Effects of colchicine and cold duration pretreatments on androgenesis responses of tomato (Lycopersicon esculentum Mill) via anther culture
The aim of the present study was to optimize the in vitro chromosome-doubling procedure by colchicine pretreatment in tomato anther culture. Anthers of 1.7–2.0 mm long were isolated at prophase-metaphase I and pretreated by different concentrations of colchicine (0, 250, 500 and 750 mg/l) at 4°C for...
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Veröffentlicht in: | Russian agricultural sciences 2010-10, Vol.36 (5), p.338-341 |
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Sprache: | eng |
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