Tissue microarray technology: validation in colorectal carcinoma and analysis of p53, hMLH1, and hMSH2 immunohistochemical expression
Tissue microarray technology enables the analysis of hundreds of specimens by arranging numerous 0.6-mm tissue core biopsy specimens into a single paraffin block. Validation studies are necessary to evaluate the representativeness of small disks taken from the original tissue. We validated the tissu...
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| Veröffentlicht in: | Virchows Archiv : an international journal of pathology 2003-08, Vol.443 (2), p.115-121 |
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| container_title | Virchows Archiv : an international journal of pathology |
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| creator | Jourdan, Florence Sebbagh, Nicole Comperat, Eva Mourra, Najat Flahault, Antoine Olschwang, Sylviane Duval, Alex Hamelin, Richard Flejou, Jean-François |
| description | Tissue microarray technology enables the analysis of hundreds of specimens by arranging numerous 0.6-mm tissue core biopsy specimens into a single paraffin block. Validation studies are necessary to evaluate the representativeness of small disks taken from the original tissue. We validated the tissue microarray technology in colorectal carcinoma by analyzing the immunohistochemical expression of proteins involved in the two main pathways of colorectal carcinogenesis: p53 protein for loss of heterozygosity tumors, hMLH1 and hMSH2 proteins for microsatellite instability (MSI) tumors. We compared in 30 colorectal carcinomas (15 MSI(-) and 15 MSI(+)), 8 microarrays disks, and the whole section of the block from which they were derived. Tumoral tissue was present in 95.7% of the microarray disks. The analysis of three disks per case was comparable to the analysis of the whole section in 99.6% (p53), 98.8% (hMLH1), and 99.2% (hMSH2) of cases. In the second part we applied the tissue microarray technology to 263 consecutive cases of colorectal carcinoma, sampled by three cores. We showed that 48.5% overexpressed p53 and 8.7% lost hMLH1 or hMSH2. Tissue microarray technology, validated in colorectal carcinoma, appears as a useful research tool for rapid analysis of the clinical interest of molecular alterations. |
| doi_str_mv | 10.1007/s00428-003-0833-z |
| format | Article |
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Validation studies are necessary to evaluate the representativeness of small disks taken from the original tissue. We validated the tissue microarray technology in colorectal carcinoma by analyzing the immunohistochemical expression of proteins involved in the two main pathways of colorectal carcinogenesis: p53 protein for loss of heterozygosity tumors, hMLH1 and hMSH2 proteins for microsatellite instability (MSI) tumors. We compared in 30 colorectal carcinomas (15 MSI(-) and 15 MSI(+)), 8 microarrays disks, and the whole section of the block from which they were derived. Tumoral tissue was present in 95.7% of the microarray disks. The analysis of three disks per case was comparable to the analysis of the whole section in 99.6% (p53), 98.8% (hMLH1), and 99.2% (hMSH2) of cases. In the second part we applied the tissue microarray technology to 263 consecutive cases of colorectal carcinoma, sampled by three cores. We showed that 48.5% overexpressed p53 and 8.7% lost hMLH1 or hMSH2. Tissue microarray technology, validated in colorectal carcinoma, appears as a useful research tool for rapid analysis of the clinical interest of molecular alterations.</description><identifier>ISSN: 0945-6317</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/s00428-003-0833-z</identifier><identifier>PMID: 12802583</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Adaptor Proteins, Signal Transducing ; Adenocarcinoma - chemistry ; Adenocarcinoma - pathology ; Biomarkers, Tumor - analysis ; Carcinogenesis ; Carrier Proteins ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - chemistry ; Colorectal Neoplasms - pathology ; DNA-Binding Proteins ; Female ; Genes ; Histocytological Preparation Techniques ; Humans ; Immunoenzyme Techniques ; Male ; MutL Protein Homolog 1 ; MutS Homolog 2 Protein ; Neoplasm Proteins - analysis ; Nuclear Proteins ; Proto-Oncogene Proteins - analysis ; Tissues ; Tumor Suppressor Protein p53 - analysis</subject><ispartof>Virchows Archiv : an international journal of pathology, 2003-08, Vol.443 (2), p.115-121</ispartof><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-8583fd0b08be31e975266ed19d51899933f3f1c1da7bff8013c7e53266e932553</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12802583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jourdan, Florence</creatorcontrib><creatorcontrib>Sebbagh, Nicole</creatorcontrib><creatorcontrib>Comperat, Eva</creatorcontrib><creatorcontrib>Mourra, Najat</creatorcontrib><creatorcontrib>Flahault, Antoine</creatorcontrib><creatorcontrib>Olschwang, Sylviane</creatorcontrib><creatorcontrib>Duval, Alex</creatorcontrib><creatorcontrib>Hamelin, Richard</creatorcontrib><creatorcontrib>Flejou, Jean-François</creatorcontrib><title>Tissue microarray technology: validation in colorectal carcinoma and analysis of p53, hMLH1, and hMSH2 immunohistochemical expression</title><title>Virchows Archiv : an international journal of pathology</title><addtitle>Virchows Arch</addtitle><description>Tissue microarray technology enables the analysis of hundreds of specimens by arranging numerous 0.6-mm tissue core biopsy specimens into a single paraffin block. Validation studies are necessary to evaluate the representativeness of small disks taken from the original tissue. We validated the tissue microarray technology in colorectal carcinoma by analyzing the immunohistochemical expression of proteins involved in the two main pathways of colorectal carcinogenesis: p53 protein for loss of heterozygosity tumors, hMLH1 and hMSH2 proteins for microsatellite instability (MSI) tumors. We compared in 30 colorectal carcinomas (15 MSI(-) and 15 MSI(+)), 8 microarrays disks, and the whole section of the block from which they were derived. Tumoral tissue was present in 95.7% of the microarray disks. The analysis of three disks per case was comparable to the analysis of the whole section in 99.6% (p53), 98.8% (hMLH1), and 99.2% (hMSH2) of cases. In the second part we applied the tissue microarray technology to 263 consecutive cases of colorectal carcinoma, sampled by three cores. We showed that 48.5% overexpressed p53 and 8.7% lost hMLH1 or hMSH2. Tissue microarray technology, validated in colorectal carcinoma, appears as a useful research tool for rapid analysis of the clinical interest of molecular alterations.</description><subject>Adaptor Proteins, Signal Transducing</subject><subject>Adenocarcinoma - chemistry</subject><subject>Adenocarcinoma - pathology</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Carcinogenesis</subject><subject>Carrier Proteins</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - chemistry</subject><subject>Colorectal Neoplasms - pathology</subject><subject>DNA-Binding Proteins</subject><subject>Female</subject><subject>Genes</subject><subject>Histocytological Preparation Techniques</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Male</subject><subject>MutL Protein Homolog 1</subject><subject>MutS Homolog 2 Protein</subject><subject>Neoplasm Proteins - analysis</subject><subject>Nuclear 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Validation studies are necessary to evaluate the representativeness of small disks taken from the original tissue. We validated the tissue microarray technology in colorectal carcinoma by analyzing the immunohistochemical expression of proteins involved in the two main pathways of colorectal carcinogenesis: p53 protein for loss of heterozygosity tumors, hMLH1 and hMSH2 proteins for microsatellite instability (MSI) tumors. We compared in 30 colorectal carcinomas (15 MSI(-) and 15 MSI(+)), 8 microarrays disks, and the whole section of the block from which they were derived. Tumoral tissue was present in 95.7% of the microarray disks. The analysis of three disks per case was comparable to the analysis of the whole section in 99.6% (p53), 98.8% (hMLH1), and 99.2% (hMSH2) of cases. In the second part we applied the tissue microarray technology to 263 consecutive cases of colorectal carcinoma, sampled by three cores. We showed that 48.5% overexpressed p53 and 8.7% lost hMLH1 or hMSH2. 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| ispartof | Virchows Archiv : an international journal of pathology, 2003-08, Vol.443 (2), p.115-121 |
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| subjects | Adaptor Proteins, Signal Transducing Adenocarcinoma - chemistry Adenocarcinoma - pathology Biomarkers, Tumor - analysis Carcinogenesis Carrier Proteins Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - chemistry Colorectal Neoplasms - pathology DNA-Binding Proteins Female Genes Histocytological Preparation Techniques Humans Immunoenzyme Techniques Male MutL Protein Homolog 1 MutS Homolog 2 Protein Neoplasm Proteins - analysis Nuclear Proteins Proto-Oncogene Proteins - analysis Tissues Tumor Suppressor Protein p53 - analysis |
| title | Tissue microarray technology: validation in colorectal carcinoma and analysis of p53, hMLH1, and hMSH2 immunohistochemical expression |
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