infection by virulent Pseudomonas syringae pathogens</title><title>Planta</title><addtitle>Planta</addtitle><addtitle>Planta</addtitle><description>Transgenic tobacco plants capable of over-expressing Xenopus PPARα (xPPARα), a transcription factor known to be required for peroxisome proliferation in animals, were recently generated. These plants (herewith referred to as PPAR-OE) were found to have increased peroxisome abundance, higher peroxisomal acyl-CoA oxidase and catalase activity and modified fatty acid metabolism. Further characterization of PPAR-OE plants revealed a higher susceptibility to virulent and a partial loss of resistance to avirulent Pseudomonas syringae pathogens, whereas the basal resistance response remained unaffected. Biochemical- and defense-related gene expression analyses showed that increased susceptibility to bacterial invasion coincided with the generalized reduction in H₂O₂ and salicylic acid (SA) levels observed within the first 24 h of bacterial contact. Decreased H₂O₂ levels were correlated with modified activity levels of catalase and other antioxidant enzymes. A correspondence between a rapid (within 1-24 hpi; ACCO and AOC) and sustained increase (up to 6 days pi; ACCO) in the expression levels of ethylene (ACCO) and jasmonic acid (AOC) biosynthetic genes and a higher susceptibility to virulent bacterial invasion was also observed in PPAR-OE plants. Conversely, no apparent differences in the short- and/or long-term expression levels of markers for the hypersensitive-response, oxidative burst and systemic-acquired resistance were observed between wild type and PPAR-OE plants. The results suggest that peroxisome proliferation could lead to increased susceptibility to bacterial pathogens in tobacco by altering the redox balance of the plant and the expression pattern of key defense signaling pathway genes.</description><subject>Acyl-CoA Oxidase</subject><subject>Agriculture</subject><subject>Animals</subject><subject>Ascorbate Peroxidases</subject><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Catalase - metabolism</subject><subject>Cyclopentanes - analysis</subject><subject>Disease Susceptibility</subject><subject>Ecology</subject><subject>Forestry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Hydrogen</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Infections</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana - microbiology</subject><subject>Original Article</subject><subject>Oxidoreductases - metabolism</subject><subject>Oxylipins - analysis</subject><subject>Pathogens</subject><subject>Peroxidases - metabolism</subject><subject>Peroxisome proliferation</subject><subject>Peroxisome proliferation-associated receptor</subject><subject>Peroxisomes</subject><subject>Peroxisomes - metabolism</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant cells</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Immunity</subject><subject>Plant interaction</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - microbiology</subject><subject>Plant Sciences</subject><subject>Plants</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plants, Genetically Modified - microbiology</subject><subject>PPAR alpha - genetics</subject><subject>PPAR alpha - metabolism</subject><subject>Pseudomonas syringae</subject><subject>Pseudomonas syringae - pathogenicity</subject><subject>Reactive oxygen species</subject><subject>salicylic acid</subject><subject>Salicylic Acid - analysis</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Time Factors</subject><subject>Tobacco</subject><subject>Xenopus Proteins - genetics</subject><subject>Xenopus Proteins - metabolism</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UUtu1EAQtRCIhMABWAAtJKRkYeifx_YyivhJEYwgkdhZ5XZ70iPb7XS1h5ljcRFOwUGokQfCipXbVe-neknyVPDXgvP8DXKuZZZywVOhhE7ze8mx0EqmkuvifnLMOb15qbKj5BHimnNa5vnD5EgKQcxcHCe_rgIM2PrQ24ZFX4Mxnp1-csZHBwOwCDSa-jM2djBEZH5jQ2q3Y7CIblgxYKMNfuvQ95aNwXeutQGiDymY6DYQSTZYY0casZUdLGuD79k3O_hxQtaB3Thkp9vl8vzLzx9nDG_8d-YGEywgUXHCPdfVrnNxRwFp11pS9gOrd2zjwtTZIbIl2qnxvR8AGe4CJQOKA_HGkyc-Th600KF9cvieJNfv3l5dfEgvP7__eHF-mRqtFzEtgetFrcRC6qJppFFgap4JU2dGF1yUjclrLZrCSKgLAyKTqix51tKcGyKrk-TlrEuHuJ0sxmrtpzCQZVVkSqlC8oJAYgaZ4BGDbasxuB7CrhK82tdazbVWfP9PtVY5cZ4fhKeaivrL-NMjAV4dAIAGupZKNQ7vcKqUhV7scXLG4bg_kg13Cf_n_mwmrZFa_Ee0KBSp0v7FvG_BV7AKZHz9VXKh6GZa0eXUb9Fa1JI</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Valenzuela-Soto, José Humberto</creator><creator>Iruegas-Bocardo, Fernanda</creator><creator>Martínez-Gallardo, Norma Angélica</creator><creator>Molina-Torres, Jorge</creator><creator>Gómez-Lim, Miguel Ángel</creator><creator>Délano-Frier, John Paul</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20110301</creationdate><title>Transformed tobacco (Nicotiana tabacum) plants over-expressing a peroxisome proliferator-activated receptor gene from Xenopus laevis (xPPARα) show increased susceptibility to infection by virulent Pseudomonas syringae pathogens</title><author>Valenzuela-Soto, José Humberto ; Iruegas-Bocardo, Fernanda ; Martínez-Gallardo, Norma Angélica ; Molina-Torres, Jorge ; Gómez-Lim, Miguel Ángel ; Délano-Frier, John Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-9a046b316248dd2c3acb051cb5c48019dc7b41d8c2ab8ca15239905fdc70c9a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acyl-CoA Oxidase</topic><topic>Agriculture</topic><topic>Animals</topic><topic>Ascorbate Peroxidases</topic><topic>Bacterial plant pathogens</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Catalase - metabolism</topic><topic>Cyclopentanes - analysis</topic><topic>Disease Susceptibility</topic><topic>Ecology</topic><topic>Forestry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Hydrogen</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - analysis</topic><topic>Infections</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana - microbiology</topic><topic>Original Article</topic><topic>Oxidoreductases - metabolism</topic><topic>Oxylipins - analysis</topic><topic>Pathogens</topic><topic>Peroxidases - metabolism</topic><topic>Peroxisome proliferation</topic><topic>Peroxisome proliferation-associated receptor</topic><topic>Peroxisomes</topic><topic>Peroxisomes - metabolism</topic><topic>Phytopathology. Animal pests. 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pathogens</atitle><jtitle>Planta</jtitle><stitle>Planta</stitle><addtitle>Planta</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>233</volume><issue>3</issue><spage>507</spage><epage>521</epage><pages>507-521</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Transgenic tobacco plants capable of over-expressing Xenopus PPARα (xPPARα), a transcription factor known to be required for peroxisome proliferation in animals, were recently generated. These plants (herewith referred to as PPAR-OE) were found to have increased peroxisome abundance, higher peroxisomal acyl-CoA oxidase and catalase activity and modified fatty acid metabolism. Further characterization of PPAR-OE plants revealed a higher susceptibility to virulent and a partial loss of resistance to avirulent Pseudomonas syringae pathogens, whereas the basal resistance response remained unaffected. Biochemical- and defense-related gene expression analyses showed that increased susceptibility to bacterial invasion coincided with the generalized reduction in H₂O₂ and salicylic acid (SA) levels observed within the first 24 h of bacterial contact. Decreased H₂O₂ levels were correlated with modified activity levels of catalase and other antioxidant enzymes. A correspondence between a rapid (within 1-24 hpi; ACCO and AOC) and sustained increase (up to 6 days pi; ACCO) in the expression levels of ethylene (ACCO) and jasmonic acid (AOC) biosynthetic genes and a higher susceptibility to virulent bacterial invasion was also observed in PPAR-OE plants. Conversely, no apparent differences in the short- and/or long-term expression levels of markers for the hypersensitive-response, oxidative burst and systemic-acquired resistance were observed between wild type and PPAR-OE plants. The results suggest that peroxisome proliferation could lead to increased susceptibility to bacterial pathogens in tobacco by altering the redox balance of the plant and the expression pattern of key defense signaling pathway genes.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>21104271</pmid><doi>10.1007/s00425-010-1314-7</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 2011-03, Vol.233 (3), p.507-521 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_journals_853338208 |
| source | MEDLINE; SpringerLink Journals; JSTOR Archive Collection A-Z Listing |
| subjects | Acyl-CoA Oxidase Agriculture Animals Ascorbate Peroxidases Bacterial plant pathogens Biological and medical sciences Biomarkers - metabolism Biomedical and Life Sciences Catalase - metabolism Cyclopentanes - analysis Disease Susceptibility Ecology Forestry Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Hydrogen Hydrogen peroxide Hydrogen Peroxide - analysis Infections Leaves Life Sciences Nicotiana - genetics Nicotiana - metabolism Nicotiana - microbiology Original Article Oxidoreductases - metabolism Oxylipins - analysis Pathogens Peroxidases - metabolism Peroxisome proliferation Peroxisome proliferation-associated receptor Peroxisomes Peroxisomes - metabolism Phytopathology. Animal pests. Plant and forest protection Plant cells Plant Diseases - genetics Plant Diseases - immunology Plant Diseases - microbiology Plant Immunity Plant interaction Plant Leaves - metabolism Plant Leaves - microbiology Plant Sciences Plants Plants, Genetically Modified - metabolism Plants, Genetically Modified - microbiology PPAR alpha - genetics PPAR alpha - metabolism Pseudomonas syringae Pseudomonas syringae - pathogenicity Reactive oxygen species salicylic acid Salicylic Acid - analysis Superoxide Dismutase - metabolism Time Factors Tobacco Xenopus Proteins - genetics Xenopus Proteins - metabolism |
| title | Transformed tobacco (Nicotiana tabacum) plants over-expressing a peroxisome proliferator-activated receptor gene from Xenopus laevis (xPPARα) show increased susceptibility to infection by virulent Pseudomonas syringae pathogens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T07%3A50%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transformed%20tobacco%20(Nicotiana%20tabacum)%20plants%20over-expressing%20a%20peroxisome%20proliferator-activated%20receptor%20gene%20from%20Xenopus%20laevis%20(xPPAR%CE%B1)%20show%20increased%20susceptibility%20to%20infection%20by%20virulent%20Pseudomonas%20syringae%20pathogens&rft.jtitle=Planta&rft.au=Valenzuela-Soto,%20Jos%C3%A9%20Humberto&rft.date=2011-03-01&rft.volume=233&rft.issue=3&rft.spage=507&rft.epage=521&rft.pages=507-521&rft.issn=0032-0935&rft.eissn=1432-2048&rft.coden=PLANAB&rft_id=info:doi/10.1007/s00425-010-1314-7&rft_dat=%3Cjstor_proqu%3E23883846%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=853338208&rft_id=info:pmid/21104271&rft_jstor_id=23883846&rfr_iscdi=true |