Evaluation of Virulence Factor Profiling in the Characterization of Veterinary Escherichia coli Isolates
Escherichia coli has been used as an indicator organism for fecal contamination of water and other environments and is often a commensal organism in healthy animals, yet a number of strains can cause disease in young or immunocompromised animals. In this study, 281 E. coli isolates from bovine, porc...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2010-11, Vol.76 (22), p.7509-7513 |
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| creator | David, Donna E Lynne, Aaron M Han, Jing Foley, Steven L |
| description | Escherichia coli has been used as an indicator organism for fecal contamination of water and other environments and is often a commensal organism in healthy animals, yet a number of strains can cause disease in young or immunocompromised animals. In this study, 281 E. coli isolates from bovine, porcine, chicken, canine, equine, feline, and other veterinary sources were analyzed by BOXA1R PCR and by virulence factor profiling of 35 factors to determine whether they had utility in identifying the animal source of the isolates. The results of BOXA1R PCR analysis demonstrated a high degree of diversity; less than half of the isolates fell into one of 27 clusters with at least three isolates (based on 90% similarity). Nearly 60% of these clusters contained isolates from more than one animal source. Conversely, the results of virulence factor profiling demonstrated clustering by animal source. Three clusters, named Bovine, Chicken, and Porcine, based on discriminant components analysis, were represented by 90% or more of the respective isolates. A fourth group, termed Companion, was the most diverse, containing at least 84% of isolates from canine, feline, equine, and other animal sources. Based on these results, it appears that virulence factor profiling may have utility, helping identify the likely animal host species sources of certain E. coli isolates. |
| doi_str_mv | 10.1128/AEM.00726-10 |
| format | Article |
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In this study, 281 E. coli isolates from bovine, porcine, chicken, canine, equine, feline, and other veterinary sources were analyzed by BOXA1R PCR and by virulence factor profiling of 35 factors to determine whether they had utility in identifying the animal source of the isolates. The results of BOXA1R PCR analysis demonstrated a high degree of diversity; less than half of the isolates fell into one of 27 clusters with at least three isolates (based on 90% similarity). Nearly 60% of these clusters contained isolates from more than one animal source. Conversely, the results of virulence factor profiling demonstrated clustering by animal source. Three clusters, named Bovine, Chicken, and Porcine, based on discriminant components analysis, were represented by 90% or more of the respective isolates. A fourth group, termed Companion, was the most diverse, containing at least 84% of isolates from canine, feline, equine, and other animal sources. 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Based on these results, it appears that virulence factor profiling may have utility, helping identify the likely animal host species sources of certain E. coli isolates.</description><subject>Animals</subject><subject>Animals, Domestic</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriological Techniques - methods</subject><subject>Cluster Analysis</subject><subject>Contamination</subject><subject>E coli</subject><subject>Environmental conditions</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Feces</subject><subject>Genetic Variation</subject><subject>Indicator organisms</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Public Health Microbiology</subject><subject>Virulence Factors - genetics</subject><subject>Water quality</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9vFCEUx4nR2G315lmJFy-d-oAZflxMms1Wm7SpidYrYVjYoZkZKszU2L9etltb9WI4POB93pf3-CL0isARIVS-P16dHwEIyisCT9CCgJJVwxh_ihYASlWU1rCH9nO-AoAauHyO9ihIqYSCBepWN6afzRTiiKPH30Kaezdah0-MnWLCn1P0oQ_jBocRT53Dy86kknIp3D5Wue15NOknXmXblb3tgsE29gGf5tibyeUX6Jk3fXYv7-MBujxZfV1-qs4uPp4uj88qWys2VcJzShtac8lE04q2VlSBB0I8JWXKFgglVqxbB5xYo3wrGJFrVZYvkRt2gD7sdK_ndnBr68YpmV5fpzCU_nQ0Qf-dGUOnN_FGUyU4BVoE3t0LpPh9dnnSQ8jW9b0ZXZyzlkxxCUzAf0nBGeFN3chCvv2HvIpzGss_aAkUBFCyhQ53kE0x5-T8Q9ME9NZqXazWd1aXm4K__nPQB_i3t4-PdmHT_QjJaZMHbdxQGtOUatGAKtCbHeRN1GaTQtaXXygQBkSBYJywX0IFuMQ</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>David, Donna E</creator><creator>Lynne, Aaron M</creator><creator>Han, Jing</creator><creator>Foley, Steven L</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope><scope>5PM</scope></search><sort><creationdate>20101101</creationdate><title>Evaluation of Virulence Factor Profiling in the Characterization of Veterinary Escherichia coli Isolates</title><author>David, Donna E ; Lynne, Aaron M ; Han, Jing ; Foley, Steven L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-7f62252468375b7b49290f011f21007b0121c7dbe061ca9fb7318d9d9df18d6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Animals, Domestic</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriological Techniques - methods</topic><topic>Cluster Analysis</topic><topic>Contamination</topic><topic>E coli</topic><topic>Environmental conditions</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Feces</topic><topic>Genetic Variation</topic><topic>Indicator organisms</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Public Health Microbiology</topic><topic>Virulence Factors - genetics</topic><topic>Water quality</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>David, Donna E</creatorcontrib><creatorcontrib>Lynne, Aaron M</creatorcontrib><creatorcontrib>Han, Jing</creatorcontrib><creatorcontrib>Foley, Steven L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>David, Donna E</au><au>Lynne, Aaron M</au><au>Han, Jing</au><au>Foley, Steven L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Virulence Factor Profiling in the Characterization of Veterinary Escherichia coli Isolates</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>76</volume><issue>22</issue><spage>7509</spage><epage>7513</epage><pages>7509-7513</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Escherichia coli has been used as an indicator organism for fecal contamination of water and other environments and is often a commensal organism in healthy animals, yet a number of strains can cause disease in young or immunocompromised animals. 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| ispartof | Applied and Environmental Microbiology, 2010-11, Vol.76 (22), p.7509-7513 |
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| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Animals, Domestic Bacterial Typing Techniques Bacteriological Techniques - methods Cluster Analysis Contamination E coli Environmental conditions Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - pathogenicity Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli Proteins - genetics Feces Genetic Variation Indicator organisms Polymerase Chain Reaction - methods Public Health Microbiology Virulence Factors - genetics Water quality |
| title | Evaluation of Virulence Factor Profiling in the Characterization of Veterinary Escherichia coli Isolates |
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