Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1[alpha] mRNA: a role of [Beta]^sub 2^-adrenergic receptor activation
There are three isoforms of peroxisome proliferator-activated receptor-... coactivator 1α (PGC-1α) mRNA, which promotes mitochondrial biogenesis in skeletal muscles. Compared with PGC-1α-a mRNA, PGC-1α-b or PGC-1α-c mRNA is transcribed by a different exon 1 of the PGC-1α gene. In this study, effects...
Gespeichert in:
Veröffentlicht in: | American journal of physiology: endocrinology and metabolism 2011-02, Vol.300 (2), p.E341 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 2 |
container_start_page | E341 |
container_title | American journal of physiology: endocrinology and metabolism |
container_volume | 300 |
creator | Tadaishi, Miki Miura, Shinji Kai, Yuko Kawasaki, Emi Koshinaka, Keiichi Kawanaka, Kentaro Nagata, Junichi Oishi, Yuichi Ezaki, Osamu |
description | There are three isoforms of peroxisome proliferator-activated receptor-... coactivator 1α (PGC-1α) mRNA, which promotes mitochondrial biogenesis in skeletal muscles. Compared with PGC-1α-a mRNA, PGC-1α-b or PGC-1α-c mRNA is transcribed by a different exon 1 of the PGC-1α gene. In this study, effects of exercise intensity and 5-aminoimidazole-4-carboxamide-1β-d-ribofuranoside (AICAR) on isoform-specific expressions of PGC-1α were investigated. All isoforms were increased in proportion to exercise intensity of treadmill running (10-30 m/min for 30 min). Preinjection of β...-adrenergic receptor (AR) antagonist (ICI 118551) inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs, but not the increase in PGC-1α-a mRNA, in response to high-intensity exercise. Although high-intensity exercise activated α2-AMP-activated protein kinase (α2-AMPK) in skeletal muscles, inactivation of α2-AMPK activity did not affect high-intensity exercise-induced mRNA expression of all PGC-1α isoforms, suggesting that activation of α2-AMPK is not mandatory for an increase in PGC-1α mRNA by high-intensity exercise. A single injection in mice of AICAR, an AMPK activator, increased mRNAs of all PGC-1α isoforms. AICAR increased blood catecholamine concentrations, and preinjection of β...-AR antagonist inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs but not the increase in PGC-1α-a mRNA. Direct exposure of epitrochlearis muscle to AICAR increased PGC-1α-a but not the -b isoform. These data indicate that exercise-induced PGC-1α expression was dependent on the intensity of exercise. Exercise or AICAR injection increased PGC-1α-b and PGC-1α-c mRNAs via β...-AR activation, whereas high-intensity exercise increased PGC-1α-a expression by a multiple mechanism in which α2-AMPK is one of the signaling pathways. (ProQuest: ... denotes formulae/symbols omitted.) |
format | Article |
fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_850923461</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2262881321</sourcerecordid><originalsourceid>FETCH-proquest_journals_8509234613</originalsourceid><addsrcrecordid>eNqNjU1OwzAUhC0EEuHnDk_sLcVJDA27EBXoBqGKXdVWxn0Gl8Q2fg6CK3FKjMQBWI1GM9_MASuErCoupJSHrChFW3Mxa9pjdkK0L8vySjZVwb7nxqBO4A3gJ0ZtCcG6hI5s-gLldtAt-m4J3oElb3wcOQXU1lidgRCRyHpHv_w4ResQ6A0HTGrInvSA8HjXc7FSQ3hVaxiXD901KIg-J5lZ3eTqekPTM1QbrnYRHcaXvB1RY0g-gtLJfqiUT87YkVED4fmfnrKL2_lTf89D9O8TUtru_RRdjrYzWbZV3VyK-l-lH6rMX-o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>850923461</pqid></control><display><type>article</type><title>Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1[alpha] mRNA: a role of [Beta]^sub 2^-adrenergic receptor activation</title><source>American Physiological Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Tadaishi, Miki ; Miura, Shinji ; Kai, Yuko ; Kawasaki, Emi ; Koshinaka, Keiichi ; Kawanaka, Kentaro ; Nagata, Junichi ; Oishi, Yuichi ; Ezaki, Osamu</creator><creatorcontrib>Tadaishi, Miki ; Miura, Shinji ; Kai, Yuko ; Kawasaki, Emi ; Koshinaka, Keiichi ; Kawanaka, Kentaro ; Nagata, Junichi ; Oishi, Yuichi ; Ezaki, Osamu</creatorcontrib><description>There are three isoforms of peroxisome proliferator-activated receptor-... coactivator 1α (PGC-1α) mRNA, which promotes mitochondrial biogenesis in skeletal muscles. Compared with PGC-1α-a mRNA, PGC-1α-b or PGC-1α-c mRNA is transcribed by a different exon 1 of the PGC-1α gene. In this study, effects of exercise intensity and 5-aminoimidazole-4-carboxamide-1β-d-ribofuranoside (AICAR) on isoform-specific expressions of PGC-1α were investigated. All isoforms were increased in proportion to exercise intensity of treadmill running (10-30 m/min for 30 min). Preinjection of β...-adrenergic receptor (AR) antagonist (ICI 118551) inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs, but not the increase in PGC-1α-a mRNA, in response to high-intensity exercise. Although high-intensity exercise activated α2-AMP-activated protein kinase (α2-AMPK) in skeletal muscles, inactivation of α2-AMPK activity did not affect high-intensity exercise-induced mRNA expression of all PGC-1α isoforms, suggesting that activation of α2-AMPK is not mandatory for an increase in PGC-1α mRNA by high-intensity exercise. A single injection in mice of AICAR, an AMPK activator, increased mRNAs of all PGC-1α isoforms. AICAR increased blood catecholamine concentrations, and preinjection of β...-AR antagonist inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs but not the increase in PGC-1α-a mRNA. Direct exposure of epitrochlearis muscle to AICAR increased PGC-1α-a but not the -b isoform. These data indicate that exercise-induced PGC-1α expression was dependent on the intensity of exercise. Exercise or AICAR injection increased PGC-1α-b and PGC-1α-c mRNAs via β...-AR activation, whereas high-intensity exercise increased PGC-1α-a expression by a multiple mechanism in which α2-AMPK is one of the signaling pathways. (ProQuest: ... denotes formulae/symbols omitted.)</description><identifier>ISSN: 0193-1849</identifier><identifier>EISSN: 1522-1555</identifier><identifier>CODEN: AJPMD9</identifier><language>eng</language><publisher>Bethesda: American Physiological Society</publisher><subject>Biosynthesis ; Exercise ; Genes ; Kinases ; Mitochondria ; Musculoskeletal system ; Ribonucleic acid ; RNA</subject><ispartof>American journal of physiology: endocrinology and metabolism, 2011-02, Vol.300 (2), p.E341</ispartof><rights>Copyright American Physiological Society Feb 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Tadaishi, Miki</creatorcontrib><creatorcontrib>Miura, Shinji</creatorcontrib><creatorcontrib>Kai, Yuko</creatorcontrib><creatorcontrib>Kawasaki, Emi</creatorcontrib><creatorcontrib>Koshinaka, Keiichi</creatorcontrib><creatorcontrib>Kawanaka, Kentaro</creatorcontrib><creatorcontrib>Nagata, Junichi</creatorcontrib><creatorcontrib>Oishi, Yuichi</creatorcontrib><creatorcontrib>Ezaki, Osamu</creatorcontrib><title>Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1[alpha] mRNA: a role of [Beta]^sub 2^-adrenergic receptor activation</title><title>American journal of physiology: endocrinology and metabolism</title><description>There are three isoforms of peroxisome proliferator-activated receptor-... coactivator 1α (PGC-1α) mRNA, which promotes mitochondrial biogenesis in skeletal muscles. Compared with PGC-1α-a mRNA, PGC-1α-b or PGC-1α-c mRNA is transcribed by a different exon 1 of the PGC-1α gene. In this study, effects of exercise intensity and 5-aminoimidazole-4-carboxamide-1β-d-ribofuranoside (AICAR) on isoform-specific expressions of PGC-1α were investigated. All isoforms were increased in proportion to exercise intensity of treadmill running (10-30 m/min for 30 min). Preinjection of β...-adrenergic receptor (AR) antagonist (ICI 118551) inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs, but not the increase in PGC-1α-a mRNA, in response to high-intensity exercise. Although high-intensity exercise activated α2-AMP-activated protein kinase (α2-AMPK) in skeletal muscles, inactivation of α2-AMPK activity did not affect high-intensity exercise-induced mRNA expression of all PGC-1α isoforms, suggesting that activation of α2-AMPK is not mandatory for an increase in PGC-1α mRNA by high-intensity exercise. A single injection in mice of AICAR, an AMPK activator, increased mRNAs of all PGC-1α isoforms. AICAR increased blood catecholamine concentrations, and preinjection of β...-AR antagonist inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs but not the increase in PGC-1α-a mRNA. Direct exposure of epitrochlearis muscle to AICAR increased PGC-1α-a but not the -b isoform. These data indicate that exercise-induced PGC-1α expression was dependent on the intensity of exercise. Exercise or AICAR injection increased PGC-1α-b and PGC-1α-c mRNAs via β...-AR activation, whereas high-intensity exercise increased PGC-1α-a expression by a multiple mechanism in which α2-AMPK is one of the signaling pathways. (ProQuest: ... denotes formulae/symbols omitted.)</description><subject>Biosynthesis</subject><subject>Exercise</subject><subject>Genes</subject><subject>Kinases</subject><subject>Mitochondria</subject><subject>Musculoskeletal system</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>0193-1849</issn><issn>1522-1555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqNjU1OwzAUhC0EEuHnDk_sLcVJDA27EBXoBqGKXdVWxn0Gl8Q2fg6CK3FKjMQBWI1GM9_MASuErCoupJSHrChFW3Mxa9pjdkK0L8vySjZVwb7nxqBO4A3gJ0ZtCcG6hI5s-gLldtAt-m4J3oElb3wcOQXU1lidgRCRyHpHv_w4ResQ6A0HTGrInvSA8HjXc7FSQ3hVaxiXD901KIg-J5lZ3eTqekPTM1QbrnYRHcaXvB1RY0g-gtLJfqiUT87YkVED4fmfnrKL2_lTf89D9O8TUtru_RRdjrYzWbZV3VyK-l-lH6rMX-o</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Tadaishi, Miki</creator><creator>Miura, Shinji</creator><creator>Kai, Yuko</creator><creator>Kawasaki, Emi</creator><creator>Koshinaka, Keiichi</creator><creator>Kawanaka, Kentaro</creator><creator>Nagata, Junichi</creator><creator>Oishi, Yuichi</creator><creator>Ezaki, Osamu</creator><general>American Physiological Society</general><scope>7QP</scope><scope>7TS</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20110201</creationdate><title>Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1[alpha] mRNA: a role of [Beta]^sub 2^-adrenergic receptor activation</title><author>Tadaishi, Miki ; Miura, Shinji ; Kai, Yuko ; Kawasaki, Emi ; Koshinaka, Keiichi ; Kawanaka, Kentaro ; Nagata, Junichi ; Oishi, Yuichi ; Ezaki, Osamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_8509234613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biosynthesis</topic><topic>Exercise</topic><topic>Genes</topic><topic>Kinases</topic><topic>Mitochondria</topic><topic>Musculoskeletal system</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tadaishi, Miki</creatorcontrib><creatorcontrib>Miura, Shinji</creatorcontrib><creatorcontrib>Kai, Yuko</creatorcontrib><creatorcontrib>Kawasaki, Emi</creatorcontrib><creatorcontrib>Koshinaka, Keiichi</creatorcontrib><creatorcontrib>Kawanaka, Kentaro</creatorcontrib><creatorcontrib>Nagata, Junichi</creatorcontrib><creatorcontrib>Oishi, Yuichi</creatorcontrib><creatorcontrib>Ezaki, Osamu</creatorcontrib><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>American journal of physiology: endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tadaishi, Miki</au><au>Miura, Shinji</au><au>Kai, Yuko</au><au>Kawasaki, Emi</au><au>Koshinaka, Keiichi</au><au>Kawanaka, Kentaro</au><au>Nagata, Junichi</au><au>Oishi, Yuichi</au><au>Ezaki, Osamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1[alpha] mRNA: a role of [Beta]^sub 2^-adrenergic receptor activation</atitle><jtitle>American journal of physiology: endocrinology and metabolism</jtitle><date>2011-02-01</date><risdate>2011</risdate><volume>300</volume><issue>2</issue><spage>E341</spage><pages>E341-</pages><issn>0193-1849</issn><eissn>1522-1555</eissn><coden>AJPMD9</coden><abstract>There are three isoforms of peroxisome proliferator-activated receptor-... coactivator 1α (PGC-1α) mRNA, which promotes mitochondrial biogenesis in skeletal muscles. Compared with PGC-1α-a mRNA, PGC-1α-b or PGC-1α-c mRNA is transcribed by a different exon 1 of the PGC-1α gene. In this study, effects of exercise intensity and 5-aminoimidazole-4-carboxamide-1β-d-ribofuranoside (AICAR) on isoform-specific expressions of PGC-1α were investigated. All isoforms were increased in proportion to exercise intensity of treadmill running (10-30 m/min for 30 min). Preinjection of β...-adrenergic receptor (AR) antagonist (ICI 118551) inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs, but not the increase in PGC-1α-a mRNA, in response to high-intensity exercise. Although high-intensity exercise activated α2-AMP-activated protein kinase (α2-AMPK) in skeletal muscles, inactivation of α2-AMPK activity did not affect high-intensity exercise-induced mRNA expression of all PGC-1α isoforms, suggesting that activation of α2-AMPK is not mandatory for an increase in PGC-1α mRNA by high-intensity exercise. A single injection in mice of AICAR, an AMPK activator, increased mRNAs of all PGC-1α isoforms. AICAR increased blood catecholamine concentrations, and preinjection of β...-AR antagonist inhibited the increase in PGC-1α-b and PGC-1α-c mRNAs but not the increase in PGC-1α-a mRNA. Direct exposure of epitrochlearis muscle to AICAR increased PGC-1α-a but not the -b isoform. These data indicate that exercise-induced PGC-1α expression was dependent on the intensity of exercise. Exercise or AICAR injection increased PGC-1α-b and PGC-1α-c mRNAs via β...-AR activation, whereas high-intensity exercise increased PGC-1α-a expression by a multiple mechanism in which α2-AMPK is one of the signaling pathways. (ProQuest: ... denotes formulae/symbols omitted.)</abstract><cop>Bethesda</cop><pub>American Physiological Society</pub></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0193-1849 |
ispartof | American journal of physiology: endocrinology and metabolism, 2011-02, Vol.300 (2), p.E341 |
issn | 0193-1849 1522-1555 |
language | eng |
recordid | cdi_proquest_journals_850923461 |
source | American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | Biosynthesis Exercise Genes Kinases Mitochondria Musculoskeletal system Ribonucleic acid RNA |
title | Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1[alpha] mRNA: a role of [Beta]^sub 2^-adrenergic receptor activation |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A07%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20exercise%20intensity%20and%20AICAR%20on%20isoform-specific%20expressions%20of%20murine%20skeletal%20muscle%20PGC-1%5Balpha%5D%20mRNA:%20a%20role%20of%20%5BBeta%5D%5Esub%202%5E-adrenergic%20receptor%20activation&rft.jtitle=American%20journal%20of%20physiology:%20endocrinology%20and%20metabolism&rft.au=Tadaishi,%20Miki&rft.date=2011-02-01&rft.volume=300&rft.issue=2&rft.spage=E341&rft.pages=E341-&rft.issn=0193-1849&rft.eissn=1522-1555&rft.coden=AJPMD9&rft_id=info:doi/&rft_dat=%3Cproquest%3E2262881321%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=850923461&rft_id=info:pmid/&rfr_iscdi=true |