Near Infrared Luminescent Oxygen Nanosensors with Nanoparticle Matrix Tailored Sensitivity
The development of sensors for noninvasive determination of oxygen levels in live cells and tissues is critical for the understanding of cellular functions, as well as for monitoring the status of disease, such as cancer, and for predicting the efficacy of therapy. We describe such nontoxic, targete...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2010-10, Vol.82 (20), p.8446-8455 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Koo Lee, Yong-Eun Ulbrich, Elyse E Kim, Gwangseong Hah, Hoejin Strollo, Christen Fan, Wenzhe Gurjar, Rajan Koo, SangMan Kopelman, Raoul |
| description | The development of sensors for noninvasive determination of oxygen levels in live cells and tissues is critical for the understanding of cellular functions, as well as for monitoring the status of disease, such as cancer, and for predicting the efficacy of therapy. We describe such nontoxic, targeted, and ratiometric 30 nm oxygen nanosensors made of polyacrylamide hydrogel, near-infrared (NIR) luminescent dyes, and surface-conjugated tumor-specific peptides. They enabled noninvasive real-time monitoring of oxygen levels in live cancer cells under normal and hypoxic conditions. The required sensitivity, brightness, selectivity, and stability were achieved by tailoring the interaction between the nanomatrix and indicator dyes. The developed nanosensors may become useful for in vivo oxygen measurements. |
| doi_str_mv | 10.1021/ac1015358 |
| format | Article |
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We describe such nontoxic, targeted, and ratiometric 30 nm oxygen nanosensors made of polyacrylamide hydrogel, near-infrared (NIR) luminescent dyes, and surface-conjugated tumor-specific peptides. They enabled noninvasive real-time monitoring of oxygen levels in live cancer cells under normal and hypoxic conditions. The required sensitivity, brightness, selectivity, and stability were achieved by tailoring the interaction between the nanomatrix and indicator dyes. 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Psychology ; General, instrumentation ; Humans ; Hypoxia ; Microscopy, Electron, Scanning ; Molecular and cellular biology ; Molecular Structure ; Nanoparticles ; Nanoparticles - analysis ; Nanoparticles - ultrastructure ; Oxygen ; Oxygen - analysis ; Sensors ; Tissues</subject><ispartof>Analytical chemistry (Washington), 2010-10, Vol.82 (20), p.8446-8455</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Oct 15, 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a406t-bc1213704d44aff0b5fa1f1c8ea43d2aafe0dac8893be0b8061f7400919f088c3</citedby><cites>FETCH-LOGICAL-a406t-bc1213704d44aff0b5fa1f1c8ea43d2aafe0dac8893be0b8061f7400919f088c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac1015358$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac1015358$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23325734$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20849084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koo Lee, Yong-Eun</creatorcontrib><creatorcontrib>Ulbrich, Elyse E</creatorcontrib><creatorcontrib>Kim, Gwangseong</creatorcontrib><creatorcontrib>Hah, Hoejin</creatorcontrib><creatorcontrib>Strollo, Christen</creatorcontrib><creatorcontrib>Fan, Wenzhe</creatorcontrib><creatorcontrib>Gurjar, Rajan</creatorcontrib><creatorcontrib>Koo, SangMan</creatorcontrib><creatorcontrib>Kopelman, Raoul</creatorcontrib><title>Near Infrared Luminescent Oxygen Nanosensors with Nanoparticle Matrix Tailored Sensitivity</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The development of sensors for noninvasive determination of oxygen levels in live cells and tissues is critical for the understanding of cellular functions, as well as for monitoring the status of disease, such as cancer, and for predicting the efficacy of therapy. We describe such nontoxic, targeted, and ratiometric 30 nm oxygen nanosensors made of polyacrylamide hydrogel, near-infrared (NIR) luminescent dyes, and surface-conjugated tumor-specific peptides. They enabled noninvasive real-time monitoring of oxygen levels in live cancer cells under normal and hypoxic conditions. The required sensitivity, brightness, selectivity, and stability were achieved by tailoring the interaction between the nanomatrix and indicator dyes. The developed nanosensors may become useful for in vivo oxygen measurements.</description><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Cell Line, Tumor</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cells</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fluorescent Dyes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Microscopy, Electron, Scanning</subject><subject>Molecular and cellular biology</subject><subject>Molecular Structure</subject><subject>Nanoparticles</subject><subject>Nanoparticles - analysis</subject><subject>Nanoparticles - ultrastructure</subject><subject>Oxygen</subject><subject>Oxygen - analysis</subject><subject>Sensors</subject><subject>Tissues</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0E1LAzEQBuAgiq3Vg39AFsGDh9VJsh_ZoxQ_CtUerBcvy2w20ZR2tya72v57U1tbwUMYCA_vDC8hpxSuKDB6jZICjXks9kiXxgzCRAi2T7oAwEOWAnTIkXMTAOpdckg6DESU-dclr08KbTCotEWrymDYzkylnFRVE4wWyzdVBU9Y1U5VrrYu-DLN-8_HHG1j5FQFj9hYswjGaKb1KuDZS9OYT9Msj8mBxqlTJ5vZIy93t-P-Qzgc3Q_6N8MQI0iasJCUUZ5CVEYRag1FrJFqKoXCiJcMUSsoUQqR8UJBISChOo0AMpppEELyHjlf585t_dEq1-STurWVX5kL304igDKPLtdI2to5q3Q-t2aGdplTyFcl5tsSvT3bBLbFTJVb-duaBxcbgE7i1HdXSeN2jnMWp_yPQ-l2R_1f-A2zTYVD</recordid><startdate>20101015</startdate><enddate>20101015</enddate><creator>Koo Lee, Yong-Eun</creator><creator>Ulbrich, Elyse E</creator><creator>Kim, Gwangseong</creator><creator>Hah, Hoejin</creator><creator>Strollo, Christen</creator><creator>Fan, Wenzhe</creator><creator>Gurjar, Rajan</creator><creator>Koo, SangMan</creator><creator>Kopelman, Raoul</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>20101015</creationdate><title>Near Infrared Luminescent Oxygen Nanosensors with Nanoparticle Matrix Tailored Sensitivity</title><author>Koo Lee, Yong-Eun ; Ulbrich, Elyse E ; Kim, Gwangseong ; Hah, Hoejin ; Strollo, Christen ; Fan, Wenzhe ; Gurjar, Rajan ; Koo, SangMan ; Kopelman, Raoul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a406t-bc1213704d44aff0b5fa1f1c8ea43d2aafe0dac8893be0b8061f7400919f088c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Cell Line, Tumor</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cells</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fluorescent Dyes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Humans</topic><topic>Hypoxia</topic><topic>Microscopy, Electron, Scanning</topic><topic>Molecular and cellular biology</topic><topic>Molecular Structure</topic><topic>Nanoparticles</topic><topic>Nanoparticles - analysis</topic><topic>Nanoparticles - ultrastructure</topic><topic>Oxygen</topic><topic>Oxygen - analysis</topic><topic>Sensors</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koo Lee, Yong-Eun</creatorcontrib><creatorcontrib>Ulbrich, Elyse E</creatorcontrib><creatorcontrib>Kim, Gwangseong</creatorcontrib><creatorcontrib>Hah, Hoejin</creatorcontrib><creatorcontrib>Strollo, Christen</creatorcontrib><creatorcontrib>Fan, Wenzhe</creatorcontrib><creatorcontrib>Gurjar, Rajan</creatorcontrib><creatorcontrib>Koo, SangMan</creatorcontrib><creatorcontrib>Kopelman, Raoul</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koo Lee, Yong-Eun</au><au>Ulbrich, Elyse E</au><au>Kim, Gwangseong</au><au>Hah, Hoejin</au><au>Strollo, Christen</au><au>Fan, Wenzhe</au><au>Gurjar, Rajan</au><au>Koo, SangMan</au><au>Kopelman, Raoul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Near Infrared Luminescent Oxygen Nanosensors with Nanoparticle Matrix Tailored Sensitivity</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| subjects | Analytical chemistry Biological and medical sciences Calibration Cell Line, Tumor Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cells Chemistry Exact sciences and technology Fluorescent Dyes - analysis Fundamental and applied biological sciences. Psychology General, instrumentation Humans Hypoxia Microscopy, Electron, Scanning Molecular and cellular biology Molecular Structure Nanoparticles Nanoparticles - analysis Nanoparticles - ultrastructure Oxygen Oxygen - analysis Sensors Tissues |
| title | Near Infrared Luminescent Oxygen Nanosensors with Nanoparticle Matrix Tailored Sensitivity |
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