Disruption of ten protease genes in the filamentous fungus Aspergillus oryzae highly improves production of heterologous proteins

Proteolytic degradation by secreted proteases into the culture medium is one of the significant problems to be solved in heterologous protein production by filamentous fungi including Aspergillus oryzae. Double (tppA, and pepE) and quintuple (tppA, pepE, nptB, dppIV, and dppV) disruption of protease...

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Veröffentlicht in:	Applied microbiology and biotechnology 2011-02, Vol.89 (3), p.747-759
Hauptverfasser:	Yoon, Jaewoo, Maruyama, Jun-ichi, Kitamoto, Katsuhiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied Genetics and Molecular Biotechnology Aspergillus oryzae Aspergillus oryzae - enzymology Aspergillus oryzae - genetics Biological and medical sciences Biomedical and Life Sciences Biotechnology Biotechnology - methods Bovine chymosin Decuple protease gene disruption DNA microarray analysis Enzymes Fundamental and applied biological sciences. Psychology Fungi Gene expression Gene Knockout Techniques Genetic engineering Heterologous protein production Human lysozyme Life Sciences Microbial Genetics and Genomics Microbiology Peptide Hydrolases - genetics Peptide Hydrolases - metabolism Plasmids Protease inhibitors Proteins Recombinant Proteins - biosynthesis Studies Yeast
Online-Zugang:	Volltext
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