In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration
As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways, respectively. Depending on the glucose concentration in the medium, the effect of the addition of H2O2 on the level of ATP and on glyceraldehyde-3-ph...
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| Veröffentlicht in: | Archives of microbiology 2004-03, Vol.181 (3), p.231-236 |
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| creator | Osorio, H Moradas-Ferreira, P Gunther Sillero, M.A Sillero, A |
| description | As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways, respectively. Depending on the glucose concentration in the medium, the effect of the addition of H2O2 on the level of ATP and on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity differed. In the presence of 2% glucose, ATP and GAPDH decreased sharply during the first few minutes of treatment, whereas in the presence of 0.025% glucose, GAPDH activity decreased similarly, but the ATP level remained practically unchanged. The addition of 3 mM glutathione to the culture media prevented the depletion of ATP levels and GAPDH activity in the presence of H2O2. Catalase and superoxide dismutase activities did not vary significantly when yeast cells were grown either in 2% or in 0.025% glucose. |
| doi_str_mv | 10.1007/s00203-004-0648-6 |
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Depending on the glucose concentration in the medium, the effect of the addition of H2O2 on the level of ATP and on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity differed. In the presence of 2% glucose, ATP and GAPDH decreased sharply during the first few minutes of treatment, whereas in the presence of 0.025% glucose, GAPDH activity decreased similarly, but the ATP level remained practically unchanged. The addition of 3 mM glutathione to the culture media prevented the depletion of ATP levels and GAPDH activity in the presence of H2O2. Catalase and superoxide dismutase activities did not vary significantly when yeast cells were grown either in 2% or in 0.025% glucose.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-004-0648-6</identifier><identifier>PMID: 14735298</identifier><identifier>CODEN: AMICCW</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>adenosine triphosphate ; Adenosine Triphosphate - metabolism ; ATP ; Biological and medical sciences ; Catalase - analysis ; Culture media ; Dehydrogenase ; enzyme activity ; Enzymes ; Fundamental and applied biological sciences. Psychology ; fungus physiology ; Glucose ; Glucose - metabolism ; Glutathione - metabolism ; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Hydrogen Peroxide - pharmacology ; Inosine - analysis ; Kinetics ; Microbiology ; Miscellaneous ; Mycology ; Nucleotides - analysis ; Oxidants - pharmacology ; oxidative stress ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - metabolism ; Superoxide Dismutase - analysis ; Yeasts</subject><ispartof>Archives of microbiology, 2004-03, Vol.181 (3), p.231-236</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15493439$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14735298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Osorio, H</creatorcontrib><creatorcontrib>Moradas-Ferreira, P</creatorcontrib><creatorcontrib>Gunther Sillero, M.A</creatorcontrib><creatorcontrib>Sillero, A</creatorcontrib><title>In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways, respectively. Depending on the glucose concentration in the medium, the effect of the addition of H2O2 on the level of ATP and on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity differed. In the presence of 2% glucose, ATP and GAPDH decreased sharply during the first few minutes of treatment, whereas in the presence of 0.025% glucose, GAPDH activity decreased similarly, but the ATP level remained practically unchanged. The addition of 3 mM glutathione to the culture media prevented the depletion of ATP levels and GAPDH activity in the presence of H2O2. Catalase and superoxide dismutase activities did not vary significantly when yeast cells were grown either in 2% or in 0.025% glucose.</description><subject>adenosine triphosphate</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Catalase - analysis</subject><subject>Culture media</subject><subject>Dehydrogenase</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungus physiology</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Glutathione - metabolism</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Inosine - analysis</subject><subject>Kinetics</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Nucleotides - analysis</subject><subject>Oxidants - pharmacology</subject><subject>oxidative stress</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Superoxide Dismutase - analysis</subject><subject>Yeasts</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpFkN1q3DAQhUVpaTZpH6A3rSjkLkpGP7alyxCSJhBIIQn0zmjl0drBK7mSXdjH6BtX22zpzQwzfOcMZwj5xOGcAzQXGUCAZACKQa00q9-QFVdSMGjEj7dkBRIE00bKI3Kc8wsAF1rr9-SIq0ZWwugV-X0X6KN1rrcpbncOM3WY8NeQB4tndO6RovfoZho9vRUPgsZAL5--n9H1MtMQ5_28GYsw2bHDftchk2zqY556OyP9u0pxg8Hm4tfhhKHLe9HeejMuLmakLgaHYU52HmL4QN55O2b8eOgn5Pnm-unqlt0_fLu7urxnvoSYWSlKmG5tDVdaV40Hr6QVCnwjKi8dN6arHHDvDOeyhgocNAZFbdauFlzIE_L11XdK8eeCeW5f4pJCOdlqaKpKF68CfT5Ay3qLXTulYWvTrv33wAKcHgCbnR19ssEN-T9XKSOVNIX78sp5G1u7SYV5fhTAJYCpShaQfwAKEIge</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Osorio, H</creator><creator>Moradas-Ferreira, P</creator><creator>Gunther Sillero, M.A</creator><creator>Sillero, A</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20040301</creationdate><title>In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration</title><author>Osorio, H ; Moradas-Ferreira, P ; Gunther Sillero, M.A ; Sillero, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f288t-288429dba9148857f0f43a240f725f3c199d5c01fc91136050c079e269bc62123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>adenosine triphosphate</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>ATP</topic><topic>Biological and medical sciences</topic><topic>Catalase - analysis</topic><topic>Culture media</topic><topic>Dehydrogenase</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungus physiology</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Glutathione - metabolism</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Inosine - analysis</topic><topic>Kinetics</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Nucleotides - analysis</topic><topic>Oxidants - pharmacology</topic><topic>oxidative stress</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Superoxide Dismutase - analysis</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osorio, H</creatorcontrib><creatorcontrib>Moradas-Ferreira, P</creatorcontrib><creatorcontrib>Gunther Sillero, M.A</creatorcontrib><creatorcontrib>Sillero, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osorio, H</au><au>Moradas-Ferreira, P</au><au>Gunther Sillero, M.A</au><au>Sillero, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration</atitle><jtitle>Archives of microbiology</jtitle><addtitle>Arch Microbiol</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>181</volume><issue>3</issue><spage>231</spage><epage>236</epage><pages>231-236</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><coden>AMICCW</coden><abstract>As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways, respectively. Depending on the glucose concentration in the medium, the effect of the addition of H2O2 on the level of ATP and on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity differed. In the presence of 2% glucose, ATP and GAPDH decreased sharply during the first few minutes of treatment, whereas in the presence of 0.025% glucose, GAPDH activity decreased similarly, but the ATP level remained practically unchanged. The addition of 3 mM glutathione to the culture media prevented the depletion of ATP levels and GAPDH activity in the presence of H2O2. Catalase and superoxide dismutase activities did not vary significantly when yeast cells were grown either in 2% or in 0.025% glucose.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>14735298</pmid><doi>10.1007/s00203-004-0648-6</doi><tpages>6</tpages></addata></record> |
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| subjects | adenosine triphosphate Adenosine Triphosphate - metabolism ATP Biological and medical sciences Catalase - analysis Culture media Dehydrogenase enzyme activity Enzymes Fundamental and applied biological sciences. Psychology fungus physiology Glucose Glucose - metabolism Glutathione - metabolism Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Hydrogen peroxide Hydrogen Peroxide - metabolism Hydrogen Peroxide - pharmacology Inosine - analysis Kinetics Microbiology Miscellaneous Mycology Nucleotides - analysis Oxidants - pharmacology oxidative stress Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - metabolism Superoxide Dismutase - analysis Yeasts |
| title | In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration |
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