Emergence and Characterization of Foodborne Methicillin-Resistant Staphylococcus aureus in Korea
A total of 165 Staphylococcus aureus strains, isolated from different food samples between 2003 and 2006, were tested for antimicrobial susceptibility. The mecA-positive methicillin-resistant S. aureus (MRSA) strains were further characterized by testing for various virulence genes and by molecular...
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| description | A total of 165 Staphylococcus aureus strains, isolated from different food samples between 2003 and 2006, were tested for antimicrobial susceptibility. The mecA-positive methicillin-resistant S. aureus (MRSA) strains were further characterized by testing for various virulence genes and by molecular typing with multilocus sequence typing and pulsed-field gel electrophoresis. Of the 165 S. aureus isolates, 150 strains (90.9%) were resistant to at least one antibiotic while no strain was resistant to vancomycin. Four strains were resistant to both oxacillin and cefoxitin and were mecA positive. The mecA-positive MRSA strains were isolated from raw meat and fish samples (two beef samples and two fish samples) and were resistant to β-lactam antibiotics. Based on multilocus sequence typing analysis, the isolates were assigned to sequence type 1 (ST1), ST72, and an undetermined ST (ST72 slv). All four MRSA isolates were shown to be enterotoxigenic. The ST1 MRSA isolate harbored the sea-seh gene combination and the ST72 and ST72 slv MRSA strains harbored the seg-sei and the sea-seg-sei gene combinations, respectively. However, none of the MRSA isolates had the genes for Panton-Valentine leukocidin, toxic shock syndrome toxin 1, and exfoliative toxins. The pulsed-field gel electrophoresis patterns of the ST72 isolates in our study were highly similar, even though they were isolated from food samples in different years and from different regions of Korea. |
| doi_str_mv | 10.4315/0362-028X-73.12.2285 |
| format | Article |
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The mecA-positive methicillin-resistant S. aureus (MRSA) strains were further characterized by testing for various virulence genes and by molecular typing with multilocus sequence typing and pulsed-field gel electrophoresis. Of the 165 S. aureus isolates, 150 strains (90.9%) were resistant to at least one antibiotic while no strain was resistant to vancomycin. Four strains were resistant to both oxacillin and cefoxitin and were mecA positive. The mecA-positive MRSA strains were isolated from raw meat and fish samples (two beef samples and two fish samples) and were resistant to β-lactam antibiotics. Based on multilocus sequence typing analysis, the isolates were assigned to sequence type 1 (ST1), ST72, and an undetermined ST (ST72 slv). All four MRSA isolates were shown to be enterotoxigenic. The ST1 MRSA isolate harbored the sea-seh gene combination and the ST72 and ST72 slv MRSA strains harbored the seg-sei and the sea-seg-sei gene combinations, respectively. However, none of the MRSA isolates had the genes for Panton-Valentine leukocidin, toxic shock syndrome toxin 1, and exfoliative toxins. The pulsed-field gel electrophoresis patterns of the ST72 isolates in our study were highly similar, even though they were isolated from food samples in different years and from different regions of Korea.</description><identifier>ISSN: 0362-028X</identifier><identifier>EISSN: 1944-9097</identifier><identifier>DOI: 10.4315/0362-028X-73.12.2285</identifier><identifier>PMID: 21219750</identifier><identifier>CODEN: JFPRDR</identifier><language>eng</language><publisher>Des Moines, IA: International Association for Food Protection</publisher><subject>Annealing ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; Biological and medical sciences ; Colony Count, Microbial ; Consumer Product Safety ; DNA, Bacterial - analysis ; Drug resistance ; Electrophoresis ; Enterotoxins - genetics ; Fish Products - microbiology ; Food contamination & poisoning ; Food Contamination - analysis ; Food industries ; Food Microbiology ; Food safety ; Fundamental and applied biological sciences. Psychology ; Genes ; Genotype ; Humans ; Korea ; Laboratories ; Meat Products - microbiology ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - pathogenicity ; Microbial Sensitivity Tests ; Molecular Epidemiology ; Pathogens ; Penicillin ; Prevalence ; Staphylococcal Food Poisoning - epidemiology ; Staphylococcal Food Poisoning - microbiology ; Staphylococcus infections ; Toxins ; Virulence</subject><ispartof>Journal of food protection, 2010-12, Vol.73 (12), p.2285-2290</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright International Association for Food Protection Dec 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23624545$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21219750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHAE HONG RHEE</creatorcontrib><creatorcontrib>WOO, Gun-Jo</creatorcontrib><title>Emergence and Characterization of Foodborne Methicillin-Resistant Staphylococcus aureus in Korea</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>A total of 165 Staphylococcus aureus strains, isolated from different food samples between 2003 and 2006, were tested for antimicrobial susceptibility. The mecA-positive methicillin-resistant S. aureus (MRSA) strains were further characterized by testing for various virulence genes and by molecular typing with multilocus sequence typing and pulsed-field gel electrophoresis. Of the 165 S. aureus isolates, 150 strains (90.9%) were resistant to at least one antibiotic while no strain was resistant to vancomycin. Four strains were resistant to both oxacillin and cefoxitin and were mecA positive. The mecA-positive MRSA strains were isolated from raw meat and fish samples (two beef samples and two fish samples) and were resistant to β-lactam antibiotics. Based on multilocus sequence typing analysis, the isolates were assigned to sequence type 1 (ST1), ST72, and an undetermined ST (ST72 slv). All four MRSA isolates were shown to be enterotoxigenic. The ST1 MRSA isolate harbored the sea-seh gene combination and the ST72 and ST72 slv MRSA strains harbored the seg-sei and the sea-seg-sei gene combinations, respectively. However, none of the MRSA isolates had the genes for Panton-Valentine leukocidin, toxic shock syndrome toxin 1, and exfoliative toxins. The pulsed-field gel electrophoresis patterns of the ST72 isolates in our study were highly similar, even though they were isolated from food samples in different years and from different regions of Korea.</description><subject>Annealing</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Consumer Product Safety</subject><subject>DNA, Bacterial - analysis</subject><subject>Drug resistance</subject><subject>Electrophoresis</subject><subject>Enterotoxins - genetics</subject><subject>Fish Products - microbiology</subject><subject>Food contamination & poisoning</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Food safety</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genotype</subject><subject>Humans</subject><subject>Korea</subject><subject>Laboratories</subject><subject>Meat Products - microbiology</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - pathogenicity</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Epidemiology</subject><subject>Pathogens</subject><subject>Penicillin</subject><subject>Prevalence</subject><subject>Staphylococcal Food Poisoning - epidemiology</subject><subject>Staphylococcal Food Poisoning - microbiology</subject><subject>Staphylococcus infections</subject><subject>Toxins</subject><subject>Virulence</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpF0FFLwzAQB_AgipvTbyASBB87k0vTNo8yNhUngvPBt3pLUxfpkpq0D_PTW3Dq08Hdj7vjT8g5Z9NUcHnNRAYJg-I1ycWUwxSgkAdkzFWaJoqp_JCM_8iInMT4wRgDBdkxGQEHrnLJxuRtvjXh3ThtKLqKzjYYUHcm2C_srHfU13ThfbX2wRn6aLqN1bZprEueTbSxQ9fRVYftZtd47bXuI8U-mKFYRx98MHhKjmpsojnb1wlZLeYvs7tk-XR7P7tZJi0I2SUGiiwr0hqUEtzg0KsRVDX8mAHULAeOzGRS1iBRDVMmBDCtWJFCLlBMyOXP1jb4z97ErvzwfXDDwbLgaZFBzosBXexRv96aqmyD3WLYlb9pDOBqDzBqbOqATtv474Y4U5lK8Q0czm5D</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>CHAE HONG RHEE</creator><creator>WOO, Gun-Jo</creator><general>International Association for Food Protection</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7RQ</scope><scope>7WY</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>883</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0F</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PATMY</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope></search><sort><creationdate>20101201</creationdate><title>Emergence and Characterization of Foodborne Methicillin-Resistant Staphylococcus aureus in Korea</title><author>CHAE HONG RHEE ; WOO, Gun-Jo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-e286684f29931ea235fa29d750622f0721a0e655f25a923503320c9084273a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Annealing</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Biological and medical sciences</topic><topic>Colony Count, Microbial</topic><topic>Consumer Product Safety</topic><topic>DNA, Bacterial - analysis</topic><topic>Drug resistance</topic><topic>Electrophoresis</topic><topic>Enterotoxins - genetics</topic><topic>Fish Products - microbiology</topic><topic>Food contamination & poisoning</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Food safety</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genotype</topic><topic>Humans</topic><topic>Korea</topic><topic>Laboratories</topic><topic>Meat Products - microbiology</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - pathogenicity</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Epidemiology</topic><topic>Pathogens</topic><topic>Penicillin</topic><topic>Prevalence</topic><topic>Staphylococcal Food Poisoning - epidemiology</topic><topic>Staphylococcal Food Poisoning - microbiology</topic><topic>Staphylococcus infections</topic><topic>Toxins</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHAE HONG RHEE</creatorcontrib><creatorcontrib>WOO, Gun-Jo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>ABI/INFORM Collection</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Trade & Industry (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Trade & Industry</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Environmental Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of food protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHAE HONG RHEE</au><au>WOO, Gun-Jo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Emergence and Characterization of Foodborne Methicillin-Resistant Staphylococcus aureus in Korea</atitle><jtitle>Journal of food protection</jtitle><addtitle>J Food Prot</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>73</volume><issue>12</issue><spage>2285</spage><epage>2290</epage><pages>2285-2290</pages><issn>0362-028X</issn><eissn>1944-9097</eissn><coden>JFPRDR</coden><abstract>A total of 165 Staphylococcus aureus strains, isolated from different food samples between 2003 and 2006, were tested for antimicrobial susceptibility. The mecA-positive methicillin-resistant S. aureus (MRSA) strains were further characterized by testing for various virulence genes and by molecular typing with multilocus sequence typing and pulsed-field gel electrophoresis. Of the 165 S. aureus isolates, 150 strains (90.9%) were resistant to at least one antibiotic while no strain was resistant to vancomycin. Four strains were resistant to both oxacillin and cefoxitin and were mecA positive. The mecA-positive MRSA strains were isolated from raw meat and fish samples (two beef samples and two fish samples) and were resistant to β-lactam antibiotics. Based on multilocus sequence typing analysis, the isolates were assigned to sequence type 1 (ST1), ST72, and an undetermined ST (ST72 slv). All four MRSA isolates were shown to be enterotoxigenic. The ST1 MRSA isolate harbored the sea-seh gene combination and the ST72 and ST72 slv MRSA strains harbored the seg-sei and the sea-seg-sei gene combinations, respectively. However, none of the MRSA isolates had the genes for Panton-Valentine leukocidin, toxic shock syndrome toxin 1, and exfoliative toxins. The pulsed-field gel electrophoresis patterns of the ST72 isolates in our study were highly similar, even though they were isolated from food samples in different years and from different regions of Korea.</abstract><cop>Des Moines, IA</cop><pub>International Association for Food Protection</pub><pmid>21219750</pmid><doi>10.4315/0362-028X-73.12.2285</doi><tpages>6</tpages></addata></record> |
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| subjects | Annealing Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial agents Biological and medical sciences Colony Count, Microbial Consumer Product Safety DNA, Bacterial - analysis Drug resistance Electrophoresis Enterotoxins - genetics Fish Products - microbiology Food contamination & poisoning Food Contamination - analysis Food industries Food Microbiology Food safety Fundamental and applied biological sciences. Psychology Genes Genotype Humans Korea Laboratories Meat Products - microbiology Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - pathogenicity Microbial Sensitivity Tests Molecular Epidemiology Pathogens Penicillin Prevalence Staphylococcal Food Poisoning - epidemiology Staphylococcal Food Poisoning - microbiology Staphylococcus infections Toxins Virulence |
| title | Emergence and Characterization of Foodborne Methicillin-Resistant Staphylococcus aureus in Korea |
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